- Gene editing
- Viral transduction
- T-cell transduction and culture
- Inducible systems
- Xfect Transfection Reagent
- Transfection tips
- Cell lines transfected with the Xfect reagent
- Protein transfection FAQs
- Xfect RNA Transfection Reagent
- Transfecting with single shots video
- Single shots picture protocol
- Transfection reagent products
- Protocol: transfection of cerebellar slice cultures with Xfect reagent
- Calcium phosphate transfection of neurons
- Fluorescent proteins
Cell lines transfected with the Xfect reagent
We have satisfied customers who report using our Xfect reagent for the following cells and cell lines. See if your preferred cells have been successfully transfected with the Xfect reagent.
BHK-21, BJ fibroblasts, chick fibroblasts, COS-7, HT1080, mouse embryonic fibroblasts (MEF), NIH3T3, Rat2, swiss mouse embryonic fibroblasts
16HBE, A431, A549, As4.1, B16-F10, BEAS-2B, BSC1, Caco-2, EpH4, H1299, HaCaT, HC11, HEK-293, HEK-293T, Hepa 1-6, hOSE, HT-1080, HT-29, lens epithelial cells (human), MCF7, MDCK, mpkCCD, NMuMG, T-47D, WRL 68
Aortic endothelia (human), HUVEC, myoendothelia (human)
Jurkat, DC2.4, K-562, NALM-6, RAW 264.7, RBL-2H3, SupT1
293FT, As4.1, BHK-21, B-S-C1, COS-7, HEK-293, HEK-293T, mpkCCD, renal proximal tubules (mouse)
16HBE, A549, BEAS-2B, H1299
C2C12, aortic smooth muscle (human), cardiomyocytes (primary rat), myoblasts (primary human), smooth muscle cells (primary human)
SH-SY5Y, HEI-193 (human Schwann), astrocytes (primary mouse)
hADSC (adipose), hMSC (mesenchymal), mesenchymal progenitor, P19
CHO, EpH4, HC11, HeLa, hOSE, LbT2, MCF7, NMuMG, PC3, T-47D
See what our customers are saying about Xfect Transfection Reagent!
"very straight forward!"
—Lu Zhang, UNC CHAPEL HILL
"It is certainly better than 2 transfection reagents we used before from other companies. the result is very good and the price is right."
—Tao Yang, VAN ANDEL INSTITUTE
"Tested on MEFs and it worked great, even for transfecting more than one plasmid. Thanks!!"
—A Clayton, BC CANCER RESEARCH CENTRE
"Easy to use and dependable. Gives reproducible results but has some toxicity."
—Patrick Dolan, PURDUE UNIVERSITY
"I liked that it is easy to use and really gets my hard-to-transfect mouse embryonic fibroblasts transfected."
—Noemi Polgar, UNIV OF HAWAII
"I used it for A549 and H1299 cells, it worked Excellent, very happy with the product."
—Ranadheer Dontula, UNIV OF ILLINOIS
"Similar transfection effeciency and less cell toxity, compared to lipo2000."
—KUN DU, UNIV OF ROCHESTER
"We tried it on RAW 264.7 with about 50% transfection efficiency compared to Lipofectamnie 2000 with only 15% efficiency and a lot of other reagents with even less efficiency (Xtreme gene, TransIT, JetPEI macrophage). We already purchased the product for use in our lab as the best method for transfection of RAW cells that we tried so far."
—Franziska Moll, UNIVERSITY OF HAWAII
"It works well."
—Bingbing Li, OREGON HEALTH & SCI UNIVERSITY
"It transfected just as well as the other transfection reagent I was using and the cells seemed to be healthier overall."
—Koy Saeteurn, UNIV OF CALIF SAN FRANCISCO
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2020 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.