One-Shot LA PCR Mix
One Shot LA PCR Mix Ver. 2.0 enables long-range PCR in a convenient premix format. This long and accurate PCR enzyme premix is composed of LA Taq DNA polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25-µl aliquots predispensed into 0.2-ml PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each tube to obtain a complete and ready-to-amplify reaction.
One Shot LA PCR Mix Ver. 2.0 enables long-range PCR in a convenient premix format. This long-and-accurate PCR enzyme premix is composed of LA Taq DNA Polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25-µl aliquots predispensed into 0.2-ml PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each tube to obtain a complete and ready-to-amplify reaction.
LA Taq is also offered in the following formats:
- Individual components—LA Taq DNA polymerase supplied with a tube of optimized LA PCR Buffer II (without Mg2+), MgCl2, and dNTPs
- TaKaRa LA Taq DNA Polymerase Hot-Start Version—antibody-meditated hot-start PCR to increase reaction specificity and reduce background
- LA PCR Kit Ver. 2.1—includes all reagents plus controls needed for amplification of long DNA templates
Use our LA PCR Genome DNA Set as your long range PCR experimental controls. The set includes purified, high molecular weight human and E. coli genomic DNA plus appropriate primers.
Overview
- Convenient and reliable long-range PCR
- Longer and more accurate genomic PCR amplifications
- Increased reproducibility and reduced optimization for long PCR
More Information
PCR products
PCR products generated with LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the cloning efficiency of longer inserts (>5 kb) into T-vectors is quite low.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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