Takara Taq DNA polymerase, hot start
The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.
Several hot-start versions of Takara Taq are available:
The hot-start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase, thereby preventing DNA synthesis at room temperature. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot-start PCR enzyme prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during PCR assembly.
Several hot-start versions of Takara Taq are available:
- TaKaRa Taq DNA Polymerase Hot Start Version—enzyme, buffer, and dNTPs are supplied as separate components
- Premix Taq DNA Polymerase Hot Start Version—2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer
- TaKaRa Taq HS Perfect Mix—2X hot-start premix optimized for fast reactions. It contains a modified Taq DNA polymerase, which lacks exonuclease activities, and is compatible with a 20 sec/kb extension step
Overview
Integrated hot-start antibody for enhanced specificity: minimizes primer-dimer formation and reduces background, making it suitable for multiplex PCR. The hot start also allows for convenient reaction setup at room temperature.
More Information
- Hot-start PCR
- Multiplex PCR
Storage
–20°C. Avoid repeated freezing and thawing.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Tech note
Highly efficient and specific multiplex PCR using TaKaRa Taq DNA Polymerase Hot Start Version
Takara HS Taq demonstrates superior efficiency and specificity over standard Taq Polymerase in this multiplex PCR application. Using Takara HS Taq results in target amplification efficiencies equivalent to those of separate (single target) amplification reactions.
See what our customers are saying about Takara Taq DNA Polymerase Hot Start Version!
"This is a great product. We love being able to assemble the reactions at room temperature! We also get very little background. Thanks!"
—Gina Riggio, PENN STATE UNIVERSITY
"Product attended expectations. Sales representative is very efficient and prompt to help too!"
—Leticia Soares, UNIVERSITY OF MISSOURI-SAINT LOUIS
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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