LA Taq PCR kit

The LA PCR Kit, Version 2.1 contains all the reagents needed for amplification of long DNA templates, enabling routine amplification of products up to 20 kb in length, including GC-rich amplicons. For some DNA templates, longer amplification is possible. This long-range PCR kit contains TaKaRa LA Taq DNA Polymerase, buffers, MgCl2, dNTPs, molecular weight markers, and control templates, plus corresponding primers to ensure optimal PCR performance during long PCR.  

Overview

  • Genomic DNA template amplification, with included control reactions
  • Lambda DNA template amplification, with included control reactions
  • Longer and more accurate PCR products
  • Specialized 2X GC buffer provided for templates with high GC content or extensive secondary structure

More Information

PCR products

PCR products generated with LA Taq DNA Polymerase contain a mixture of 3'-A overhangs and blunt ends, resulting in >80% cloning efficiency in T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.