Titanium Taq PCR kit

The Titanium Taq PCR Kit contains our high-yield Titanium Taq polymerase blend plus 10X buffer, dNTP mixture, and control DNA template and primers.

Titanium Taq is a mixture consisting of a 5' exonuclease-deficient Taq polymerase and TaqStart Antibody for hot-start PCR. Titanium Taq is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It is more sensitive and robust than other polymerases due to its higher solubility and the integrated hot-start antibody, which increases PCR specificity and yield (Kellogg et al. 1994).

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Cat. #	Product	Size	Price
639210	Titanium® Taq PCR Kit	100 Rxns	$282.00
This complete kit contains all the reagents needed for PCR, including control template and primer mix. Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStat® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Titanium Taq works with a wide range of samples, and is active over a wide range of Mg²⁺ concentrations. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Titanium Taq efficiently amplifies specific genes from genomic DNA *Titanium Taq* efficiently amplifies specific genes from genomic DNA.** Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder. Back TITANIUM Taq is active over a wide range of Mg2+ concentrations *TITANIUM Taq* is active over a wide range of Mg²⁺ concentrations.** TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl₂ concentration was varied as indicated. The enzyme performed consistently through the range of Mg²⁺ used. Lane M: DNA size marker. Back Titanium Taq PCR KitCatalog No.639210 Titanium Taq PCR Kit Catalog No.639210.
639211	Titanium® Taq PCR Kit	30 Rxns	$141.00
This complete kit contains all the reagents needed for PCR, including control template and primer mix. Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStar® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Titanium Taq works with a wide range of samples, and is active over a wide range of Mg²⁺ concentrations. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Titanium Taq efficiently amplifies specific genes from genomic DNA *Titanium Taq* efficiently amplifies specific genes from genomic DNA.** Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder. Back TITANIUM Taq is active over a wide range of Mg2+ concentrations *TITANIUM Taq* is active over a wide range of Mg²⁺ concentrations.** TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl₂ concentration was varied as indicated. The enzyme performed consistently through the range of Mg²⁺ used. Lane M: DNA size marker.

Overview

High yield—requires fewer PCR cycles.
Active over wide ranges of Mg²⁺ concentrations—minimizes time spent on optimization.
Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets.
Integrated hot-start antibody for enhanced specificity—minimizes the formation of primer dimers and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature.

More Information

Applications

Hot-start PCR
Multiplex PCR
Rare-template amplification
Whole-genome PCR
Standard PCR (e.g., genotyping, primer extension, RT-PCR, and screening clones)

References

Kellogg, D. E. et al. Taqstart Antibody: “Hot Start” PCR Facilitated by a Neutralizing Monoclonal Antibody Directed Against Taq DNA Polymerase. BioTechniques 16, 1137 (1994).

Product citations

Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).

Kim, Y. et al. Crystal structure of Thermus aquaticus DNA polymerase. Nature 376, 612–616 (1995).

Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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