Real-time PCR tutorial videos

Scientists worldwide trust real-time quantitative PCR (RT-qPCR) reagents from Takara Bio, especially for the most demanding RT-qPCR experiments. When you use our sensitive and specific RT-qPCR reagents you can spend less time on RT-qPCR troubleshooting and more time on moving your research forward. We offer RT-qPCR kits for use with both green intercalating dye and probe detection. Compatible with all major RT‑qPCR instrument systems, these products allow you to obtain accurate, consistent results from a wide variety of sample types, even when other reagents fail. Count on us to help you choose the best RT‑qPCR products for plant, soil, blood, paraffin-embedded tissues, archival, or degraded samples or when conducting gene expression studies, forensic research analyses, or other clinical research applications.

Want to learn more about RT-qPCR? These three videos produced by the TATAA Biocenter in Sweden will help you understand everything from the basics of real-time PCR to data analysis techniques (see topic descriptions below the form). Each 20- to 30-minute video can be watched at your convenience from the comfort of your office, lab, or home. Sign up below to watch the recorded webinars.

Part 1: Starting with qPCR

Learn how to:

• Find the right detection method
• Study the expression of your gene
• Check the accuracy of your results

Speaker: Professor Mikael Kubista, TATAA Biocenter

Part 2: How to interpret your RT-qPCR data

Learn how to:

• Determine Cq values
• Obtain absolute quantification of your starting DNA
• Define a good reference for relative quantification
• Follow MIQE guidelines

Speaker: Robert Sjöback, Ph.D., TATAA Biocenter

Part 3: Helping you with your qPCR troubles

Learn how to:

• Identify potential pitfalls in qPCR data
• Detect PCR inhibition
• Control the quality of RNA and reverse transcription

Speaker: Kristina Lind, Ph.D., TATAA Biocenter