LA Taq DNA polymerase, hot-start version
TaKaRa LA Taq DNA Polymerase Hot-Start Version consists of Takara LA Taq polymerase plus a monoclonal antibody. The antibody binds Taq polymerase, thereby preventing nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly. This hot-start version of LA Taq retains all of the high-performance features of Takara LA Taq polymerase while increasing reaction specificity and reducing background. An optimized LA PCR Buffer II (Mg2+) and dNTP mixture are supplied with the polymerase.
TaKaRa LA Taq DNA Polymerase Hot-Start Version consists of Takara LA Taq polymerase plus a monoclonal antibody. The antibody binds Taq polymerase, thereby preventing nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly. This hot-start version of LA Taq retains all of the high-performance features of Takara LA Taq polymerase while increasing reaction specificity and reducing background. An optimized LA PCR Buffer II (Mg2+) and dNTP mixture are supplied with the polymerase.
For hot-start long-range PCR of targets with high GC content in high-fidelity applications, we recommend using PrimeSTAR GXL DNA Polymerase (Cat. # R050A).
Use our LA PCR Genome DNA Set as your long-range PCR experimental controls. The set includes purified, high-molecular-weight human and E. coli genomic DNA plus appropriate primers.
For non-hot-start options, visit our LA Taq DNA polymerase page to learn about the different formulations we offer.
Overview
- Robust amplification of long DNA templates (long-range PCR)—products up to 48 kb are possible
- Integrated antibody-mediated hot-start PCR—reduces non-sepcific background amplification and increases reaction specificity
- Specially optimized buffer (LA Buffer II)—less optimization is required and gives greater yield
- DNA proofreading polymerase mix—offers higher fidelity compared with conventional Taq DNA polymerase
More Information
Applications
- Long-range PCR to amplify products up to 48 kb
- Amplification of highly homologous sequences
- Pseudogene identification
- Mitochondrial DNA sequencing
PCR products
PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Long-range PCR with Takara LA Taq DNA polymerase
Examples of long-range PCR using Takara LA Taq with E. coli or human genomic DNA templates.
See what our customers are saying about Takara LA Taq DNA Polymerase!
"It works very well for long pcr and easy optimise conditions. I have used it extensively in amplifiying 4kb to 17kb."
—Praveen Hajeri, UNIVERSITY OF MINNESOTA
"I have purchased your LA Taq. I tried it with and without adding pfu. I was looking for more product per PCR reaction. I got ~2X the amount vs my old Taq in a side by side test. Thanks "
—Maureen Tarsio, RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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