- Ideal for high-fidelity, high-yield endpoint PCR
Nicking activity, endonuclease, and exonuclease activity are not detected after incubation of 0.6 µg of supercoiled pBR322 DNA, 0.6 mg of lambda DNA, or 0.6 µg of lambda-HindIII digest with 10 units of this enzyme for 1 hour at 74°C.
Most PCR products (approximately 80%) amplified with Takara Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.
PCR performance test
PCR performance is confirmed by using lambda DNA as the template (amplified fragment: 20 kb).
PCR performance is also confirmed via amplification of the beta-globin gene from human DNA template (amplified fragment: 17.5 kb).
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at email@example.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2019 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.