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  • ‹ Back to Apollo library prep system introduction
  • Automated VeriSeq library preparation for PGS
  • Apollo library prep system citations
  • SMART-Seq v4 chemistry for the Apollo system
  • Apollo library prep system overview
  • Apollo system technical specifications
  • In-tip bead separation on the Apollo system
Apollo NGS automation solutions NGS automation solutions
Learn more about validated chemistries on the Apollo Library Prep System. Trusted chemistries for Apollo automation
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Learn more about validated chemistries on the Apollo Library Prep System. Trusted chemistries for Apollo automation
Tech Note

Automated VeriSeq library preparation for PGS

In vitro fertilization (IVF) efforts paired with preimplantation genetic screening (PGS) methods increase favorable pregnancy outcomes by identifying aneuploidy in embryos and excluding them from implantation. Used extensively for PGS, the VeriSeq™ PGS Kit (Illumina) screens all 24 chromosomes for aneuploidy in a single NGS assay. As VeriSeq library preparation requires exquisite accuracy and timeliness, our Apollo Library Prep System offers an excellent, automated solution to increase reliability, reproducibility, and efficiency when preparing these libraries and allows users to perform other tasks during the walkaway workflow.

  • Precision automation—increase reliability and efficiency by decreasing variability and human error
  • Walkaway workflow—save up to ~6 hours of hands-on time
  • High-throughput capability—generate up to 96 VeriSeq libraries in 13 hours
Precision automation for maximum recovery Streamlined, walkaway workflow High-throughput, reproducible prep Conclusions

Precision automation for maximum recovery  

The Apollo Library Prep System is a compact benchtop system with no additional accessories required, making it easy to integrate into a clinical laboratory. Using the Apollo system is easy: clear, complete setup instructions are provided at each step of the workflow, and users can simply walk away from the instrument after starting the protocol. The Apollo instrument has two Peltier blocks that are ideal for long incubations and overnight reactions, and it also offers unique in-tip magnetic bead separation for bead-purification steps (Figure 1). This in-tip separation removes the need for spinning down, eliminating the risk of bead compaction or disruption and resulting in higher sample recovery. Combined, these features provide precision automation in an enclosed environment, enabling increased sample yield along with decreased variability and human error. You can learn more on how the Apollo system increases recovery by viewing our application note, which contains a video of in-tip bead separation in action.

Automated in-tip magnetic bead separation on the Apollo Library Prep System.

Figure 1. In-tip bead separation on the Apollo Library Prep System. After the suspended bead solution is pulled up into the tip (Panel A) a retractable magnetic bar comes down to capture the beads (Panel B). The solution is automatically pipetted up and down to collect all of the beads for downstream processing.

Streamlined, walkaway workflow  

The Apollo system can save nearly six hours of hands-on time compared to manual VeriSeq library prep. This decreased hands-on time allows researchers to perform other tasks such as data analysis, experimental planning and execution, or sample prep for future runs. The automated workflow has five steps, with all steps but PCR thermal cycling performed on the Apollo system (Figure 2). Each step is easy to set up by following the user guide that clearly identifies exactly which consumable you will need, how much reagent will be used, and where consumables and reagents will be placed on the deck (Figure 3). All you need to do is set up the deck, hit [RUN], and walk away.

Workflow schematic for VeriSeq library prep on the Apollo Library Prep System

Figure 2. A simple, five-step protocol for automated VeriSeq library prep on the Apollo system. 

Example user guide schematic for preparing VeriSeq libraries on the Apollo Library Prep System

Figure 3. Example user guide schematic for VeriSeq library prep on the Apollo system. The example given is for the "Tagmentation" step and identifies consumables, reagent usage and volume, and deck placement.

High-throughput, reproducible prep  

Consistency is key when processing multiple samples for PGS. While manual VeriSeq library prep is time-consuming and subject to human error, automated VeriSeq library prep on the Apollo system is reproducible and generates up to 96 VeriSeq libraries in sets of 12, allowing easy scaling for varied sample throughput. The precision of this automated approach ensures uniform library preparation (Figure 4), which translates into more consistent sequencing metrics (Figure 5).

Uniform library preparation and normalization across multiple samples.

Figure 4. Uniform library preparation with the Apollo system. Bioanalyzer traces of nearly two dozen post-PCR VeriSeq libraries demonstrates uniform library size distribution and relative quantity.

Comparison of sample index reads between bench- and Apollo-normalized products.

Figure 5. Comparison of sample index reads between bench- and Apollo-normalized products. Sequencing results demonstrate index uniformity (e.g., % reads per index that passed filter [PF]) across 24 samples and two instruments (PS and X8) in Apollo- versus bench-normalized products. 

Conclusions  

Our Apollo Library Prep System enables an easy, walkaway workflow for generating VeriSeq libraries for PGS studies. This high-throughput system facilitates the automated generation of 96 VeriSeq libraries in 13 hours and offers increased reliability, reproducibility, and efficiency compared to bench-prepared libraries, with minimal hands-on time. Learn more about the Apollo system and its capabilities by visiting our Apollo learning center.


Apollo citations

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Our collaborators and customers are constantly making scientific breakthroughs. Take a look at their published results, obtained using the Apollo Library Prep System.

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