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  • ‹ Back to LA Taq products
  • LA Taq DNA polymerase
  • LA Taq DNA polymerase HS
  • LA Taq PCR kit
  • LA Taq premix
Amplification of human genomic DNA by Takara LA Taq DNA polymerase LA Taq
long distance pcr Hot-start LA Taq
Long PCR controls Long PCR controls
Tips and FAQs PCR FAQs
Home › Products › PCR › Long-range PCR › LA Taq products › LA Taq premix

Long-range PCR

  • LA Taq products
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    • LA Taq DNA polymerase HS
    • LA Taq PCR kit
    • LA Taq premix
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Amplification of human genomic DNA by Takara LA Taq DNA polymerase LA Taq
long distance pcr Hot-start LA Taq
Long PCR controls Long PCR controls
Tips and FAQs PCR FAQs

One-Shot LA PCR Mix

One Shot LA PCR Mix Ver. 2.0 enables long-range PCR in a convenient premix format. This long and accurate PCR enzyme premix is composed of LA Taq DNA polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25-µl aliquots predispensed into 0.2-ml PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each tube to obtain a complete and ready-to-amplify reaction.

One Shot LA PCR Mix Ver. 2.0 enables long-range PCR in a convenient premix format. This long-and-accurate PCR enzyme premix is composed of LA Taq DNA Polymerase, LA PCR Buffer II, and dNTPs provided at 2X concentration in 25-µl aliquots predispensed into 0.2-ml PCR tubes. Simply add an equal volume of solution containing DNA template and primers to each tube to obtain a complete and ready-to-amplify reaction.

LA Taq is also offered in the following formats:

  • Individual components—LA Taq DNA polymerase supplied with a tube of optimized LA PCR Buffer II (without Mg2+), MgCl2, and dNTPs
  • TaKaRa LA Taq DNA Polymerase Hot-Start Version—antibody-meditated hot-start PCR to increase reaction specificity and reduce background
  • LA PCR Kit Ver. 2.1—includes all reagents plus controls needed for amplification of long DNA templates

Use our LA PCR Genome DNA Set as your long range PCR experimental controls. The set includes purified, high molecular weight human and E. coli genomic DNA plus appropriate primers.

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Cat. # Product Size License Quantity Details
RR004 One Shot LA PCR™ Mix Ver. 2.0 1 Set USD $160.00

A 2X PCR master mix in 25 μl-aliquots in 0.2 ml PCR tubes. The master mix contains Takara LA Taq and all reagents needed for PCR reactions. Simply add template and primers to each tube to begin your PCR. 

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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RR004: One Shot LA PCR Mix Ver. 2.0

RR004: One Shot LA PCR Mix Ver. 2.0

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • Convenient and reliable long-range PCR
  • Longer and more accurate genomic PCR amplifications
  • Increased reproducibility and reduced optimization for long PCR

More Information

PCR products

PCR products generated with LA Taq contain a mixture of 3'-A overhangs and blunt ends, allowing >80% cloning efficiency into T-vectors. However, the cloning efficiency of longer inserts (>5 kb) into T-vectors is quite low.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


pcr success PCR learning center
Citations Citations: LA Taq

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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  • New products
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  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Reproductive health
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Diagnostic solutions
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  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
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  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
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  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • Special offers
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  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
  • NGS bundle and save
  • Free sample: PrimePath Direct Saliva SARS-CoV-2 Detection Kit
  • TALON his-tag purification resin special offer
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  • Identification and characterization
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  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
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