Tet-On 3G inducible expression plasmid systems—kit components

All plasmid-based versions of the Tet-On 3G System contain the following seven components:

• 10 μg regulator plasmid (see tables below)
• 10 μg response plasmid (see tables below)
• 10 μg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or pTRE-BI-Luc)
• 2 μg Linear Hygromycin Marker (also sold separately as Cat. # 631625)
• 2 μg Linear Puromycin Marker (also sold separately as Cat. # 631626)
• 100 rxns Xfect transfection reagent (also sold separately as Cat. # 631317)
• 50 ml Tet System Approved FBS, US Sourced (also sold separately as Cat. # 631105)

Use the tables below to pick the specific vectors and system that meet your needs. If you prefer a system that uses the human EF1-alpha promoter to express the transactivator protein, please refer to the second table below. EF1-alpha systems are recommended for use in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. Note that lentiviral, retroviral, and adenoviral Tet-On 3G inducible expression systems are also available.

Tet-On 3G Inducible Expression Systems with CMV Regulator Vectors
Cat. #	Product	Regulator vector	Response vector
631168	Tet-On 3G Inducible Expression System
631337	Tet-On 3G Bidirectional Inducible Expression System
631166	Tet-On 3G Inducible Expression System (Bicistronic Version)
631338	Tet-On 3G Bidirectional Inducible Expression System (with mCherry)
631339	Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)
631165	Tet-On 3G Inducible Expression System (with mCherry)
631164	Tet-On 3G Inducible Expression System (with ZsGreen1)

Tet-On 3G Inducible Expression Systems with EF-1 Alpha Regulator Vectors
	Product	Regulator vector	Response vector
631167	Tet-On 3G Inducible Expression System (EF1alpha Version)
631340	Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)
631346	Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic)
631341	Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)
631342	Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
631347	Tet-On 3G Inducible Expression System (EF1alpha, mCherry)
631348	Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)