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Home › Products › PCR › High-yield PCR › Titanium Taq products › Titanium Taq PCR kit

High-yield PCR

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Titanium Taq PCR kit

high-yield PCR

The Titanium Taq PCR Kit contains our high-yield Titanium Taq polymerase blend plus 10X buffer, dNTP mixture, and control DNA template and primers.

The Titanium Taq PCR Kit contains our high-yield Titanium Taq polymerase blend plus 10X buffer, dNTP mixture, and control DNA template and primers.

Titanium Taq is a mixture consisting of a 5' exonuclease-deficient Taq polymerase and TaqStart Antibody for hot-start PCR. Titanium Taq is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It is more sensitive and robust than other polymerases due to its higher solubility and the integrated hot-start antibody, which increases PCR specificity and yield (Kellogg et al. 1994).

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Cat. # Product Size Price License Quantity Details
639210 Titanium® Taq PCR Kit 100 Rxns USD $300.00

This complete kit contains all the reagents needed for PCR, including control template and primer mix. Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStat® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Titanium Taq works with a wide range of samples, and is active over a wide range of Mg2+ concentrations.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.

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TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.

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639210: Titanium Taq PCR Kit

639210: Titanium Taq PCR Kit
639211 Titanium® Taq PCR Kit 30 Rxns USD $151.00

This complete kit contains all the reagents needed for PCR, including control template and primer mix. Titanium Taq DNA Polymerase is specially engineered for higher robustness and sensitivity than wild-type Taq, and contains TaqStar® Antibody for hot-start PCR. The polymerase mix is suitable for use in all PCR applications. Titanium Taq works with a wide range of samples, and is active over a wide range of Mg2+ concentrations.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.

Back

TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.

Back

639211: Titanium Taq PCR Kit

639211: Titanium Taq PCR Kit

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • High yield—requires fewer PCR cycles.
  • Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization.
  • Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets.
  • Integrated hot-start antibody for enhanced specificity—minimizes the formation of primer dimers and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature.

More Information

Applications

  • Hot-start PCR
  • Multiplex PCR
  • Rare-template amplification
  • Whole-genome PCR
  • Standard PCR (e.g., genotyping, primer extension, RT-PCR, and screening clones)

References

Kellogg, D. E. et al. Taqstart Antibody: “Hot Start” PCR Facilitated by a Neutralizing Monoclonal Antibody Directed Against Taq DNA Polymerase. BioTechniques 16, 1137 (1994).

Product citations

Barnes, W. M. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112, 29–35 (1992).

Kim, Y. et al. Crystal structure of Thermus aquaticus DNA polymerase. Nature 376, 612–616 (1995).

Additional product information

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


Amplifying our understanding of breast cancer metastases

Learn more about how our Titanium Taq provided robust and sensitive amplification in breast cancer research.

Read more


Tech notes

Titanium Taq outperforms other polymerases in high-throughput genotyping

Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.

Learn more about whole-genome SNP genotyping with Titanium Taq


pcr success PCR learning center
Selection guides PCR selection guide
Citations Citations: Titanium Taq

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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