LA Taq DNA polymerase with GC buffers
TaKaRa LA Taq DNA Polymerase with GC Buffer has the Takara LA Taq DNA Polymerase blend for long and accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. The GC-optimized Buffers I and II are specifically designed to amplify GC-rich DNA templates (up to 73% GC content) or templates with a significant amount of secondary structure:
TaKaRa LA Taq DNA Polymerase with GC Buffer has the TaKaRa LA Taq DNA Polymerase blend for long-and-accurate PCR paired with GC-optimized buffers to power through difficult-to-amplify GC-rich sequences, including genomic DNA. The GC-optimized Buffers I and II are specifically designed to amplify GC-rich DNA templates (up to 73% GC content) or templates with a significant amount of secondary structure:
- GC Buffer I is recommended for amplification of fragments that are ≥5 kb in length
- GC Buffer II is recommended for fragments that are 2–3 kb in length
For the longest amplicons that are also GC rich, try PrimeSTAR GXL DNA Polymerase, which is designed to amplify genomic DNA up to 30 kb.
Overview
- Long and accurate PCR amplification of genomic DNA targets (long-range PCR)
- Optimized buffers (GC Buffers I & II) for amplification of templates with high GC content and/or significant secondary structure
- High-yield amplification of long DNA targets with minimal optimization
More Information
PCR products
PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low. It is also possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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How the leopard lost its spots
Why do the offspring of some leopards hide their spots, resulting in black panthers? An international team of researchers focused on ASIP, a gene that causes dark coat coloration in other felines. Takara Bio's LA Taq with GC Buffer was used to sequence a tricky stretch of exon 4 in leopards and Asian golden cats, identifying SNPs associated with melanistic coat color. Scientists can now answer the curious question of how leopards produce panthers. That's Good Science!
Featured product: Takara LA Taq DNA Polymerase paired with GC-optimized buffers
See what our customers are saying about Takara LA Taq DNA Polymerase!
"It works very well for long pcr and easy optimise conditions. I have used it extensively in amplifiying 4kb to 17kb."
—Praveen Hajeri, UNIVERSITY OF MINNESOTA
"I have purchased your LA Taq. I tried it with and without adding pfu. I was looking for more product per PCR reaction. I got ~2X the amount vs my old Taq in a side by side test. Thanks "
—Maureen Tarsio, RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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