SapphireAmp fast PCR—hot-start master mix
SapphireAmp Fast PCR Master Mix is designed for fast, streamlined PCR, and is, therefore, ideal for applications such as colony PCR. It contains a hot-start PCR enzyme, optimized buffer, dNTPs, along with a blue gel-loading dye and density reagent, in a convenient 2X premix format.
Reactions are assembled by simply mixing primers and DNA template with SapphireAmp Fast PCR Master Mix. For rapid and convenient screening, the amplification reactions can be loaded directly onto an agarose gel immediately after amplification without purification, or digested directly with restriction enzymes.
For E. coli-based colony PCR, inserts up to ~5 kb in length are easily screened; colony PCR can be performed in about an hour.
For human genomic DNA, amplification of 2-kb targets can be completed in one hour; amplifications of up to 6 kb are possible.
- Hot-start PCR master mix is optimized for fast PCR
- Colony PCR can be completed in half the time required for conventional Taq DNA polymerase
- Convenient protocol eliminates purification steps with no reduction in yield
- Restriction enzyme digestion of the PCR products can be performed directly in the PCR buffer
- Reactions can be directly loaded onto a gel without purification or the addition of other reagents
- Hot-start PCR
- Colony PCR
- Fast PCR
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development atto discuss your needs or visit our to submit an inquiry.
Additional product information
Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find high-quality solutions for applications downstream of your PCR
Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.
Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.Clean it Clone it
Find answers to your PCR questions
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.