Real-time PCR tutorial videos
Scientists worldwide trust real-time quantitative PCR (RT-qPCR) reagents from Takara Bio, especially for the most demanding RT-qPCR experiments. When you use our sensitive and specific RT-qPCR reagents you can spend less time on RT-qPCR troubleshooting and more time on moving your research forward. We offer RT-qPCR kits for use with both green intercalating dye and probe detection. Compatible with all major RT‑qPCR instrument systems, these products allow you to obtain accurate, consistent results from a wide variety of sample types, even when other reagents fail. Count on us to help you choose the best RT‑qPCR products for plant, soil, blood, paraffin-embedded tissues, archival, or degraded samples or when conducting gene expression studies, forensic research analyses, or other clinical research applications.
Want to learn more about RT-qPCR? These three videos produced by the TATAA Biocenter in Sweden will help you understand everything from the basics of real-time PCR to data analysis techniques (see topic descriptions below the form). Each 20- to 30-minute video can be watched at your convenience from the comfort of your office, lab, or home. Sign up below to watch the recorded webinars.
Learn how to:
- Find the right detection method
- Study the expression of your gene
- Check the accuracy of your results
Speaker: Professor Mikael Kubista, TATAA Biocenter
Part 2: How to interpret your RT-qPCR data
Learn how to:
- Determine Cq values
- Obtain absolute quantification of your starting DNA
- Define a good reference for relative quantification
- Follow MIQE guidelines
Speaker: Robert Sjöback, Ph.D., TATAA Biocenter
Part 3: Helping you with your qPCR troubles
Learn how to:
- Identify potential pitfalls in qPCR data
- Detect PCR inhibition
- Control the quality of RNA and reverse transcription
Speaker: Kristina Lind, Ph.D., TATAA Biocenter
High-throughput real-time PCR
Where throughput meets flexibility
Real-time PCR (qPCR) is a powerful technique for genotyping and gene expression analysis. Currently, qPCR experiments are becoming increasingly complex—involving an expansive and growing list of targets from a larger number of samples, all with more technical replicates. The SmartChip Real-Time PCR System is a complete high-throughput solution that enables an unrivaled amount of flexible assay and sample formats, allowing researchers to seamlessly switch between dispensing assay reagents and samples into blank chips, or dispensing samples into custom, preprinted chips without the need for revalidation.Learn more Product details
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