PCR products generated with Takara LA Taq contain a mixture of 3'-A overhangs and blunt ends, which allows >80% cloning efficiency into T-vectors. However, the efficiency of cloning longer products (>5 kb) into T-vectors is quite low. It is also possible to clone the product in blunt-end vectors after blunting and phosphorylation of the end.
Performance is confirmed by successful amplification of a 35-kb lambda DNA template, and the β-globin gene (17.5-kb) using human genomic DNA with GC Buffer I. Amplification of the c-jun proto-oncogene (1,255 bp, 65% GC content) by PCR is also confirmed using human genomic DNA as the template with GC Buffer I and II.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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