The Apollo Library Prep System is a compact benchtop system with no additional accessories required, making it easy to integrate into a clinical laboratory. Using the Apollo system is easy: clear, complete setup instructions are provided at each step of the workflow, and users can simply walk away from the instrument after starting the protocol. The Apollo instrument has two Peltier blocks that are ideal for long incubations and overnight reactions, and it also offers unique in-tip magnetic bead separation for bead-purification steps (Figure 1). This in-tip separation removes the need for spinning down, eliminating the risk of bead compaction or disruption and resulting in higher sample recovery. Combined, these features provide precision automation in an enclosed environment, enabling increased sample yield along with decreased variability and human error. You can learn more on how the Apollo system increases recovery by viewing our application note, which contains a video of in-tip bead separation in action.
- SmartChip Real-Time PCR System introduction
- ICELL8 introduction
- Apollo library prep system introduction
Automated VeriSeq library preparation for PGS
In vitro fertilization (IVF) efforts paired with preimplantation genetic screening (PGS) methods increase favorable pregnancy outcomes by identifying aneuploidy in embryos and excluding them from implantation. Used extensively for PGS, the VeriSeq™ PGS Kit (Illumina) screens all 24 chromosomes for aneuploidy in a single NGS assay. As VeriSeq library preparation requires exquisite accuracy and timeliness, our Apollo Library Prep System offers an excellent, automated solution to increase reliability, reproducibility, and efficiency when preparing these libraries and allows users to perform other tasks during the walkaway workflow.
- Precision automation—increase reliability and efficiency by decreasing variability and human error
- Walkaway workflow—save up to ~6 hours of hands-on time
- High-throughput capability—generate up to 96 VeriSeq libraries in 13 hours
Precision automation for maximum recovery
Streamlined, walkaway workflow
The Apollo system can save nearly six hours of hands-on time compared to manual VeriSeq library prep. This decreased hands-on time allows researchers to perform other tasks such as data analysis, experimental planning and execution, or sample prep for future runs. The automated workflow has five steps, with all steps but PCR thermal cycling performed on the Apollo system (Figure 2). Each step is easy to set up by following the user guide that clearly identifies exactly which consumable you will need, how much reagent will be used, and where consumables and reagents will be placed on the deck (Figure 3). All you need to do is set up the deck, hit [RUN], and walk away.
High-throughput, reproducible prep
Consistency is key when processing multiple samples for PGS. While manual VeriSeq library prep is time-consuming and subject to human error, automated VeriSeq library prep on the Apollo system is reproducible and generates up to 96 VeriSeq libraries in sets of 12, allowing easy scaling for varied sample throughput. The precision of this automated approach ensures uniform library preparation (Figure 4), which translates into more consistent sequencing metrics (Figure 5).
Our Apollo Library Prep System enables an easy, walkaway workflow for generating VeriSeq libraries for PGS studies. This high-throughput system facilitates the automated generation of 96 VeriSeq libraries in 13 hours and offers increased reliability, reproducibility, and efficiency compared to bench-prepared libraries, with minimal hands-on time. Learn more about the Apollo system and its capabilities by visiting our Apollo learning center.
See how scientists use the Apollo system!
Our collaborators and customers are constantly making scientific breakthroughs. Take a look at their published results, obtained using the Apollo Library Prep System.View citations
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.