SapphireAmp fast PCR—hot-start master mix
SapphireAmp Fast PCR Master Mix is designed for fast, streamlined PCR, and is, therefore, ideal for applications such as colony PCR. It contains a hot-start PCR enzyme, optimized buffer, dNTPs, along with a blue gel-loading dye and density reagent, in a convenient 2X premix format.
Reactions are assembled by simply mixing primers and DNA template with SapphireAmp Fast PCR Master Mix. For rapid and convenient screening, the amplification reactions can be loaded directly onto an agarose gel immediately after amplification without purification, or digested directly with restriction enzymes.
For E. coli-based colony PCR, inserts up to ~5 kb in length are easily screened; colony PCR can be performed in about an hour.
For human genomic DNA, amplification of 2-kb targets can be completed in one hour; amplifications of up to 6 kb are possible.
Overview
- Hot-start PCR master mix is optimized for fast PCR
- Colony PCR can be completed in half the time required for conventional Taq DNA polymerase
- Convenient protocol eliminates purification steps with no reduction in yield
- Restriction enzyme digestion of the PCR products can be performed directly in the PCR buffer
- Reactions can be directly loaded onto a gel without purification or the addition of other reagents
More Information
Applications
- Hot-start PCR
- Colony PCR
- Fast PCR
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product′s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Colony PCR: Protocol complete in under an hour
Learn how to speed up your colony PCR with SapphireAmp Fast PCR Master Mix.
Find high-quality solutions for applications downstream of your PCR
Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.
Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.
Clean itFind answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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