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CloneAmp HiFi PCR Premix—high-fidelity PCR for cloning

CloneAmp HiFi PCR Premix is designed for use with the In-Fusion Cloning system due to its exceptionally accurate and efficient DNA amplification. The 2X master mix contains enzyme, optimized buffer, and dNTPs, allowing rapid setup of PCR reactions and facilitating high-throughput applications for multiple cloning samples.

Cat. #	Product	Size	Price	License	Quantity	Details
639298	CloneAmp™ HiFi PCR Premix	200 Rxns	USD $260.00
A convenient 2X PCR master mix that is optimized for high-fidelity and fast PCR. It is designed for use with Takara's In-Fusion Cloning. CloneAmp HiFi PCR Premix is suitable for high-throughput cloning applications. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes Mutation frequency of CloneAmp HiFi Polymerase compared to other high-fidelity PCR enzymes. Eight arbitrarily selected GC-rich regions were amplified with CloneAmp HiFi Polymerase or other DNA polymerases using a Thermus thermophilus HB8 genomic DNA template, and cloned into suitable plasmids. Multiple clones were selected for each amplification product and subjected to sequence analysis. DNA fragments amplified using CloneAmp HiFi Polymerase yielded only 12 mismatched bases per 542,580 total bases—lower than an alternative high-fidelity enzyme from Company A, and 10-fold lower than Taq DNA polymerase. Back 639298: CloneAmp HiFi PCR Premix

Overview

Highly accurate—exceptionally low error rate (12 mismatched bases per 542,580 total bases)
Fast—quick priming and extension (5 sec/kb) enable fast PCR reactions
Specific—hot-start antibody prevents nonspecific amplification
Versatile—works with long (up to 10 kb) and GC-rich templates
Convenient—premix allows rapid reaction setup

More Information

Applications

In-Fusion Cloning for directional, seamless cloning of any PCR fragment or multiple fragments

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

See what our customers are saying about CloneAmp HiFi PCR Premix!

"very good results fast set-up"
—Joanne Pazik, UNIV AT BUFFALO

"We love this new product. It saves time and effort because all the necessary components are included."
—Elena Cardenas Galindo, UNIV OF CALIF SAN DIEGO SCIQUEST

Find high-quality solutions for applications downstream of your PCR

Looking for quick and dependable purification of your amplicon? Easily clean up your PCR product, either from an agarose gel or directly from the reaction.

Plan on using your amplicon for cloning? Seamlessly insert your PCR product into any vector, at any site of linearization using ligation-independent cloning.

Clean it

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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