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High-yield PCR

Advantage 2 Polymerase Mix

Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.

Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.

Advantage 2 Polymerase Mix is perfect for PCR applications requiring accuracy, such as RACE PCR, cDNA amplification, library construction, and PCR subtraction. Advantage 2 Polymerase Mix is highly recommended for applications downstream of our SMART(er) method for cDNA synthesis.

Advantage 2 Polymerase Mix is also available in a complete PCR kit that includes dNTPs and PCR controls, or in a ready-to-use, lyophilized format (High Fidelity PCR EcoDry Premix).

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Cat. #	Product	Size	Price
639201	Advantage® 2 Polymerase Mix	100 Rxns	USD $323.00
Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639201: Advantage 2 Polymerase Mix
639202	Advantage® 2 Polymerase Mix	5 x 100 Rxns	USD $1290.00
Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 500 PCR reactions of 50 μl each. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Back Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix *Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq* polymerase mix.** 5 μl of PCR products were run on a 1.1% agarose/EtBr gel. Lane 1: The 0.4-kb TNFR II fragment is readily obtained with Advantage 2. Lane 2: No product is seen with Taq polymerase. Lane M: DNA size marker Back Amplification of various large templates using Advantage 2 Polymerase Mix Amplification of various large templates using Advantage 2 Polymerase Mix. 1–3 μl of each PCR product was run on a 1.1% agarose/EtBr gel. Lane 1: 2.5-kb E. coli DNA polymerase gene amplified from genomic DNA. Lane 2: 3.5-kb bovine pancreatic trypsin inhibitor gene amplified from calf thymus genomic DNA. Lane 3: 5.9-kb human IL-1β gene amplified from human genomic DNA. Lane 4: 8.5-kb human titin cDNA amplified from a SMART Human Skeletal Muscle cDNA library. Lane 5: 18.5-kb λ insert amplified from a λ clone. Lane M: λ/Hind III DNA size marker. Back 639202: Advantage 2 Polymerase Mix
639282	High Fidelity PCR EcoDry™ Premix	24 Rxns	USD $77.00
A lyophilized PCR master mix based on Takara's hot-start Advantage 2 Polymerase Mix. The premix contains all neceeary reagents for a high-yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps. Simply reconstitute the mix by adding PCR-grade water along with primers and template. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps. Back 639282: High Fidelity PCR EcoDry Premix
639280	High Fidelity PCR EcoDry™ Premix	48 Rxns	USD $127.00
A lyophilized PCR master mix based on Takara's hot-start Advantage 2 Polymerase Mix. The premix contains all neceeary reagents for a high yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps. Simply reconstitute the mix by adding PCR-grade water along with primers and template. Cat. # 639280 contains 2 of Cat. # 639282. Please refer to Cat. # 639282 for complete product documentation and resources. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps. Back 639280: High Fidelity PCR EcoDry Premix

Overview

Unique blend of enzymes provides nearly error-free amplification—almost 3-fold higher fidelity than wild-type Taq
Exceptionally high yields and sensitivity with almost any target DNA up to 5 kb
Pre-optimized buffer eliminates the need to optimize reaction conditions
Integrated hot-start Ab allows room temperature reaction setup and increased specificity
Available in a lyophilized format as High Fidelity PCR EcoDry Premix, which is convenient for room-temperature shipment and storage

More Information

Applications

Long and accurate PCR
Preparative PCR
RACE PCR
cDNA synthesis/amplification
cDNA subtraction
Hot-start PCR

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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Advantage 2 Polymerase Mix

Notice to purchaser

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639201: Advantage 2 Polymerase Mix

Notice to purchaser

Amplification of a fragment of the rare tumor necrosis factor receptor II (TNFR II) cDNA with Advantage 2 Polymerase Mix and a competitor's Taq polymerase mix

Amplification of various large templates using Advantage 2 Polymerase Mix

639202: Advantage 2 Polymerase Mix

Notice to purchaser

EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps

639282: High Fidelity PCR EcoDry Premix

Notice to purchaser

EcoDry Premixes are packaged in individual 8-well tube-strips with optically clear sealing caps

639280: High Fidelity PCR EcoDry Premix

Overview

More Information

Applications

Bulk, custom, and OEM information

Additional product information

Find answers to your PCR questions