In-Fusion Cloning tutorials
Our In‑Fusion Cloning Primer Design Tool lets you quickly and effortlessly plan out any seamless cloning project using In‑Fusion Cloning technology. The online tool is as flexible as In‑Fusion Cloning itself, accommodating:
- single- or multiple-insert cloning without scar sequences,
- vector linearization by inverse PCR or restriction digest, and
- site-directed mutagenesis (insertions, deletions, substitutions).
Each step is marked for easy addition of your sequences and project specifications. Map and sequence panes let you visualize your vector as you go, and results include a final map and output protocol with oligo and PCR information. The following tutorials guide you through each step of various In‑Fusion Cloning applications. General information and help are also available below the tutorials.
Design primers to seamlessly clone single or multiple inserts directly into your destination vector at any locus.
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