Titanium Taq DNA Polymerase and premix
Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations.
Titanium Taq is available in several formats:
Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations.
Titanium Taq is available in several formats:
- Individual components—separate tubes of Titanium Taq polymerase mix and buffer (dNTPs not included).
- For a complete system, use the Titanium Taq PCR Kit—includes polymerase mix, PCR buffer, 50X dNTP mix, control DNA template, control primer mix, and PCR-grade water.
- A ready-to-use, lyophilized 2X PCR master mix—High Yield PCR EcoDry Premix for convenient room-temperature set up, transport, and storage.
Overview
- High yield—requires fewer PCR cycles, therefore generating less background signal
- Active over wide ranges of Mg2+ concentrations—minimizes time spent on optimization
- Specially engineered DNA polymerase—amplifies highly complex templates up to 2 kb, such as mammalian genomic DNA. Also suitable for rare or low-copy targets
- Integrated hot-start antibody for enhanced specificity—minimizes primer-dimer formation and reduces background, making it suitable for multiplex PCR. Also allows for convenient reaction setup at room temperature
- Lyophilized format available as EcoDry Premix—stores conveniently at room temperature. Ideal for field work or point-of-care assays
More Information
Applications
- Multiplex PCR
- Genotyping
- Hot-start PCR
- Whole-genome PCR
- Rare-template amplification
- Standard PCR, such as genotyping, primer extension
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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2.11 | Protecting the food supply with PCR
Corn crops are vulnerable to a serious bacterial disease known as Stewart's Wilt, which can sharply reduce crop yields. Accurate identification of this pathogen in infected corn plants is critical to minimizing its impact. A team of scientists discovered that Titanium Taq DNA Polymerase was able to generate reproducible PCR banding patterns to distinguish this pathogen from several related strains—providing a rapid, reliable genotyping method to help protect corn crops from a devastating blight. That's Good Science!
Tech notes
Titanium Taq outperforms other polymerases in high-throughput genotyping
Titanium Taq DNA Polymerase is unrivaled for multiplex PCR in high-throughput genotyping applications.
Learn more about whole-genome SNP genotyping with Titanium Taq
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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