Random Primer DNA Labeling Kit Ver. 2

The Random Primer DNA Labeling Kit Ver. 2 is designed for radioactive labeling for hybridization probes. Probe labeling can label DNA with [³²P]-alpha-, [³⁵S]-alpha-, or [³H]-alpha-dCTP. This random primer DNA labeling kit is based on a modified method by Feinberg and Vogelstein (Feinberg and Vogelstein 1983; Feinberg and Vogelstein 1984), and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I, Klenow fragment. Using primers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This probe labeling method overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 ng to 20 ng).

Cat. #	Product	Size		License	Quantity	Details
6045	Random Primer DNA Labeling Kit Ver. 2	30 Rxns	USD $185.00
The Random Primer DNA Labeling Kit, Version 2 is designed to yield DNA probes with high activity for hybridization and can be used to label DNA with [³²P]-α-, [³⁵S]-α- or [³H]-α-dCTP. This kit is based on a modified method of Feinberg and Vogelstein that utilizes random oligonucleotide primers and the cloned exonuclease-free Klenow fragment of E. coli DNA polymerase I. The use of longer 9-mer primers and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This method overcomes many of the disadvantages of the conventional nick translation procedure while producing probes from small amounts of DNA (10 to 20 ng). It can also be used to label DNA fragments that are embedded in low-melting temperature agarose gel slices. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Resources Back 6045: Random Primer DNA Labeling Kit Ver. 2

More Information

Applications

Probe labeling
Random primer DNA labeling

References

Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13 (1983).

Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137, 266–7 (1984).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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