TaqStart Antibody: turn any Taq into a hot-start Taq

TaqStart Antibody provides antibody-mediated hot start that enhances the specificity and sensitivity of PCR. This antibody inhibits polymerase activity before the onset of thermal cycling, preventing nonspecific amplification and primer-dimer formation. When the reaction temperature is raised, the antibody is quickly inactivated and PCR proceeds. Dilution buffer is provided.

TaqStart Antibody is significantly more convenient to use than other hot-start methods and offers several advantages:

Avoids sample damage due to depurination that can occur with the high-temperature incubations that are necessary to activate some hot-start enzymes (Fromenty et al. 2000; Barnes 1994; Fromenty et al. 1996; Friedberg, Walker, and Siede 1995; Lindahl and Andersson 1972; Suzuki, Ohsumi, and Makino 1994).
Reduces the risk of cross-contamination because it is not necessary to reopen the reaction tubes after heating.
Can be used when other hot-start methods are difficult to perform, such as for high-throughput PCR, in situ PCR, microtiter-plate formats, capillary PCR, and oil or wax-free environments.
Provides more definitive PCR results in cases when amplification of nonspecific products is a problem, such as reactions involving low copy number targets, complex DNA background, or degenerate primers.

TaqStart Antibody is effective with any Taq-derived DNA polymerase (native, recombinant, and N-terminal deletion mutants). Our Titanium Taq DNA Polymerase and all of our Advantage HD and Advantage 2 products include TaqStart Antibody.

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Cat. #	Product	Size	Price
639251	TaqStart® Antibody	500 Rxns	$403.00
A monoclonal antibody (isotype IgG2b) that specifically binds to and inactivates Taq DNA polymerase below 70°C for use in hot-start PCR. PCR with TaqStart Antibody has been proven to prevent generation of nonspecific amplification products and primer-dimer artifacts. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Increased yield and specificity of RT-PCR with TaqStart Antibody Increased yield and specificity of RT-PCR with TaqStart Antibody. cDNA templates were reverse transcribed using oligo(dT)₁₈ primers and 2 μg of total RNA isolated from human PHA-stimulated peripheral blood leukocytes or mouse RAW 264.7 macrophage cells. Taq DNA Polymerase was added either with (+) or without (–) TaqStart Antibody for 35 cycles. PCR products were run on a 1.8% agarose/EtBr gel. Lane 1: human c-jun; 410 bp. Lane 2: mouse IL-2 receptor; 700 bp. Lane 3: mouse inducible nitric oxide synthase; 497 bp. Lane M: FX174/Hae III DNA size marker. Back TaqStart Antibody Catalog No.639251 TaqStart Antibody Catalog No.639251. Back TaqStart Antibody inhibits polymerase activity before thermal cycling begins TaqStart Antibody inhibits polymerase activity before thermal cycling begins.
639250	TaqStart® Antibody	200 Rxns	$216.00
A monoclonal antibody (isotype IgG2b) that specifically binds to and inactivates Taq DNA polymerase below 70°C for use in hot-start PCR. PCR with TaqStart Antibody has been proven to prevent generation of nonspecific amplification products and primer-dimer artifacts. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Increased yield and specificity of RT-PCR with TaqStart Antibody Increased yield and specificity of RT-PCR with TaqStart Antibody. cDNA templates were reverse transcribed using oligo(dT)₁₈ primers and 2 μg of total RNA isolated from human PHA-stimulated peripheral blood leukocytes or mouse RAW 264.7 macrophage cells. Taq DNA Polymerase was added either with (+) or without (–) TaqStart Antibody for 35 cycles. PCR products were run on a 1.8% agarose/EtBr gel. Lane 1: human c-jun; 410 bp. Lane 2: mouse IL-2 receptor; 700 bp. Lane 3: mouse inducible nitric oxide synthase; 497 bp. Lane M: FX174/Hae III DNA size marker. Back TaqStart Antibody inhibits polymerase activity before thermal cycling begins TaqStart Antibody inhibits polymerase activity before thermal cycling begins.

Overview

Allows convenient room temperature reaction setup
Provides increased specificity, which leads to reduced background, allowing you to obtain your desired amplification product the first time
Effective and inexpensive method for hot-start PCR
Antibody can be used with any full-length Taq polymerase
Available in bulk quantities

More Information

Applications

Hot-start PCR

References

Barnes, W. M. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proc. Natl. Acad. Sci. U. S. A. 91, 2216–20 (1994).

Friedberg, E. C., Walker, G. C. & Siede, W. DNA repair and mutagenesis (ASM Press, 1995).

Fromenty, B. et al. Most cases of medium-chain acyl-CoA dehydrogenase deficiency escape detection in France. Hum. Genet. 97, 367–8 (1996).

Fromenty, B., Demeilliers, C., Mansouri, A. & Pessayre, D. Escherichia coli exonuclease III enhances long PCR amplification of damaged DNA templates. Nucleic Acids Res. 28, E50 (2000).

Lindahl, T. & Andersson, A. Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid. Biochemistry 11, 3618–23 (1972).

Suzuki, T., Ohsumi, S. & Makino, K. Mechanistic studies on depurination and apurinic site chain breakage in oligodeoxyribonucleotides. Nucleic Acids Res. 22, 4997–5003 (1994).

Product citations

Gerard, G. F., D'Alessio, J. M. in Methods Mol. Biol. (Burrell, M. M.) 73–94 (Humana Press, 1993).

Kellogg, D. E. et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16, 1134–7 (1994).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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