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  • PrimeSTAR HS DNA Polymerase
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Product page: PrimeSTAR HS with GC buffer PrimeSTAR HS with GC buffer
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Product page: PrimeSTAR HS with GC buffer PrimeSTAR HS with GC buffer
Product page: PrimeSTAR GXL polymerase PrimeSTAR GXL polymerase, research use
PrimeSTAR Max DNA Polymerase PrimeSTAR Max DNA Polymerase
Tips and FAQs PCR FAQs

PrimeSTAR HS DNA Polymerase

High-fidelity PCR

PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3' → 5' exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HS is available in flexible and convenient formats:

  • Individual components—PrimeSTAR HS DNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
  • 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.

PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3' → 5' exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HS is available in flexible and convenient formats:

  • Individual Components—PrimeSTAR HS DNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
  • 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.

The fidelity of PrimeSTAR HS DNA Polymerase was calculated by direct sequencing analysis to provide a more realistic estimate of the rate of incorporated errors compared with other commonly used indirect phenotypic analyses such as lacI. Calculation methods such as lacI, which are often used by other enzyme suppliers, are prone to error due to inherent lethal and silent mutations.

PrimeSTAR HS DNA Polymerase also offers excellent amplification efficiency and a fast reaction time due to its extremely high priming efficiency. Highly specific amplifications can be achieved using short annealing times of just 5–15 seconds.

For the most challenging PCR applications, enhanced versions of PrimeSTAR HS are available with the ability to amplify GC-rich sequences easily, even higher fidelity, and for long-range PCR:

  • PrimeSTAR HS with GC buffer provides reliable, high-fidelity amplification for the most difficult GC-rich content (75% or greater).
  • PrimeSTAR Max products provide the highest fidelity and fastest extension time of any Takara Bio product.
  • PrimeSTAR GXL products provide high fidelity plus the ability to amplify long and GC- or AT-rich targets. 
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Cat. # Product Size License Quantity Details
R040A PrimeSTAR® HS DNA Polymerase (premix) 100 Rxns USD $164.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

A 2X hot-start PCR master mix containing Takara's high-fidelity PrimeSTAR HS DNA polymerase, optimized reaction buffer, and dNTPs. PrimeSTAR HS DNA polymerase has superior proofreading ability due to robust 3' to 5' exonuclease activity. It can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA. Please refer to the User Manual for premix composition.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R040A: PrimeSTAR HS DNA Polymerase (premix)

R040A: PrimeSTAR HS DNA Polymerase (premix)
R010B PrimeSTAR® HS DNA Polymerase 1,000 Units USD $733.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

A high-fidelity hot-start (HS) PCR DNA polymerase with superior proofreading ability due to robust 3' to 5' exonuclease activity. PrimeSTAR HS DNA polymerase can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA.  Separate tubes of optimzed buffer (Mg2+ plus) and dNTP mix are supplied with the enzyme. Cat. # R010B contains 4 of Cat. # R010A. Please refer to Cat. # R010A for complete product documentation and resources.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Image Data Resources

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R010B: PrimeSTAR HS DNA Polymerase

R010B: PrimeSTAR HS DNA Polymerase
R010A PrimeSTAR® HS DNA Polymerase 250 Units USD $217.00

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 

A high-fidelity hot-start (HS) PCR DNA polymerase with superior proofreading ability due to robust 3' to 5' exonuclease activity. PrimeSTAR HS DNA polymerase can efficiently amplify up to 8.5 kb for human genomic DNA targets or up to 22 kb for lambda DNA.  Separate tubes of optimzed buffer (Mg2+ plus) and dNTP mix are supplied with the enzyme.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data Resources

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PrimeSTAR High Amplification Efficiency

 PrimeSTAR High Amplification Efficiency
PrimeSTAR High Amplification Efficiency. Amplification efficiency was compared using high fidelity enzymes from Company A and Company B. (Target: Human DCLRE1A gene [2kb]) Reaction mixtures were prepared and PCR cycling conditions were performed according to each company's protocol (50 µL PCR reaction). These results demonstrate that PrimeSTAR provides excellent amplification efficiency with higher specificity than other suppliers' high fidelity enzymes. In addition, the detection sensitivity was higher by one order of magnitude.

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Sequence analysis of amplified DNA fragments

Sequence analysis of amplified DNA fragments
Sequence analysis of amplified DNA fragments. Sequence analysis is the most accurate method to investigate mutation frequency. This analysis revealed that PrimeSTAR HS has higher fidelity than an alternative high fidelity enzyme from Company A, with only 12 mismatched bases per 249,941 total bases, and 10X higher fidelity than Taq DNA polymerase. These results demonstrate PrimeSTAR HS DNA Polymerase's suitability for PCR amplifications that require extreme accuracy.

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R010A: PrimeSTAR HS DNA Polymerase

R010A: PrimeSTAR HS DNA Polymerase

Primer design FAQs Primer design FAQs
Optimization FAQs Optimization FAQs
Troubleshooting FAQs Troubleshooting FAQs

Overview

  • High accuracy and strong exonuclease activity, resulting in an extremely low error rate of only 12 errors per 250 kb
  • Higher amplification efficiency compared with standard Taq polymerase
  • Excellent performance even with GC-rich templates
  • High tolerance to varying reaction conditions (a single PCR cycling protocol can be used to amplify products of varying sizes)
  • Amplification of targets up to 8.5 kb with human genomic DNA, 10 kb with E. coli genomic DNA, and 22 kb with lambda DNA
  • Fast reaction time due to increased priming efficiency
  • Hot-start PCR enzyme prevents false initiation events during reaction assembly due to mispriming or primer digestion

More Information

Applications

  • High-fidelity PCR
  • Amplification of cDNA libraries
  • cDNA cloning for protein expression
  • Site-directed mutagenesis and mutant genotyping (e.g., SNP analysis)

Note regarding fidelity comparison

We use sequencing analysis to determine incorporation error rate because most researchers who require high-fidelity amplification perform the following: PCR, cloning, and sequencing. Determination of error rate by direct sequence analysis, therefore, corresponds closely to the method that most researchers would use in their own applications. Please note that values obtained using different fidelity measurement methods (e.g., direct sequencing, Kunkel method, Cline method, lacI method) do not yield directly comparable data.

PCR products

A significant percentage of PCR products obtained using PrimeSTAR HS DNA polymerase will have blunt ends. As a result, PCR products can be directly cloned into blunt-ended vectors. If necessary, phosphorylate PCR products before cloning.

Bulk, custom, and OEM information

If you are interested in bulk purchasing, custom packaging, custom formulations (including glycerol-free and high concentration), or partnership opportunities, please contact Corporate Development at bd_oem@takarabio.com to discuss your needs or visit our OEM page to submit an inquiry.

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


pcr success PCR learning center
Selection guides PCR selection guide
Citations Citations: PrimeSTAR HS

See what our customers are saying about PrimeSTAR HS DNA Polymerase!

"The activity of primeSTAR HS DNA polymerase is very good. Thank you for your sample! We will use your enzyme and recommend to my colleagues."
—Shigui Zhu, PROTEIN ONE
"This polymerase solved the problems I was having both with PCR and sub-cloning with the products."
—Ethan Duane, UNIV OF NEW HAMPSHIRE

Find answers to your PCR questions

Primer design

Frequently asked questions about primer design for successful PCR.

Optimization

Frequently asked questions about PCR optimization.

Troubleshooting

Frequently asked questions about troubleshooting your PCR problems.

Applications and conditions

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

Shipping, storage, and handling

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

Avoid DNA contamination in PCR

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.

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