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  • EmeraldAmp GT PCR Master Mix
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EmeraldAmp MAX PCR Master Mix
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Home › Products › PCR › PCR master mixes › Dye-added › EmeraldAmp GT PCR Master Mix

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High-throughput PCR: EmeraldAmp GT PCR Master Mix

EmeraldAmp GT PCR Master Mix

EmeraldAmp GT PCR Master Mix is a loading-dye-added version of EmeraldAmp MAX PCR Master Mix that is optimized for great performance and convenience in both standard and high-throughput PCR applications. This master mix includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green), and a density reagent in a 2X premix format. Simply add primers and DNA template. Following PCR, amplicons can be used directly in several ways.

EmeraldAmp GT PCR Master Mix is a loading-dye-added version of EmeraldAmp MAX PCR Master Mix that is optimized for great performance and convenience in both standard and high-throughput PCR applications. This master mix includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green), and a density reagent in a 2X premix format. Simply add primers and DNA template. Following PCR, amplicons can be used directly in several ways.

PCR tube contents can be loaded directly onto an agarose gel for electrophoresis or used directly in downstream applications such as restriction enzyme digestion, TA cloning, and direct sequencing. EmeraldAmp GT PCR Master Mix can be used to amplify genomic targets up to ~5 kb and is compatible with GC- and AT-rich targets.

When 5 µl of the loading-dye-added PCR master mix is used for electrophoresis on an 1% Agarose L03 gel, the blue dye front is detected around 3–5 kb, and the yellow dye is detected below 50 bp. These dyes have absorptions at approximately 260 nm and 420 nm, respectively. The dyes can be removed by gel purification or PCR cleanup using the NucleoSpin Gel and PCR Clean-Up kit (where available), if necessary.

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Cat. # Product Size Price License Quantity Details
RR310B EmeraldAmp® GT PCR Master Mix 800 Rxns USD $280.00

This 2X PCR master mix is ideal for high-throughput screening. This convenient premix includes a green gel-loading dye and a density reagent. Reactions performed with this master mix can be loaded directly onto an agarose gel for electrophoresis. Cat. # RR310B contains 5 of Cat. # RR310A. Please refer to Cat. # RR310A for complete product documentation and resources.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR310A EmeraldAmp® GT PCR Master Mix 160 Rxns USD $64.00

This 2X PCR master mix is ideal for high-throughput screening. This convenient premix includes a green gel-loading dye and a density reagent. Reactions performed with this master mix can be loaded directly onto an agarose gel for electrophoresis.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR310A: EmeraldAmp GT PCR Master Mix

RR310A: EmeraldAmp GT PCR Master Mix

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • Convenient premix format—just add template, primers, and water to the 2X PCR master mix
  • Improved performance—includes an optimized buffer for better amplification of GC- and AT-rich targets
  • Eliminates purification steps—PCR contents can be loaded directly onto an agarose gel
  • High-throughput PCR—use reactions directly in TA cloning, restriction enzyme digestion, or sequencing 
  • Green loading dye—allows tracking during gel electrophoresis

More Information

Applications

  • High-throughput PCR

PCR products

Most PCR products amplified with EmeraldAmp GT have a 3'-terminal adenosine (A), and therefore PCR products can be used directly for cloning into T-vectors (TA cloning). It is possible to clone the product into blunt-end vectors after blunting and phosphorylation.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


See what our customers are saying about EmeraldAmp GT PCR Master Mix!

"it is very easy to work with."
—Xiaoyuan Xie, TEXAS A&M UNIVERSITY COLLEGE STATION
"Made great improvements on what has been a difficult process for mouse colony genotyping."
—Jay Phillips, OREGON HEALTH & SCI UNIVERSITY

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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