EmeraldAmp MAX HS PCR Master Mix
EmeraldAmp MAX HS PCR Master Mix is the antibody-mediated hot-start (HS) version of EmeraldAmp MAX PCR Master Mix. The inclusion of an antibody prevents mispriming and non-specific amplification during PCR setup.
EmeraldAmp Max HS PCR Master Mix can be used for routine PCR applications, and completed reactions can be analyzed directly via gel electrophoresis.
EmeraldAmp MAX HS PCR Master Mix is the antibody-mediated hot-start (HS) version of EmeraldAmp MAX PCR Master Mix. The inclusion of an antibody prevents mispriming and non-specific amplification during PCR setup.
EmeraldAmp Max HS PCR Master Mix can be used for routine PCR applications, and completed reactions can be analyzed directly via gel electrophoresis. This hot-start master mix includes a high-yield HS PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (green), and a density reagent in a 2X Taq PCR master mix format. Only primers and DNA template need to be added to initiate the reaction.
Overview
- Antibody-mediated hot start—minimizes nonspecific amplification and primer dimers
- High yield—at least 10 times more end product than conventional PCR
- Excellent performance—optimized buffer for better performance with AT- or GC-rich targets
- Convenience—simply add template, primers, and water to the 2X Taq PCR Master Mix, then load the reactions directly onto a gel upon completion of PCR
- Emerald green loading dye—easy tracking during electrophoresis
- Easy integration with downstream applications—restriction enzyme digests can be performed directly in the PCR buffer
More Information
Applications
- TA cloning or sequencing
- Direct gel electrophoresis
- Long and high-yield PCR
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
See what our customers are saying about EmeraldAmp MAX HS PCR Master Mix!
The reagent worked beautifully. I will order some in the near future for our PCR."
—Jiafen Hu, PENN STATE UNIVERSITY
...for genotyping, better than NEB Taq mix."
—Yaning Li, STANFORD UNIVERSITY
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.