SuperPlex Premix PCR master mix designed for fast, uniform multiplexing

SuperPlex Premix: 2X master mix, ideal for multiplexing

SuperPlex Premix is a 2X, hot-start PCR master mix formulated with our high-yield Titanium Taq DNA polymerase. SuperPlex Premix is ideal for multiplex PCR where unbiased amplification of different targets and high amplicon yield are important. The premix formulation minimizes pipetting steps and streamlines workflows.

Cat. #	Product	Size	Price
638543	SuperPlex™ Premix	200 Rxns	USD $389.00
SuperPlex Premix is a hot-start 2X PCR master mix formulated based on Takara Bio's high-yield Titanium Taq DNA polymerase. SuperPlex Premix is ideal for multiplex PCR as well as high-yield singleplex PCR. The premix contains all the reagents necessary for multiplex PCR and is easy to use—just add primers, sample, and water. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes. We compared the performance of SuperPlex Premix to four other multiplex PCR master mixes using the recommended SuperPlex Premix PCR cycle. Each lane represents the PCR products from a 20-plex reaction obtained from a different premix, visualized with ethidium bromide staining. Lane 1: SuperPlex Premix. Lane 2: KAPA2G Fast Multiplex Mix. Lane 3: Platinum Multiplex PCR Master Mix. Lane 4: NEB Multiplex PCR 5X Master Mix. Lane 5: Qiagen Multiplex PCR Kit, without Q-solution. Lane 6: Qiagen Multiplex PCR Kit, with Q-solution. Back 638543: SuperPlex Premix
638544	SuperPlex™ Premix	1,000 Rxns	USD $1542.00
SuperPlex Premix is a hot-start 2X PCR master mix formulated based on Takara Bio's high-yield Titanium Taq DNA polymerase. SuperPlex Premix is ideal for multiplex PCR as well as high-yield singleplex PCR. The premix contains all the reagents necessary for multiplex PCR and is easy to use—just add primers, sample, and water. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes. We compared the performance of SuperPlex Premix to four other multiplex PCR master mixes using the recommended SuperPlex Premix PCR cycle. Each lane represents the PCR products from a 20-plex reaction obtained from a different premix, visualized with ethidium bromide staining. Lane 1: SuperPlex Premix. Lane 2: KAPA2G Fast Multiplex Mix. Lane 3: Platinum Multiplex PCR Master Mix. Lane 4: NEB Multiplex PCR 5X Master Mix. Lane 5: Qiagen Multiplex PCR Kit, without Q-solution. Lane 6: Qiagen Multiplex PCR Kit, with Q-solution. Back 638544: SuperPlex Premix

Overview

Formulated to minimize the time required to optimize multiplex reactions
High-yield enzyme requires fewer PCR cycles and generates low background signal
Convenient premix formulation simplifies your workflow, saving time and reducing potential errors

More Information

Applications of multiplex PCR

Pathogen detection/identification
High-throughput SNP genotyping
Mutation analysis
Gene deletion analysis
Template quantification

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

SuperPlex Premix: a convenient, high-performing master mix for rapid multiplex PCR results

Multiplex PCR performance comparison: more rapid and more uniform results with SuperPlex Premix than with KAPA, Invitrogen, NEB, or Qiagen premixes.

Review the data

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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SuperPlex Premix PCR master mix designed for fast, uniform multiplexing

Notice to purchaser

Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes

638543: SuperPlex Premix