Titanium Taq PCR kit
The Titanium Taq PCR Kit contains our high-yield Titanium Taq polymerase blend plus 10X buffer, dNTP mixture, and control DNA template and primers.
The Titanium Taq PCR Kit contains our high-yield Titanium Taq polymerase blend plus 10X buffer, dNTP mixture, and control DNA template and primers.
Titanium Taq is a mixture consisting of a 5' exonuclease-deficient Taq polymerase and TaqStart Antibody for hot-start PCR. Titanium Taq is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It is more sensitive and robust than other polymerases due to its higher solubility and the integrated hot-start antibody, which increases PCR specificity and yield (Kellogg et al. 1994).
Overview
- High yield—requires fewer PCR cycles.
- Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization.
- Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets.
- Integrated hot-start antibody for enhanced specificity—minimizes the formation of primer dimers and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature.
More Information
Applications
- Hot-start PCR
- Multiplex PCR
- Rare-template amplification
- Whole-genome PCR
- Standard PCR (e.g., genotyping, primer extension, RT-PCR, and screening clones)
References
Kellogg, D. E. et al. Taqstart Antibody: “Hot Start” PCR Facilitated by a Neutralizing Monoclonal Antibody Directed Against Taq DNA Polymerase. BioTechniques 16, 1137 (1994).
Product citations
Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).
Kim, Y. et al. Crystal structure of Thermus aquaticus DNA polymerase. Nature 376, 612–616 (1995).
Additional product information
Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Amplifying our understanding of breast cancer metastases
Learn more about how our Titanium Taq provided robust and sensitive amplification in breast cancer research.
Tech notes
Titanium Taq outperforms other polymerases in high-throughput genotyping
Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.
Learn more about whole-genome SNP genotyping with Titanium Taq
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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