In-Fusion Cloning webinars
Cloning is an integral starting point for the vast majority of research done in biological sciences. What if you could spend less time on this step, and more time actually running experiments? Our In-Fusion Snap Assembly Master Mixes and bundles are easy to use and highly accurate, making it possible for you to recover your final vector without any subcloning. You've put a lot of thought into your plasmid designs, and In‑Fusion technology can make them a reality with one quick reaction.
Our cloning specialists have created the following presentations to illustrate the benefits and best practices of choosing In-Fusion Cloning for your next cloning or mutagenesis project.
Automating In-Fusion on the Eppendorf epMotion®
To achieve automated, high-throughput cloning, Takara Bio has partnered with Eppendorf to seamlessly integrate In-Fusion Snap Assembly cloning with the epMotion® automated liquid handling platform. A perfect team, these two technologies provide researchers with a reliable, accurate, scalable, and consistent approach to high-throughput cloning. Our simple protocol allows you to:
- Perform high-throughput cloning—choose from a 96- or 384-well plate
- Scale up while saving time—process 40 samples in just 15 min with the epMotion, and incubate in only 15 min with our In-Fusion Cloning kit
- Enhance reproducibility—achieve consistent results; in our trials, 100% of samples yielded successfully cloned colonies
- Reduce reaction volumes and pipetting errors—save money on reagents with automated pipetting and lower laboratory staff’s hands-on time
Cloning dsDNA fragments as an alternative to PCR products
This video will show you how to design In-Fusion-ready double-stranded DNA fragments to seamlessly clone into your vector. We use the pEmpty Vector and the vectors included within the Lenti-X SARS-CoV-2 Spike Vector Set, which are included in the Lenti-X SARS-CoV-2 Packaging Single Shots (Universal) kit, as an example.
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