In-Fusion Cloning guide

In-Fusion PCR Cloning systems enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector with high accuracy and high fidelity. No additional treatment of the PCR fragment—such as restriction digestion, ligation, phosphorylation, or blunt-end polishing—is needed. The efficiency of In-Fusion Cloning kits is over 95%, and just one quick reaction allows you to recover your final construct. This versatile system easily outpaces any traditional cloning method, including ligation and TA cloning.

Which kit best fits my needs?

Features	In-Fusion Snap Assembly	In-Fusion Snap Assembly EcoDry
Format	Liquid	Lyophilized
Ability to customize reaction volume and/or plasticware
Easily scalable for high-throughput applications
Minimize handling with prealiquoted, lyophilized components
Store reagents at room temperature
Compatible with Cloning Enhancer for fast, enzymatic removal of background DNA and PCR residue when PCR produces a single, clean band
Compatible with PCR purification and gel extraction kits (such as NucleoSpin Gel and PCR Clean-up kits) when PCR produces >1 product

All In-Fusion Snap Assembly and Snap Assembly EcoDry products contain reagents for control experiments (linearized vector and a 2-kb control insert).

Save time	Be efficient	Don’t compromise on flexibility
One 15-minute reaction for single or multiple fragments	Single-insert cloning yields 95–100%	Clone any insert into any location within any vector
No additional treatment of PCR fragments required	Multiple-insert cloning yields 90–100%	Seamless cloning—no extra base pairs left over
No subcloning—get your final construct on the second day	Large inserts or vectors are not a problem—clone directly into your expression plasmid	Primer design lets you easily preserve or eliminate restriction sites at cloning junctions

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