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  • ‹ Back to Tet-inducible systems
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Home › Learning centers › Gene function › Inducible systems › Tet-inducible systems › Tet-On 3G plasmid system kit components

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Tet-On 3G inducible expression plasmid systems—kit components

All plasmid-based versions of the Tet-On 3G System contain the following seven components:

  • 10 μg regulator plasmid (see tables below)
  • 10 μg response plasmid (see tables below)
  • 10 μg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or pTRE-BI-Luc)
  • 2 μg Linear Hygromycin Marker (also sold separately as Cat. # 631625)
  • 2 μg Linear Puromycin Marker (also sold separately as Cat. # 631626)
  • 100 rxns Xfect transfection reagent (also sold separately as Cat. # 631317)
  • 50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. # 631105)

Use the tables below to pick the specific vectors and system that meet your needs. If you prefer a system that uses the human EF1-alpha promoter to express the transactivator protein, please refer to the second table below. EF1-alpha systems are recommended for use in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. Note that lentiviral, retroviral, and adenoviral Tet-On 3G inducible expression systems are also available.

Tet-On 3G Inducible Expression Systems with CMV Regulator Vectors
Cat. #
ProductRegulator vectorResponse vector
631168

Tet-On 3G Inducible Expression System

 

 

 

pCMV-Tet3G regulator plasmid

pTRE3G response plasmid
631337

Tet-On 3G Bidirectional Inducible Expression System

pTRE3G-BI bidirectional response plasmid
631166

Tet-On 3G Inducible Expression System (Bicistronic Version)

pTRE3G-IRES bicistronic response plasmid
631338

Tet-On 3G Bidirectional Inducible Expression System (with mCherry)

pTRE3G-BI-mCherry bidirectional response plasmid
631339

Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)

pTRE3G-BI-ZsGreen1 bidirectional response plasmid
631165

Tet-On 3G Inducible Expression System (with mCherry)

pTRE3G-mCherry IRES bicistronic response plasmid
631164

Tet-On 3G Inducible Expression System (with ZsGreen1)

pTRE3G-ZsGreen1 IRES bicistronic response plasmid
Tet-On 3G Inducible Expression Systems with EF-1 Alpha Regulator Vectors
ProductRegulator vectorResponse vector
631167

Tet-On 3G Inducible Expression System (EF1alpha Version)

 

 

 

 

pEF1a-Tet3G regulator plasmid

pTRE3G response plasmid
631340

Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)

pTRE3G-BI bidirectional response plasmid
631346

Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic)

pTRE3G-IRES bicistronic response plasmid
631341

Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)

pTRE3G-BI-mCherry bidirectional response plasmid
631342

Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)

pTRE3G-BI-ZsGreen1 bidirectional response plasmid
631347

Tet-On 3G Inducible Expression System (EF1alpha, mCherry)

pTRE3G-mCherry IRES bicistronic response plasmid
631348

Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)

pTRE3G-ZsGreen1 IRES bicistronic response plasmid

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  • CRISPR screening
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  • DNA-seq
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  • Real-time PCR kits
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  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
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  • TB Green qPCR sale
  • PrimeSTAR enzyme promo
  • Try BcaBEST DNA Polymerase ver.2.0
  • RNA purification sale
  • Capturem IP and Co-IP sale
  • Baculovirus titration kits early access program
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