Ex Taq DNA polymerase—a robust PCR enzyme with proofreading activity
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.
TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'-to-5' exonuclease, for high-sensitivity, high-efficiency PCR reactions. It can also be used for long-range PCR (up to 20 kb from genomic DNA templates and up to 30 kb from lambda DNA templates). Ex Taq polymerase has a higher fidelity than standard Taq with a mutation rate approximately 4.5 times lower, as determined by the Kunkel method. Ex Taq polymerase is supplied with optimized 10X buffer (with or without Mg2+) and dNTPs.
In routine PCR applications, using Ex Taq polymerase and the Ex Taq buffer system results in higher yields of PCR products as compared to standard Taq DNA polymerase.
Several other formats of Ex Taq are available:
- An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
- A 2X PCR master mix contains Ex Taq enzyme, reaction buffer (with Mg2+), and dNTPs—for a simple PCR setup and minimal pipetting steps. A loading-dye added versions, PerfectShot Ex Taq DNA Polymerase, is also available for convenient loading of PCR products in agarose gels directly.
Overview
- DNA-proofreading activity allows for high-fidelity and high-sensitivity reactions
- Obtain a wide range of PCR products (<100 bp to 20 kb for genomic DNA targets)
- Optimized 10X Buffer and dNTPs are included—less optimization and more reproducible results than Taq
More Information
Applications
- Ideal for high-fidelity, high-yield endpoint PCR
PCR products
Most PCR products (approximately 80%) amplified with Takara Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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1.17 | Heading into bat caves on a rescue mission
Bats may have a "spooky" reputation, but one bat disease is causing widespread fear among wildlife biologists: White Nose Syndrome caused by the fungus Geomyces destructans. With a mortality rate near 100%, the disease is devastating bat populations. Daniel Lindner and colleagues used Takara Ex Taq polymerase to screen for G. destructans in soil samples from bat hibernacula, gathering data and developing testing methods to help bats everywhere. That's Good Science!
See what our customers are saying about Takara Ex Taq DNA Polymerase!
"Takara Ex Taq amplify better than pfu from Stratagene, and also has high fidelity. I prefer Ex Taq than pfu."
—Qin Yan, DUKE UNIVERSITY
"I used it for TOPO cloning, worked well!"
—Daad Abi-Ghanem, IMMUNOLOGY CONSULTANTS LABORATORY INC
"We were very pleased to find that it was the only enzyme that provided robust amplification of single genes housed on single copy number plasmids. We didn't need to use very much template at all to do this reaction which was even better since our templte was from a whole genomic DNA preparation that does not enrich for the plasmids."
—Viveka Vadyvaloo, WASHINGTON STATE UNIVERSITY
"In order to make an overexpressing plasmid, I used PrimeScript 1st Strand cDNA Synthesis kit and Takara Ex Taq DNA Polymerase Hot Start Version. Cloning was perfect. They get easier to make plasmids."
—Koji Ueno, UNIV OF CALIF SAN FRANCISCO
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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