TaKaRa Taq DNA Polymerase and premix
TaKaRa Taq DNA Polymerase is a recombinant-version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.
For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).
TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.
For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).
Two other formulations of TaKaRa Taq DNA polymerase are also available:
- Several antibody-mediated hot-start versions of Takara Taq—for preventing non-specific amplification from mispriming during reaction setup
- GMP-grade version—for an added level of confidence in our quality polymerase
Overview
- Excellent for standard PCR amplifications
- Low bacterial DNA contamination
- Premix Taq enables easy PCR setup and minimizes pipetting steps
More Information
- Routine PCR
- DNA sequencing
Source
Recombinant E. coli
PCR products
The resulting PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine (A) to the 3' end. It is also possible to clone the PCR product into blunt-end vectors after blunting and phosphorylation of the ends.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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