TB Green Premix Ex Taq II (Tli RNase H Plus) is ideal for real-time PCR experiments where specificity is critical. A high level of precision is achieved and maintained across samples through effective suppression of amplification of primer dimers and off-target products. This reagent is recommended for situations where primer design has been optimized, but increased qPCR specificity is still needed.
In this study, the mRNA levels of transcription factor E2F1 were analyzed in HEK293 cells transfected with either an experimental or control plasmid. The protocol was carried out with TB Green Premix Ex Taq II (Cat. # RR820Q) on a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific), using a 30-second extension step.