Advantage 2 Polymerase Mix
Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.
Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.
Advantage 2 Polymerase Mix is perfect for PCR applications requiring accuracy, such as RACE PCR, cDNA amplification, library construction, and PCR subtraction. Advantage 2 Polymerase Mix is highly recommended for applications downstream of our SMART(er) method for cDNA synthesis.
Advantage 2 Polymerase Mix is also available in a complete PCR kit that includes dNTPs and PCR controls, or in a ready-to-use, lyophilized format (High Fidelity PCR EcoDry Premix).
Overview
- Unique blend of enzymes provides nearly error-free amplification—almost 3-fold higher fidelity than wild-type Taq
- Exceptionally high yields and sensitivity with almost any target DNA up to 5 kb
- Pre-optimized buffer eliminates the need to optimize reaction conditions
- Integrated hot-start Ab allows room temperature reaction setup and increased specificity
- Available in a lyophilized format as High Fidelity PCR EcoDry Premix, which is convenient for room-temperature shipment and storage
More Information
Applications
- Long and accurate PCR
- Preparative PCR
- RACE PCR
- cDNA synthesis/amplification
- cDNA subtraction
- Hot-start PCR
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
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There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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