BcaBEST Labeling Kit

The BcaBEST DNA labeling kit is a novel random-primer labeling system that uses longer primers (9-mers) and BcaBEST polymerase, a thermostable DNA polymerase isolated from Bacillus caldotenax. Unlike the E. coli Klenow fragment (Feinberg and Vogelstein 1983; Feinberg and Vogelstein 1984) or modified T7 DNA polymerase, Bca polymerase allows stable polymerization of DNA even for templates with high GC content containing highly structured regions. Longer 9-mer primers increase the incorporation efficiency of labeled nucleotides up to 80%.

With this kit, random-primer labeling via thermostable Bca polymerase is accomplished in 10 minutes, resulting in high yields of labeled DNA probe. The kit is designed for use with [³²P]-alpha-, [³⁵S]-alpha-, or [³H]-alpha-dCTP labels, but it can also be used with nonradioactive biotin, digoxigenin, or DNP-labeled dUTPs through dNTP replacement. In general, probes with specific activities of >10⁹ dpm/mg DNA are obtained with [³²P]-alpha-dCTP (~3,000 Ci/mmol, 111 TBq/mmol).

Note: This kit was previously named the "Ladderman Labeling Kit". The kit components have not changed.

Cat. #	Product	Size	Price	License	Quantity	Details
6046	BcaBEST™ Labeling Kit	40 Rxns	USD $228.00
The BcaBEST Labeling Kit is a novel random primer labeling system that takes advantage of longer primers and a thermostable DNA polymerase isolated from Bacillus caldotenax YT-G (Bca DNA polymerase). Unlike the E. coli Klenow fragment or modified T7 DNA polymerase, thermostable Bca DNA polymerase allows stable polymerization through templates with high GC content (highly structured regions). The longer 9-mer primers increase the efficiency of incorporation of labeled nucleotides up to 80%. With this kit, random priming is accomplished within 10 minutes to create efficiently-labeled DNA probes. The kit is designed for use with [³²P]-α-, [³⁵S]-α-, or [³H]-α-dCTP labels, but it can also be used with biotin, digoxigenin or DNP-labeled dUTPs by replacement of the dNTP mixture. Generally, a probe with specific activity of >10⁹ dpm/mg DNA will be obtained with [³²P]-α-dCTP (~3,000 Ci/mmol, 111 TBq/mmol). Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back 6046: BcaBEST Labeling Kit

Overview

Random-primer labeling in 10 minutes
Improved incorporation efficiency especially for templates with complex secondary structure

More Information

Applications

Random-primer labeling of DNA using thermostable Bca polymerase

References

Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13 (1983).

Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137, 266–7 (1984).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

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