PrimeSTAR HS with GC buffer
PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content), and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.
PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content), and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.
If you are interested in Commercial Use of our products, including OEM services, bulk purchasing, or custom formulation (e.g., highly concentrated reagents, custom volumes), please contact Corporate Development at bd_oem@takarabio.com to discuss your needs, or visit our OEM page to submit an inquiry.
Overview
- Only 25 errors per 304 kb when using GC-rich template DNA (as determined by sequence analysis)
- Higher efficiency than standard Taq polymerase
- Capable of amplifying high-GC human genomic DNA targets up to 5 kb
- Fast reaction time due to an increased priming efficiency
- High specificity due to antibody-mediated hot-start technology
More Information
Applications
- High-fidelity PCR
- High-GC-content PCR
- Amplification of cDNA libraries
- cDNA cloning for protein expression
Purity
Nicking, endonuclease, nor exonuclease activities are detected upon incubation of 0.6 µg of supercoiled or lambda HindIII-digested pBR322 DNA with 10 units of PrimeSTAR HS enzyme for 1 hour at 74°C.
Storage
–20°C.
Storage Buffer
- [50 mM Tris-HCl (pH 8.2 at 4°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 50% Glycerol]
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2018 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.
