ICELL8 cx technical specifications
The ICELL8 cx Single-Cell System is an all-in-one automated, open platform for high-throughput single-cell isolation, visualization, and processing. The system combines a multisample nanodispenser (MSND) and imaging system into one unit, allowing for a smoother workflow. Cell and reagent dispensing into the 5,184-nanowell chips is automated using the system's nanodispensing technology, which can accommodate up to eight samples at a time. The imaging system is designed for scanning and acquiring images of samples (e.g., cells, nuclei, or spheroids) dispensed into an ICELL8 cx chip. The use of the system's imaging channels with cellular dyes allows for the assessment of cell viability. The included CELLSTUDIO Software provides an open architecture for experiment design and integrates instrument control, imaging, and cell selection tools.
ICELL8 cx system specifications and lab requirements
| Category | Specification |
|---|---|
| Dispense volume | 35 nl, 50 nl, or 100 nl per nanowell |
| Dispenser nozzle bore size | 125 µm |
| Software | ICELL8 cx CELLSTUDIO v2.0 Software (preinstalled) |
| Computer | Windows 10 enterprise-class PC (included with the system) |
| Power requirements (for different electrical grid types) | 120 VAC/60 Hz mains: four 120 V, 15 or 20 A circuits 220–240 VAC/50 Hz mains: four 10 A outlets 100 VAC/50–60 Hz mains: four 15 A outlets |
| Fuses | Dispenser: 4 Ah, 250 V |
| Environmental conditions | Ambient temperature: 15–30°C Relative humidity, noncondensing: 30–70% Altitude: <2,000 m from sea level Pollution degree: 2 or less |
| Dimensions | Desktop computer tower: 7" W x 14.5" H x 17.5" D (18 cm x 37 cm x 44 cm) ICELL8 cx module: 42.5" W x 23" H x 23" D (108 cm x 58 cm x 58 cm) |
| Bench space | Bench space including clearance for ICELL8 cx module and desktop computer: 70" W (minimum) x 38" H (minimum) x 30" D (178 cm x 97 cm x 76 cm) Note: Bench space must be capable of supporting 200 pounds (91 kg) |
| Floor space | Waste container: 10" W x 10" H x 10" D (25 cm x 25 cm x 25 cm) |
| Weight | 200 pounds |
| Reagents | Filtered water: Milli-Q or Elga system Reagent-grade 100% isopropyl alcohol (for priming system) 0.2% sodium hypochlorite solution (from commercial bleach solution with a date of manufacture of less than one year, diluted with filtered water) |
| Centrifuge | Access to a centrifuge such as Eppendorf 5810, Eppendorf 5810R, or equivalent |
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