Advantage HD Polymerase Mix
The Advantage HD Polymerase Mix provides maximum fidelity and extended PCR product length, making it particularly useful for applications that require high accuracy, such as cDNA cloning, site-directed mutagenesis, and mutation genotyping (i.e., SNP analysis).
The Advantage HD Polymerase Mix consists of a novel DNA polymerase with a hot-start antibody, and comes with a tube of 5X buffer (with Mg2+).
The Advantage HD Polymerase Mix provides maximum fidelity and extended PCR product length, making it particularly useful for applications that require high accuracy, such as cDNA cloning, site-directed mutagenesis, and mutation genotyping (i.e., SNP analysis).
The Advantage HD Polymerase Mix consists of a novel DNA polymerase with a hot-start antibody, and comes with a tube of 5X buffer (with Mg2+).
Advantage HD Polymerase Mix provides outstanding accuracy due to the presence of 3' → 5' exonuclease activity that results in an extremely low error rate. It also performs extremely well on GC-rich and other more complex templates. The Advantage HD Polymerase Mix amplifies targets of up to 8.5 kb from human genomic DNA, 10 kb from E. coli genomic DNA, and 22 kb from lambda DNA. The amplification efficiency of Advantage HD Polymerase Mix is greater than that of wild-type Taq polymerase.
Overview
- Extremely low error rate—exonuclease proofreading yields just 12 errors per 250,000 bp, and performs well on GC-rich templates.
- Integrated hot-start antibody for enhanced specificity—prevents false initiation during reaction setup and minimizes primer-dimer formation, thus reducing background.
- High priming efficiency—shortens reaction time and produces results fast.
- Direct cloning—PCR products can be directly cloned into blunt-end vectors. If primers having sufficient vector sequence homology are used, then products can be cloned directly using our In-Fusion Cloning technology.
More Information
Applications
- In-Fusion Cloning for directional, seamless cloning of any PCR fragment or multiple fragments to general page
- Blunt-end cloning
- cDNA cloning
- Site-directed mutagenesis
- Mutation genotyping (SNP analysis)
PCR products
All of the PCR products obtained using Advantage HD Polymerase Mix will possess blunt ends due to the 3' → 5' exonuclease activity of the enzyme. Consequently, the PCR products can be directly cloned into blunt-end vectors. If primers having sufficient vector sequence homology are used, then products can be cloned directly using In-Fusion technology.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
See what our customers are saying about Advantage HD Polymerase Mix!
This product amplifies very robustly."
—Carla Hurt, TENNESSEE TECHNOLOGICAL UNIVERSITY
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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