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  • ‹ Back to Apollo reagents
  • Next-generation sequencing
    • RNA-seq
    • DNA-seq
    • Single-cell NGS automation
    • Bioinformatics tools
    • Whole genome amplification
    • Immune profiling
    • Epigenetics and small RNA sequencing
    • NGS accessories
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • mRNA and cDNA synthesis
  • COVID-19 research
Apollo system technology overview Apollo system overview
ThruPLEX Plasma-Seq product page ThruPLEX Plasma-Seq kit
Apollo filter tips Apollo consumables
Get answers for your questions about automated sequencing workflows. Get answers to your SMARTer Apollo questions
Home › Products › Next-generation sequencing › Apollo system › Apollo reagents › Automated NGS library prep workflows

Product Index

  • Next-generation sequencing
    • RNA-seq
      • Single-cell RNA-seq
        • SMART-Seq Single Cell for scRNA-seq
        • SMART-Seq Stranded for total RNA-seq
      • Ultra-low input RNA-seq
        • SMART-Seq v4 for mRNA-seq
        • SMART-Seq v4 3’ DE for mRNA-seq end counting
        • SMART-Seq DE3 Demultiplexer
      • Stranded RNA-seq for mammalian samples
        • Pico-input strand-specific total RNA-seq for mammalian samples v3
        • Pico-input strand-specific total RNA-seq for mammalian samples v2
        • Low-input strand-specific total RNA-seq for mammalian samples
        • High-input strand-specific total RNA-seq for mammalian samples
      • Stranded RNA-seq for nonmammalian samples
      • Kits for automation
        • SMART-Seq HT for streamlined mRNA-seq
        • SMART-Seq v4 for mRNA-seq with the Fluidigm C1
      • cDNA synthesis for Illumina or Ion Torrent
      • Targeted RNA-seq for Illumina
      • SMARTer RNA Unique Dual Index Kits
      • Legacy kits for RNA-seq
    • DNA-seq
      • PicoPLEX Gold for single cells
      • DNA-seq for FFPE and cell-free DNA
      • Low-input library prep for Illumina
      • DNA index kits
      • Legacy DNA-seq kits
    • Single-cell NGS automation
      • ICELL8 cx Single-Cell System
      • ICELL8 cx applications
        • Single-cell full-length transcriptome analysis
        • Human TCR profiling and 5' DE
        • Single-cell differential expression analysis
      • ICELL8 cx chips and consumables
      • ICELL8 applications
        • Single-cell full-length transcriptome analysis
        • Human TCR profiling and 5' DE
        • Single-cell differential expression analysis
        • Single-cell ATAC-seq
      • ICELL8 chips and consumables
    • Bioinformatics tools
      • Cogent NGS Analysis Pipeline
      • Cogent NGS Discovery Software
      • Cogent NGS Immune Profiler Software
      • ICELL8 scTCR Analyzer
      • SMARTer Human scTCR Demultiplexer
    • Whole genome amplification
      • Whole-genome amplification
    • Immune profiling
      • Human repertoire
        • Human BCR profiling kit for Illumina sequencing
        • Human TCRv2 profiling kit for Illumina sequencing
        • Human TCR profiling kit for Illumina sequencing
        • Human scTCR profiling kit for Illumina sequencing
      • Mouse repertoire
        • Mouse BCR profiling kit for Illumina sequencing
        • Mouse TCR profiling kit for Illumina sequencing
    • Epigenetics and small RNA sequencing
      • ChIP-seq library construction
      • Meth-seq kit
      • Small RNA-seq kit
    • NGS accessories
      • Ribosomal RNA removal
      • SeqAmp DNA Polymerase
      • Library Quantification Kit
      • Magnetic separator
      • Single-cell lysis buffer
  • Gene function
    • Gene editing
      • CRISPR-Cas9
        • Long ssDNA for knockins
        • Knockin screening kit
        • Genome-wide sgRNA library system
        • Recombinant Cas9 protein
        • GMP recombinant Cas9
        • Mutation detection kits
        • In vitro transcription and screening kits
        • Cas9-sgRNA gesicle production
        • Cas9 antibodies
        • Plasmid systems
        • Lentiviral systems
        • AAV systems
        • RNA transfection
        • Genotype confirmation kit
        • Indel identification kit
      • Cre recombinase
        • AAV2-Cre recombinase
        • Cre Recombinase Gesicles
        • Cre recombinase RLPs
    • Viral transduction
      • Adeno-associated virus (AAV)
        • Vector systems
          • Helper-free expression system (CMV promoter)
          • Tet-inducible promoter
          • CRISPR/Cas9 system
          • Cre recombinase system
          • Beta-galactosidase system
          • ZsGreen1 control vector
        • qPCR titration
        • Purification kits
        • AAV concentration
        • Packaging systems and cells
          • 293T cell line
          • Extraction solutions
          • Packaging plasmid sets
      • Adenovirus
        • Vectors and packaging
          • Expression system 3
          • Tet inducible
          • Adeno-X 293 cells
        • CAR Receptor Booster
        • Purification kits
        • Titration kits
      • Lentivirus
        • Vector systems
          • Constitutive promoter
          • EF-1 alpha promoter
          • IRES bicistronic
          • Fluorescent protein
          • Tet-inducible
        • Premade lentiviral particles
          • Whole-cell labeling
          • Organelle labeling
          • Tet-On 3G transactivator
        • Packaging systems and cells
          • Lenti-X packaging single shots
          • Lenti-X 293T cells
        • Titration kits
          • Lenti-X GoStix Plus
          • qRT-PCR
          • p24 ELISA
          • Integrated copy number
        • Lentivirus concentration
        • Purification kits
        • Integration site analysis
        • Transduction enhancers
          • Lenti-X Accelerator
          • Ecotropic Receptor Booster
      • Retrovirus
        • Vector systems
          • Constitutive promoter
          • Fluorescent protein
          • Tet inducible
          • MSCV system
        • Packaging systems and cells
        • Titration kits
        • Retro-X Concentrator
        • Integration site analysis
        • Receptor booster
    • Fluorescent proteins
      • Fluorescent protein plasmids
        • Cyan and green fluorescent proteins
          • AcGFP1 fluorescent protein
          • ZsGreen1 fluorescent protein
          • GFP & GFPuv fluorescent proteins
          • AmCyan1 fluorescent protein
        • Red fluorescent proteins
          • mCherry fluorescent protein
          • DsRed-Monomer fluorescent protein
          • DsRed2 fluorescent protein
          • DsRed-Express and DsRed-Express2 fluorescent proteins
          • tdTomato fluorescent protein
          • AsRed2 fluorescent protein
          • mStrawberry fluorescent protein
        • Far-red fluorescent proteins
          • E2-Crimson fluorescent protein
          • HcRed1 fluorescent protein
          • mRaspberry fluorescent protein
          • mPlum fluorescent protein
        • Orange and yellow fluorescent proteins
          • mOrange2 fluorescent protein
          • mBanana fluorescent protein
          • ZsYellow1 fluorescent protein
        • Photoactivatable and photoswitchable proteins
          • Dendra2 fluorescent protein
          • Timer fluorescent protein
          • PAmCherry fluorescent protein
      • Subcellular labeling plasmids
      • Flow cytometer calibration beads
      • Recombinant fluorescent proteins
    • T-cell transduction and culture
      • RetroNectin reagent
      • LymphoONE T-cell medium
      • Cytokine GoStix Plus assays
      • Anti-CD3 antibody (OKT3)
      • CultiLife culture bags
      • RetroNectin ELISA kit
    • Tet-inducible expression systems
      • Tet-One systems
      • Tet-On 3G systems
        • Tet-On 3G systems
        • Tet-On 3G—lentiviral
        • Tet-On 3G—retroviral
        • Adeno-X Tet-On 3G inducible expression system
        • Tet-On 3G cell lines
        • Tet-On 3G systems—bidirectional
        • Tet-On 3G systems—bicistronic
        • Tet-On 3G systems—EF1-alpha promoter
      • Tet systems legacy products
        • Tet-Off cell lines
        • Tet-On and Tet-Off—2nd generation
        • Tet-tTS transcriptional silencer
      • Tet-inducible miRNA systems
      • TetR monoclonal antibody
      • Tet-approved FBS
    • ProteoTuner protein control systems
      • Plasmid systems
      • Viral systems
      • ProteoTuner antibody
      • Shield1 ligand
    • iDimerize inducible protein interaction systems
      • Homodimerization systems
      • Heterodimerization systems
      • Reverse dimerization
      • Regulated transcription
      • Dimerizer ligands
      • In vivo applications
      • Dimerization domain antibodies
    • Transfection reagents
      • Plasmid transfection reagents
        • Xfect reagent
        • Xfect for mES cells
        • Calcium phosphate transfection
        • Xfect single shots
        • Fluorescent transfection controls
      • Protein transfection reagents
      • RNA transfection reagents
    • Mammalian expression plasmids
      • Plasmids with selectable markers
      • Bidirectional promoter vectors
      • Bicistronic IRES vectors
      • MicroRNA expression
    • Cell biology assays
      • Extracellular vesicle isolation
      • Reporter systems
        • Fluorescent protein promoter reporters
          • Promoterless (lowest background)
          • Promoterless (traditional)
        • Proteasome monitoring
        • Secreted alkaline phosphatase assays
        • Beta galactosidase and LacZ vectors
        • Secreted luciferase assay
        • Fucci cell-cycle vectors
        • Lenti-X Actin Dynamics Monitoring Kit
      • Apoptosis detection kits
        • Annexin V apoptosis assay
        • PARP antibody
        • ApoAlert caspase assays
        • Apoptosis analysis
        • In situ apoptosis detection
      • Epigenetics
        • Detecting DNA and chromatin modifications
          • Methylation-dependent restriction enzyme
        • DNA methylation
          • EpiXplore Methylated DNA Enrichment Kit
          • EpiScope MSP Kit
          • EpiScope Nucleosome Preparation Kit
          • EpiTaq HS
          • EpiScope control DNA
      • Cell biology reagents
        • WST-1 cell proliferation
        • Heme oxygenase-1
      • RNA interference
        • miRNA
          • MicroRNA quantitation
        • siRNA
          • siRNA quantitation
      • Cell-culture accessories
        • Magnetic separator for cell culture
        • Antibiotics for cell biology
          • Anhydrotetracycline
        • Antibiotic selection markers & plasmids
      • Signal transduction
        • TransFactor kits
        • Pathway profiling vectors
        • Dominant negative vectors
  • Stem cell research
    • Media and supplements
      • Pluripotent stem cell media
        • Cellartis DEF-CS 500 Culture System
        • Xeno-free human stem cell culture medium
        • Xeno-free GMP-grade stem cell culture medium
        • Cellartis 2i mES/iPSC Culture Medium
        • Cellartis 3i mES/iPSC Culture Medium
        • Xeno-free human MSC culture medium
      • Stem cell differentiation media and kits
        • iPS cell to hepatocyte differentiation system
        • Hepatocyte differentiation kit
        • Definitive endoderm differentiation kit
        • RHB-A
        • NDiff 227
        • Osteoblast-inducer reagent
      • Stem cell matrices
        • iMatrix-511
      • Primary hepatocyte medium
    • Stem cells and stem cell-derived cells
      • Human stem cell-derived intestinal epithelial cells
      • Human embryonic stem cells
      • Human induced pluripotent stem cells
      • Human stem cell-derived definitive endoderm cells
      • Human stem cell-derived beta cells
      • Human stem cell-derived hepatocytes
      • Human stem cell-derived cardiomyocytes
    • Single-cell cloning of edited hiPS cells
    • Accessories
      • Pluripotency check PCR kit
      • Mouse feeder cell quantification kit
      • Human mitochondrial DNA monitoring
  • Protein research
    • Purification products
      • Antibody purification
        • Capturem Protein A
        • Capturem Protein G
        • Thiophilic resin
      • His-tagged protein purification
        • Capturem high-capacity membranes
        • Bulk resins and gravity columns
          • Cobalt resin
          • Cobalt columns—gravity
          • Cobalt columns—spin
          • Cobalt resin—cell lysate
          • Nickel resin
          • Nickel columns—gravity
        • Resin in FPLC cartridges
          • Cobalt resin—FPLC
          • Cobalt columns—FPLC
          • Nickel columns—FPLC
        • Magnetic beads
          • Cobalt resin—magnetic
          • Nickel resin—magnetic
      • Capturem Streptavidin
      • Phosphoprotein purification
        • Phosphopeptide spin columns
        • Phosphoprotein magnetic kit
        • Phosphoprotein enrichment kit
      • GST-tagged protein purification
      • Protease inhibitors
        • ProteoGuard
        • Calpastatin
      • xTractor cell lysis buffers
    • Two-hybrid and one-hybrid systems
      • Yeast two-hybrid system
        • Matchmaker Gold Yeast Two-Hybrid System
        • Yeast two-hybrid libraries
          • Normalized Mate & Plate Libraries
          • Mate & Plate libraries
        • Yeast two-hybrid library construction
        • Yeast two-hybrid vectors
        • Yeast strains
        • Prey rescue by colony PCR
      • Yeast one-hybrid system
      • Mammalian two-hybrid system
      • Yeast media
        • Aureobasidin A
        • Yeast two-hybrid media
        • Yeast one-hybrid media
        • Minimal media pouches (ready-mixed)
        • YPDA and YPD rich media
        • SD base
        • Amino acid dropout mixes
        • X-alpha-Gal
      • Yeast transformation kits
        • Yeastmaker transformation system
        • Quick & Easy Yeast Transformation Mix
      • Yeast plasmid-isolation minipreps
    • Mass spectrometry reagents
      • Capturem trypsin digestion for mass spectrometry
      • Capturem pepsin digestion
      • Trypsin digestion—TPCK magnetic trypsin
    • Expression vectors & systems
      • Mammalian expression
        • pHEK293 Ultra Expression Vectors
        • pCMV-Myc & pCMV-HA Vector Set
        • pCMV-DYKDDDDK Vector Set (FLAG tag)
      • E. coli expression systems
        • pET expression system
        • HAT-tag expression system
        • MazF single-protein production
        • mRNA interferase-MazF enzyme
      • Baculovirus expression system
        • Baculovirus expression system
        • Baculovirus titration kits
        • Insect cells
      • Brevibacillus expression system
        • Brevibacillus expression systems
        • Control vector for Brevibacillus expression
      • B. subtilis expression system
      • Human Cell-Free Protein Expression System
      • Protein folding kits
        • Chaperone plasmid set
        • pCold DNA cold shock
        • pCold ProS2 DNA
        • pCold TF DNA
    • Glycobiology
      • Alpha 1,2-L Fucosidase
      • Alpha 1, 3/4-L Fucosidase
      • Cloned alpha 2,3-Sialidase
      • Glycopeptidase F
      • rEGCase
      • Lacto-N-biosidase
      • SCDase
      • Sialic acid kit
      • Glycoprotein enrichment and detection
    • Antibodies and immunoprecipitation
      • Capturem IP & Co-IP Kit
      • Myc, HA, & 6xHis tag antibodies
      • Myc-tagged & HA-tagged vectors
      • c-Myc agarose beads
      • Magnetic Myc IP Kit
      • Magnetic Beads Immunoprecipitation Buffer Set
      • GAL4 antibodies
    • SDS-PAGE & western blotting
      • Buffers and powders
        • EDTA Powder
        • Phosphate buffered salts (PBS)
        • SDS powder
        • SSC powder
        • Tris powder
        • Tris-EDTA Powder
        • Tris-acetate-EDTA (TAE) powder
        • Tris-Borate-EDTA powder
        • Tris-buffered saline (TBS) powder
        • Tris-glycine powder
        • Tris-glycine-SDS powder
      • Western blotting reagents
        • Western BLoT HRP detection
        • Western BLoT Immuno Booster
        • Western BLoT Stripping Buffer
        • Western BLoT Blocking Buffer
        • Protein gel staining
        • Western BLoT Rapid Detect v2.0
      • Protein molecular weight markers
        • Low-, high-, and broad-range
        • CLEARLY Protein Ladder sets
      • Tissue-specific protein extracts
    • Protein sequencing
      • Protein fragmentation
        • Arginylendopeptidase
        • Pfu Protease S
      • N-terminal deblocking and analysis
        • Pfu Aminopeptidase I
        • Pfu Methionine Aminopeptidase
        • Pfu N-acetyl Deblocking Aminopeptidase (Ac-DAP)
        • Pfu Pyroglutamate Aminopeptidase
    • Accessory enzymes
      • Corystein (Purothionin) reagent
      • HRV 3C Protease
      • Westase
  • PCR
    • Most popular polymerases
    • Standard PCR
      • Takara Taq products
        • Takara Taq and premix
        • Takara Taq PCR kit
      • Carryover-prevention products
        • Uracil DNA Glycosylase (UNG), Heat-Labile
    • High-yield PCR
      • Titanium Taq products
        • Titanium Taq DNA Polymerase
        • Titanium Taq PCR kit
        • Titanium Taq kit for SNP arrays
      • Advantage 2 products
        • Advantage 2 Polymerase Mix
        • Advantage 2 PCR Kit
        • Advantage HF 2 PCR kit
      • TaKaRa Ex Premier DNA Polymerase
      • Ex Taq products
        • Ex Taq polymerase
        • Ex Taq polymerase hot start
    • High-fidelity PCR
      • PrimeSTAR HS DNA Polymerase
      • PrimeSTAR Max DNA Polymerase
      • Advantage HD DNA polymerase
    • Fast PCR
      • SpeedSTAR HS DNA Polymerase
      • Z-Taq DNA polymerase
    • Long-range PCR
      • LA Taq products
        • LA Taq DNA polymerase
        • LA Taq DNA polymerase HS
        • LA Taq PCR kit
        • LA Taq premix
      • Long PCR controls
    • GC rich PCR
      • PrimeSTAR GXL DNA Polymerase
      • PrimeSTAR HS with GC buffer
      • LA Taq DNA polymerase with GC buffers
      • Advantage GC 2 polymerase
      • Advantage GC Genomic LA Polymerase Mix
    • Direct PCR
      • Terra PCR Direct mixes and kits
    • PCR master mixes
      • Multiplex
        • SuperPlex Premix
      • Lyophilized
        • Titanium Taq EcoDry premix
        • Advantage 2 EcoDry premix
      • Complex templates
        • PrimeSTAR GXL Premix Fast, Dye plus
        • PrimeSTAR GXL Premix
      • High fidelity
        • CloneAmp HiFi PCR Premix
      • Dye-added
        • EmeraldAmp GT PCR Master Mix
        • EmeraldAmp MAX products
          • EmeraldAmp MAX premix
          • EmeraldAmp MAX HS premix
        • SapphireAmp fast premix
      • Diverse samples
        • Ex Taq premix
      • Routine
        • Takara Taq HS
    • Custom business friendly and automation-ready solutions
      • Titanium Taq SP products
    • Molecular diagnostic products
      • Titanium Taq SP
    • Application-specific PCR
      • Human pathogen detection
        • Papillomavirus detection
        • Papillomavirus typing
    • Other PCR-related products
      • dNTPs
        • Advantage UltraPure dNTPs
        • dNTPs
      • Taq antibodies
        • Taq Antibody
        • TaqStart Antibody
      • DNA-OFF and RNase-OFF
      • DNA labeling kits
        • Random primer labeling
        • BcaBEST for random-primer labeling
      • QuickClean enzyme removal
  • Cloning
    • In-Fusion seamless cloning
      • In-Fusion Snap Assembly
      • In-Fusion Snap Assembly bundles
      • In-Fusion Snap Assembly EcoDry (lyophilized formula)
      • In-Fusion Snap Assembly for high-throughput workflows
      • In-Fusion HD Cloning bundles
      • Cloning Enhancer
    • Competent cells
      • Stellar Chemically Competent Cells
      • Stellar Electrocompetent Cells
      • Agrobacterium electrocompetent cells
    • Ligation kits
      • DNA ligation kits, quick
      • DNA ligation kit, long fragments
      • DNA Blunting Kit
      • LA PCR in vitro Cloning Kit
      • Mighty Cloning Reagent Set (Blunt End)
      • DNA Ligation Kit, Mighty Mix
      • Cassettes and cassette primers
      • Deletion Kit for Kilo-Sequencing
    • Mutagenesis kits
      • Site-directed mutagenesis
      • Random mutagenesis kit
    • Ligation enzymes
      • E. coli DNA Ligase
      • T4 DNA Ligase
      • T4 RNA Ligase
    • Restriction enzymes
      • AatII
      • AccI
      • AccII (FnuDII)
      • AccIII (BspMII)
      • AfaI (RsaI)
      • AflII
      • AluI
      • Aor13HI (BspMII, AccIII)
      • Aor51HI (Eco47III)
      • ApaI
      • ApaLI
      • BalI
      • BamHI
      • BanII (HgiJII)
      • BciT130I (EcoRII, MvaI)
      • BcnI (CauII)
      • BglI
      • BglII
      • BlnI (AvrII)
      • BmeT110I (AvaI)
      • BmgT120I (Cfr13I, AsuI)
      • Bpu1102I (EspI)
      • Bsp1286I (SduI)
      • Bsp1407I
      • BspT104I (AsuII, NspV)
      • BspT107I (HgiCI)
      • BssHII (BsePI)
      • BstPI (BstEII, EcoO65I)
      • BstXI
      • Cfr10I
      • ClaI
      • CpoI (RsrII)
      • DdeI
      • DraI (AhaIII)
      • DpnI
      • EaeI (CfrI)
      • Eco52I (XmaIII)
      • Eco81I (SauI)
      • EcoO109I (DraII)
      • EcoO65I (BstEII, BstPI)
      • EcoRI
      • EcoRV
      • EcoT14I (StyI)
      • EcoT22I (AvaIII)
      • FbaI (BclI)
      • FokI
      • HaeII
      • HaeIII
      • HapII (HpaII, MspI)
      • HhaI
      • HincII
      • HindIII
      • HinfI
      • HpaI
      • KpnI
      • MboI (Sau3AI)
      • MboII
      • McrBC
      • MflI
      • MluI
      • MseI (RspRSII)
      • MspI (HpaII, HapII)
      • MunI (MfeI)
      • NaeI
      • NcoI
      • NdeI
      • NheI
      • NotI
      • NruI
      • PmaCI
      • PshAI
      • PshBI (VspI)
      • Psp1406I (AclI)
      • PstI
      • PvuI
      • PvuII
      • SacI
      • SacII
      • SalI
      • Sau3AI (MboI)
      • ScaI
      • SfiI
      • SmaI
      • SmiI (SwaI)
      • SnaBI
      • SpeI
      • SphI
      • Sse8387I
      • SspI
      • StuI
      • TaqI (TthHB8I)
      • Van91I (PflMI)
      • VpaK11BI (AvaII)
      • XhoI
      • XbaI
      • XspI (BfaI, MaeI)
    • Modifying enzymes
      • DNA polymerases
        • DNA Polymerase I (Pol I)
        • DNA Polymerase I, Klenow Fragment
        • Poly(A) Polymerase
        • T4 DNA Polymerase
        • Terminal Deoxynucleotidyl Transferase
      • Nucleases
        • BAL 31 Nuclease
        • Yatalase
        • Cryonase Cold-Active Nuclease
        • Exonuclease I
        • Exonuclease III
        • Micrococcal Nuclease
        • Mung Bean Nuclease
        • Recombinant DNase I (RNase-free)
        • Ribonuclease H (RNase H)
        • S1 Nuclease
      • Phosphatases and kinases
        • Pyrophosphatase
        • Alkaline phosphatase (calf intestinal)
        • Alkaline phosphatase (E. coli)
        • Alkaline phosphatase (shrimp)
    • X-Gal and IPTG
      • X-Gal
      • IPTG (dioxane free)
    • Linkers, primers, and cloning vectors
      • Linkers and primers
        • Random primers
      • Cloning vectors
        • E. coli-yeast shuttle vectors
        • Chloramphenicol-resistant pUC plasmids
        • Kanamycin resistant pUC vectors
        • pUC118/119 vectors
        • pUC18 and pUC19 vectors
        • DNA cloning vectors
        • T-Vectors pMD20 and pMD19
        • pPTR shuttle vectors
        • M13mp18 virion DNA
        • M13mp18 RF phage vector
        • PhiX174 RF I DNA
        • pBR322 DNA vector
        • Thermus thermophilus HB8 genomic DNA
        • Lambda DNA
        • Plant transformation vectors
          • pRI 101 DNA vectors
          • Plant transformation vectors
          • pRI 909/910 DNA
    • Agarose gel electrophoresis
      • Agarose gel powders
        • Agarose L03
        • PrimeGel agarose
      • DNA and RNA markers
        • DNA molecular weight markers
        • ssRNA molecular weight marker
      • Electrophoresis instruments
        • Mupid One system
        • Mupid-2plus system
        • Mupid accessories
        • Mupid-exU system
    • Nucleic acid extraction
      • Extraction reagents
        • MightyPrep reagent for DNA extraction
        • DEXPAT reagents
        • Fruit-mate for RNA Purification
        • DNA extraction from yeast
        • RNA extraction from yeast
        • High-Salt Solution for Precipitation (Plant)
      • Proteinase K
      • BioMasher microhomogenizer
  • Nucleic acid purification
    • Plasmid purification kits
      • Miniprep
        • NucleoSpin Plasmid
        • NucleoSpin Plasmid Transfection-grade
        • NucleoSpin Plasmid EasyPure
        • NucleoBond PC 20
      • Midiprep
        • NucleoSnap Plasmid Midi
        • NucleoBond Xtra
        • NucleoBond PC 100
      • Maxiprep
        • NucleoBond Xtra
        • Nucleobond PC 500
      • Megaprep and gigaprep
        • NucleoBond PC 2000
        • NucleoBond PC 10000
      • High-throughput preps
        • NucleoSpin 8 Plasmid
        • NucleoSpin 96 Plasmid
        • NucleoSpin 96 Plasmid Transfection-grade
        • NucleoBond 96 Xtra EF
      • Endotoxin-free preps
        • NucleoBond Xtra Midi EF
        • NucleoBond Xtra Maxi EF
        • NucleoBond PC 500 EF
        • NucleoBond PC 2000 EF
        • NucleoBond PC 10000 EF
        • NucleoBond PC Prep100
      • BAC
        • NucleoBond Xtra BAC
        • NucleoBond BAC 100
        • NucleoSpin 96 Flash
    • Genomic DNA purification kits
      • Tissue
        • NucleoSpin Tissue
        • NucleoSpin 8/96 Tissue
        • NucleoMag Tissue
        • NucleoMag DNA Swab
        • NucleoSpin DNA RapidLyse
        • NucleoSpin 96 DNA RapidLyse
        • NucleoBond HMW DNA
        • NucleoBond AXG
        • NucleoSpin DNA Lipid Tissue
      • FFPE
        • NucleoSpin DNA FFPE XS
        • NucleoSpin 96 DNA FFPE
        • NucleoMag DNA FFPE
      • Blood and plasma
        • NucleoBond CB
        • NucleoSpin Blood
        • NucleoSpin 8/96 Blood
        • NucleoMag Blood
      • Forensic
        • NucleoSpin DNA Trace
        • NucleoSpin 8/96 Trace
        • NucleoMag Trace
        • NucleoMag DNA Forensic
        • NucleoSpin Forensic Filters
        • NucleoSpin DNA Forensic
      • Plant
        • NucleoSpin Plant II
        • NucleoSpin 8/96 Plant II
        • NucleoMag Plant
        • NucleoMag 384 Plant
      • Other organisms and samples
        • NucleoSpin DNA Insect
        • NucleoSpin Microbial DNA
        • NucleoMag DNA Bacteria
        • NucleoSpin DNA Yeast
      • Food
        • NucleoSpin Food
        • NucleoSpin 8/96 Food
        • NucleoMag DNA Food
    • DNA cleanup kits
      • NucleoSpin Gel and PCR Clean-Up
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Get answers for your questions about automated sequencing workflows. Get answers to your SMARTer Apollo questions

Trusted chemistries for Apollo automation

Automated NGS library prep on the Apollo system

The Apollo Library Prep System combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. You can also perform your TruSeq® stranded mRNA library prep, or generate VeriSeq™ PGS libraries, on the Apollo system to maximize your time and minimize sample loss.

The Apollo Library Prep System combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. You can also perform your TruSeq® stranded mRNA library prep, or generate VeriSeq™ PGS libraries, on the Apollo system to maximize your time and minimize sample loss. Preprogrammed scripts, including those validated for running the SMART-Seq v4 reagent kit, a ThruPLEX kit, Illumina TruSeq Stranded mRNA Library Prep kit, or VeriSeq PGS on the system, contribute to significantly reduced hands-on time compared to manual library prep methods. Simply set up, hit [RUN], and walk away to perform other tasks.

SMART-Seq v4 Reagent Kit for the Apollo System

This kit is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA using a high-throughput workflow that enables processing of up to 96 samples in a single working day. With this kit, we have reformatted our best-in-class method for ultra-low-input RNA-seq library prep for automation on the Apollo platform, providing a walkway solution that performs comparably to manual workflows. cDNA produced with this kit can be processed into NGS libraries suitable for analysis on Illumina or Ion Torrent platforms. The kit incorporates the same technologies and chemistries associated with our other SMART-Seq v4 products, including oligo(dT)-priming, SMART template-switching technology, and locked nucleic acids (LNA), ensuring the production of cDNA libraries that provide full-length sequence information, accurate representation of GC-rich transcripts, and industry-leading sensitivity.

ThruPLEX DNA Library Prep on the Apollo System

We adapted our trusted ThruPLEX chemistry for automation on the Apollo system, enabling you to start with 50 pg–30 ng of fragmented double-stranded DNA or 1–30 ng of cfDNA and efficiently process up to 48 samples per run, without wasting reagents. This versatile kit, combined with the convenience and consistency of the Apollo system, provides added sensitivity to better understand genetic variations within fragmented DNA samples from biofluids such as cell-free DNA, genomic DNA, DNA from FFPE materials, cDNA, or ChIP DNA. Once purified and quantified, the resulting library is ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System

Never perform Illumina TruSeq stranded mRNA library prep by hand again. Our automated protocol (download protocol) adapts the TruSeq Stranded mRNA library prep protocol for the Apollo system, allowing you to start with 0.1–1 µg of total RNA or 10–100 ng of previously isolated mRNA (from species with polyA tails) and process up to 48 samples per run, in multiples of six (Illumina Cat. # 20020594 for low-throughput or Cat. # 20020595 for high-throughput workflows). Once purified and quantified, the resulting library is ready for sequencing on Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated VeriSeq Library Prep for PGS on the Apollo System

Screen all 24 chromosomes for aneuploidy with the Illumina VeriSeq PGS Kit (Illumina Cat. # RH-101-1001) using our automated protocol (download protocol). This workflow leverages the Apollo system's precision automation to allow the generation of up to 96 VeriSeq libraries in 13 hours, increasing reliability and efficiency by decreasing variability and human error. Following library generation and normalization, the resulting libraries are ready for Illumina NGS using standard Illumina sequencing reagents and protocols.

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Cat. # Product Size License Quantity Details
640170 SMART-Seq® v4 Reagent Kit for the Apollo™ System 96 Rxns USD $5964.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.
*

The SMART-Seq v4 Reagent Kit for the SMARTer Apollo System is designed to generate high-quality cDNA from 10 pg–10 ng of total RNA or directly from 1–1,000 cells. This kit is specifically formulated for the SMARTer Apollo system, enabling processing of up to 96 samples in a single working day with minimal hands-on time. cDNA produced with the kit can be used to construct RNA-seq libraries that are compatible with Illumina® or Ion Torrent platforms, and that provide industry-leading sensitivity and accurate representation of rare and GC-rich transcripts.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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640170: SMART-Seq v4 Reagent Kit for the SMARTer Apollo System

640170: SMART-Seq v4 Reagent Kit for the SMARTer Apollo System

Overview

SMART-Seq v4 Reagent Kit for the Apollo System

  • Industry-leading performance—obtain accurate representation of rare and GC-rich transcripts
  • Flexible inputs—generate high-quality cDNA libraries from 1–1,000 cells or 10 pg–10 ng of total RNA
  • Walkaway automation—minimize hands-on time and sample-handling errors using Apollo scripts developed specifically for this protocol
  • High-throughput sample preparation—obtain purified cDNA from up to 96 samples in a single working day

ThruPLEX DNA Library Prep on the Apollo System

  • Unique, ligation-free method—ThruPLEX technology uses stem-loop adapters to construct high-quality libraries in a fast and efficient workflow
  • Flexible inputs—generate Illumina-ready libraries from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
  • Walkaway automation—minimize contamination and hands-on time using Apollo scripts developed specifically for this protocol

Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System (download protocol)

  • Robust RNA-seq solution—enable whole-transcriptome analysis with strand information from a wide variety of samples
  • Flexible inputs—generate Illumina-ready libraries from 0.1–1 µg of total RNA or 10–100 ng of previously isolated mRNA (from species with polyA tails)
  • Walkaway automation—minimize hands-on time and increase consistency using Apollo scripts developed specifically for running TruSeq Stranded mRNA library prep kits

Automated VeriSeq Library Prep for PGS on the Apollo System (download protocol)

  • Precision automation for PGS—increase reliability, reproducibility, and efficiency of VeriSeq library prep for PGS
  • High-throughput capability—generate up to 96 VeriSeq libraries in 13 hours
  • Walkaway workflow—save up to ~6 hours of hands-on time

More Information

Applications

  • SMART-Seq v4 Reagent Kit for the Apollo System
    • High-throughput cDNA preparation from low-input samples consisting of 1–1,000 cells or 10 pg–10 ng of total RNA
    • mRNA-seq on Illumina or Ion Torrent platforms
  • ThruPLEX DNA Library Prep on the Apollo System
    • High-throughput, Illumina-ready NGS library preparation from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
    • DNA-seq and Plasma-seq on Illumina platforms
  • Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System
    • Low- to high-throughput, Illumina-ready NGS library preparation with TruSeq technology using a validated Apollo script set and protocol
    • Whole-transcriptome sequencing on Illumina platforms
  • Automated VeriSeq Library Prep for PGS on the Apollo System
    • Low- to high-throughput preparation of Illumina-ready VeriSeq libraries using a validated Apollo script set and protocol
    • Screen all 24 chromosomes for aneuploidy in a single NGS assay on Illumina platforms

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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