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  • ‹ Back to GC rich PCR
  • PrimeSTAR GXL DNA Polymerase
  • PrimeSTAR HS with GC buffer
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Product page: PrimeSTAR GXL polymerase PrimeSTAR GXL polymerase, research use
Product page: PrimeSTAR HS DNA Polymerase PrimeSTAR HS DNA Polymerase
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Home › Products › PCR › GC rich PCR › PrimeSTAR HS with GC buffer

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Product page: PrimeSTAR HS DNA Polymerase PrimeSTAR HS DNA Polymerase
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PrimeSTAR HS with GC buffer

high-fidelity PCR

PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content) and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.

PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content) and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.

For longer amplicons with high GC or AT content, use PrimeSTAR GXL DNA Polymerase, which is optimized to amplify up to 40 kb from certain targets.

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Cat. # Product Size Price License Quantity Details
R044B PrimeSTAR® HS DNA Polymerase with GC Buffer 1,000 Units USD $698.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

Cat. # R044B is the hot-start PrimeSTAR HS DNA polymerase paired with a specially designed GC buffer (Mg2+ plus). This system is ideal for high-fidelity amplification of high-GC (≥75%) templates. It is suitable for cloning or library construction where high accuracy and high specificiy are required. Cat. # R044B contains 4 of Cat. # R044A. Please refer to Cat. # R044A for complete product documentation and resources.


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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R044A PrimeSTAR® HS DNA Polymerase with GC Buffer 250 Units USD $206.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

Cat. # R044A is the hot-start PrimeSTAR HS DNA polymerase paired with a specially designed GC buffer (Mg2+ plus). This system is ideal for high-fidelity amplification of high-GC (≥75%) templates. It is suitable for cloning or library construction where high accuracy and high specificiy are required.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data Resources

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Application Example

Application Example
Application Example. Comparison of PCR amplification efficiency and specificity between PrimeSTAR HS DNA Polymerase with GC Buffer and high-fidelity/GC-adaptive PCR enzymes from three companies. PrimeSTAR HS DNA Polymerase demonstrated superior efficiency and specificity with a GC-rich template (746 bp of the ApoE gene, with 73.9% GC content) compared to the other enzymes.

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R044A: PrimeSTAR HS DNA Polymerase with GC Buffer

R044A: PrimeSTAR HS DNA Polymerase with GC Buffer

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • Only 25 errors per 304 kb when using GC-rich template DNA (as determined by sequence analysis)
  • Higher efficiency than standard Taq polymerase
  • Capable of amplifying high-GC human genomic DNA targets up to 5 kb
  • Fast reaction time due to an increased priming efficiency
  • High specificity due to antibody-mediated hot-start technology

More Information

Applications

  • High-fidelity PCR
  • High-GC-content PCR
  • Amplification of cDNA libraries
  • cDNA cloning for protein expression

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Primer design

Frequently asked questions about primer design for successful PCR.

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Frequently asked questions about PCR optimization.

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Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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