- Ideal for high-fidelity, high-yield endpoint PCR
Nicking activity, endonuclease, and exonuclease activity are not detected after incubation of 0.6 µg of supercoiled pBR322 DNA, 0.6 mg of lambda DNA, or 0.6 µg of lambda-HindIII digest with 10 units of this enzyme for 1 hour at 74°C.
Most PCR products (approximately 80%) amplified with Takara Ex Taq have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.
PCR performance test
PCR performance is confirmed by using lambda DNA as the template (amplified fragment: 20 kb).
PCR performance is also confirmed via amplification of the beta-globin gene from human DNA template (amplified fragment: 17.5 kb).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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