PrimeSTAR HS with GC buffer
PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content), and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.
PrimeSTAR HS DNA Polymerase with GC Buffer is designed for high-fidelity PCR amplification of GC-rich templates (75% or greater GC content), and is based on our unique, high-fidelity PCR polymerase, PrimeSTAR HS DNA Polymerase. The GC buffer supplied with PrimeSTAR HS facilitates robust, efficient, and accurate extension through even highly GC-rich template regions. It offers both maximal accuracy and better amplification efficiency of high-GC templates than regular Taq polymerase. A dNTP mixture is also included in the kit.
For longer amplicons with high GC or AT content, use PrimeSTAR GXL DNA Polymerase, which is optimized to amplify up to 40 kb from certain targets.
Overview
- Only 25 errors per 304 kb when using GC-rich template DNA (as determined by sequence analysis)
- Higher efficiency than standard Taq polymerase
- Capable of amplifying high-GC human genomic DNA targets up to 5 kb
- Fast reaction time due to an increased priming efficiency
- High specificity due to antibody-mediated hot-start technology
More Information
Applications
- High-fidelity PCR
- High-GC-content PCR
- Amplification of cDNA libraries
- cDNA cloning for protein expression
Purity
Nicking, endonuclease, nor exonuclease activities are detected upon incubation of 0.6 µg of supercoiled or lambda HindIII-digested pBR322 DNA with 10 units of PrimeSTAR HS enzyme for 1 hour at 74°C.
Storage
–20°C.
Storage Buffer
- [50 mM Tris-HCl (pH 8.2 at 4°C), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 50% Glycerol]
Bulk, custom, and OEM information
If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Find answers to your PCR questions
Primer design
Frequently asked questions about primer design for successful PCR.
Optimization
Frequently asked questions about PCR optimization.
Troubleshooting
Frequently asked questions about troubleshooting your PCR problems.
Applications and conditions
Frequently asked questions about general and specific applications for PCR and which polymerases to choose.
Shipping, storage, and handling
Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.
Avoid DNA contamination in PCR
There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.
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