Tet-On 3G inducible expression plasmid systems—kit components
All plasmid-based versions of the Tet-On 3G System contain the following seven components:
- 10 μg regulator plasmid (see tables below)
- 10 μg response plasmid (see tables below)
- 10 μg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or pTRE-BI-Luc)
- 2 μg Linear Hygromycin Marker (also sold separately as Cat. # 631625)
- 2 μg Linear Puromycin Marker (also sold separately as Cat. # 631626)
- 100 rxns Xfect transfection reagent (also sold separately as Cat. # 631317)
- 50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. # 631105)
Use the tables below to pick the specific vectors and system that meet your needs. If you prefer a system that uses the human EF1-alpha promoter to express the transactivator protein, please refer to the second table below. EF1-alpha systems are recommended for use in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. Note that lentiviral, retroviral, and adenoviral Tet-On 3G inducible expression systems are also available.
| Tet-On 3G Inducible Expression Systems with CMV Regulator Vectors | |||
|---|---|---|---|
| Cat. # | Product | Regulator vector | Response vector |
| 631168 |
|
 |
|
| 631337 |  |
||
| 631166 |  |
||
| 631338 |
Tet-On 3G Bidirectional Inducible Expression System (with mCherry) |
 |
|
| 631339 |
Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1) |
 |
|
| 631165 |  |
||
| 631164 |  |
||
| Tet-On 3G Inducible Expression Systems with EF-1 Alpha Regulator Vectors | |||
|---|---|---|---|
| Product | Regulator vector | Response vector | |
| 631167 |
|
 |
|
| 631340 |
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version) |
 |
|
| 631346 |
Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic) |
 |
|
| 631341 |
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry) |
 |
|
| 631342 |
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1) |
 |
|
| 631347 |  |
||
| 631348 |  |
||
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