Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors
AcGFP1 has 94% homology to EGFP at the amino acid level.
- AcGFP1 and EGFP have similar excitation and emission maxima.
- AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. EGFP has been reported to form dimers (Jain et al. 2001; Chen et al. 2002).
Another alternative to EGFP is ZsGreen1, a very bright, tetrameric green fluorescent protein that is ideal for reporter studies. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells (Bell et al. 2007).
| EGFP vector conversion | ||
|---|---|---|
| Old EGFP vector | Suggested replacement | Application |
| pBI-EGFP Tet Vector | Simultaneous, equivalent tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter | |
| pCMS-EGFP Vector | Express your protein of interest and AcGFP1 from the same transcript | |
| pCRE-D2EGFP Vector | Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal | |
| pD1EGFP-N1 Vector | Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover | |
| pD2EGFP Vector | Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover | |
| pD2EGFP-1 Vector | Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover | |
| pD2EGFP-N1 Vector | Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover | |
| pD4EGFP-N1 Vector | Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover | |
| pD4EGFP-Bid Vector | Monitor Bid translocation in living cells with DsRed2 | |
| pDNR-EGFP Donor Reporter Vector | Pre-linearized vectors for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1 | |
| pEGFP Vector | Express AcGFP1 in bacterial systems | |
| pEGFP-1 Vector | Monitor the activity of promoters and promoter/enhancer combinations with an AcGFP1 reporter | |
| pEGFP-Actin Vector | Visualize the actin cytoskeleton with AcGFP1 | |
| pEGFP-C1 Vector | Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning | |
| pEGFP-C2 Vector | Express and visualize a protein of interest fused to AcGFP1 | |
| pEGFP-C3 Vector | Express and visualize a protein of interest fused to AcGFP1 | |
| pEGFP-Endo Vector | Monitor intracellular membrane traffic with AcGFP1 | |
| pEGFP-F Vector | Label the inner leaflet of the plasma membrane with AcGFP1 and select with either G418 or hygromycin | |
| pEGFP-N Vector Set | Express and visualize a protein of interest fused to AcGFP1 | |
| pEGFP-N1 Vector | Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning | |
| pEGFP-N2 Vector | Express and visualize a protein of interest fused to AcGFP1 | |
| pEGFP-N3 Vector | Express and visualize a protein of interest fused to AcGFP1 | |
| pEGFP-Peroxi Vector | Label peroxisomes with DsRed2 | |
| pEGFP-Tub Vector | Visualize the microtubule network with AcGFP1 | |
| pHygEGFP Vector | Establish stable cell lines coexpressing AcGFP1 by selecting with hygromycin. Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines | |
| pIRES2-EGFP Vector | Express your protein of interest and AcGFP1 from the same transcript | |
| pIRES-EGFP Vector | Express your protein of interest and AcGFP1 from the same transcript | |
| pLEGFP-C1 Retroviral Vector | Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells | |
| pLEGFP-N1 Retroviral Vector | Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells | |
| pLP-EGFP-C1 Acceptor Vector | Pre-linearized vector for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1 | |
| pLPS-3'EGFP Acceptor Vector | Pre-linearized vectors for simple, one-step In-Fusion cloning of your gene of interest fused to AcGFP1 | |
| pNeoEGFP Vector | Establish stable cell lines expressing AcGFP1 or AcGFP1 fusion proteins by selecting with G418 | |
| pNF-KB-D2EGFP Vector | Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal | |
| pREVTRE2-d1EGFP Vector | Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1 | |
| pTRE-D2EGFP Vector | Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1 | |
| pTRE-Tight-EGFP Vector | Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1 | |
| Signaling Probe CREB-EGFP | Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal | |
References
Bell, P. et al. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies. J. Histochem. Cytochem. 55, 931–9 (2007).
Chen, Y., Müller, J. D., Ruan, Q. & Gratton, E. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys. J. 82, 133–44 (2002).
Jain, R. K., Joyce, P. B., Molinete, M., Halban, P. A. & Gorr, S. U. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Biochem. J. 360, 645–9 (2001).
Fluorescent protein related links
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