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Trusted chemistries for Apollo automation

Automated NGS library prep on the Apollo system

The Apollo Library Prep System combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. You can also perform your TruSeq® stranded mRNA library prep, or generate VeriSeq™ PGS libraries, on the Apollo system to maximize your time and minimize sample loss.

The Apollo Library Prep System combines automated precision liquid handling with gold-standard chemistries, such as SMART-Seq v4 and ThruPLEX, to deliver the reliability, flexibility, and reproducible results you expect. You can also perform your TruSeq® stranded mRNA library prep, or generate VeriSeq™ PGS libraries, on the Apollo system to maximize your time and minimize sample loss. Preprogrammed scripts, including those validated for running the SMART-Seq v4 reagent kit, a ThruPLEX kit, Illumina TruSeq Stranded mRNA Library Prep kit, or VeriSeq PGS on the system, contribute to significantly reduced hands-on time compared to manual library prep methods. Simply set up, hit [RUN], and walk away to perform other tasks.

SMART-Seq v4 Reagent Kit for the Apollo System

This kit is designed to generate high-quality, full-length cDNA directly from 1–1,000 cells or 10 pg–10 ng of total RNA using a high-throughput workflow that enables processing of up to 96 samples in a single working day. With this kit, we have reformatted our best-in-class method for ultra-low-input RNA-seq library prep for automation on the Apollo platform, providing a walkway solution that performs comparably to manual workflows. cDNA produced with this kit can be processed into NGS libraries suitable for analysis on Illumina or Ion Torrent platforms. The kit incorporates the same technologies and chemistries associated with our other SMART-Seq v4 products, including oligo(dT)-priming, SMART template-switching technology, and locked nucleic acids (LNA), ensuring the production of cDNA libraries that provide full-length sequence information, accurate representation of GC-rich transcripts, and industry-leading sensitivity.

ThruPLEX DNA Library Prep on the Apollo System

We adapted our trusted ThruPLEX chemistry for automation on the Apollo system, enabling you to start with 50 pg–30 ng of fragmented double-stranded DNA or 1–30 ng of cfDNA and efficiently process up to 48 samples per run, without wasting reagents. This versatile kit, combined with the convenience and consistency of the Apollo system, provides added sensitivity to better understand genetic variations within fragmented DNA samples from biofluids such as cell-free DNA, genomic DNA, DNA from FFPE materials, cDNA, or ChIP DNA. Once purified and quantified, the resulting library is ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System

Never perform Illumina TruSeq stranded mRNA library prep by hand again. Our automated protocol (download protocol) adapts the TruSeq Stranded mRNA library prep protocol for the Apollo system, allowing you to start with 0.1–1 µg of total RNA or 10–100 ng of previously isolated mRNA (from species with polyA tails) and process up to 48 samples per run, in multiples of six (Illumina Cat. # 20020594 for low-throughput or Cat. # 20020595 for high-throughput workflows). Once purified and quantified, the resulting library is ready for sequencing on Illumina NGS instruments using standard Illumina sequencing reagents and protocols.

Automated VeriSeq Library Prep for PGS on the Apollo System

Screen all 24 chromosomes for aneuploidy with the Illumina VeriSeq PGS Kit (Illumina Cat. # RH-101-1001) using our automated protocol (download protocol). This workflow leverages the Apollo system's precision automation to allow the generation of up to 96 VeriSeq libraries in 13 hours, increasing reliability and efficiency by decreasing variability and human error. Following library generation and normalization, the resulting libraries are ready for Illumina NGS using standard Illumina sequencing reagents and protocols.

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Cat. # Product Size License Quantity Details
640170 SMART-Seq® v4 Reagent Kit for the Apollo™ System 96 Rxns USD $5964.00

The SMART-Seq v4 Reagent Kit for the SMARTer Apollo System is designed to generate high-quality cDNA from 10 pg–10 ng of total RNA or directly from 1–1,000 cells. This kit is specifically formulated for the SMARTer Apollo system, enabling processing of up to 96 samples in a single working day with minimal hands-on time. cDNA produced with the kit can be used to construct RNA-seq libraries that are compatible with Illumina® or Ion Torrent platforms, and that provide industry-leading sensitivity and accurate representation of rare and GC-rich transcripts.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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R400492 ThruPLEX® Plasma-seq 96D Kit 96 Rxns USD $3966.00

The ThruPLEX Plasma-Seq Kit builds on the innovative ThruPLEX chemistry to generate and multiplex high-complexity DNA libraries from cell-free DNA isolated from plasma. Only 1 ng to 30 ng of cell-free DNA is required for library preparation. Once purified and quantified, the resulting library is ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The entire three-step workflow takes place in a single tube or well in about two hours. No intermediate purification steps or sample transfers are necessary, preventing handling errors and loss of valuable samples. This product contains reagents for 96 reactions with 96 pre-plated dual-index primer sets.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components

Overview

SMART-Seq v4 Reagent Kit for the Apollo System

  • Industry-leading performance—obtain accurate representation of rare and GC-rich transcripts
  • Flexible inputs—generate high-quality cDNA libraries from 1–1,000 cells or 10 pg–10 ng of total RNA
  • Walkaway automation—minimize hands-on time and sample-handling errors using Apollo scripts developed specifically for this protocol
  • High-throughput sample preparation—obtain purified cDNA from up to 96 samples in a single working day

ThruPLEX DNA Library Prep on the Apollo System

  • Unique, ligation-free method—ThruPLEX technology uses stem-loop adapters to construct high-quality libraries in a fast and efficient workflow
  • Flexible inputs—generate Illumina-ready libraries from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
  • Walkaway automation—minimize contamination and hands-on time using Apollo scripts developed specifically for this protocol

Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System (download protocol)

  • Robust RNA-seq solution—enable whole-transcriptome analysis with strand information from a wide variety of samples
  • Flexible inputs—generate Illumina-ready libraries from 0.1–1 µg of total RNA or 10–100 ng of previously isolated mRNA (from species with polyA tails)
  • Walkaway automation—minimize hands-on time and increase consistency using Apollo scripts developed specifically for running TruSeq Stranded mRNA library prep kits

Automated VeriSeq Library Prep for PGS on the Apollo System (download protocol)

  • Precision automation for PGS—increase reliability, reproducibility, and efficiency of VeriSeq library prep for PGS
  • High-throughput capability—generate up to 96 VeriSeq libraries in 13 hours
  • Walkaway workflow—save up to ~6 hours of hands-on time

More Information

Applications

  • SMART-Seq v4 Reagent Kit for the Apollo System
    • High-throughput cDNA preparation from low-input samples consisting of 1–1,000 cells or 10 pg–10 ng of total RNA
    • mRNA-seq on Illumina or Ion Torrent platforms
  • ThruPLEX DNA Library Prep on the Apollo System
    • High-throughput, Illumina-ready NGS library preparation from 50 pg–30 ng of fragmented double-stranded DNA (gDNA, FFPE DNA, cDNA, or ChIP DNA) or 1–30 ng of cfDNA
    • DNA-seq and Plasma-seq on Illumina platforms
  • Automated TruSeq Stranded mRNA Library Prep Workflow on the Apollo System
    • Low- to high-throughput, Illumina-ready NGS library preparation with TruSeq technology using a validated Apollo script set and protocol
    • Whole-transcriptome sequencing on Illumina platforms
  • Automated VeriSeq Library Prep for PGS on the Apollo System
    • Low- to high-throughput preparation of Illumina-ready VeriSeq libraries using a validated Apollo script set and protocol
    • Screen all 24 chromosomes for aneuploidy in a single NGS assay on Illumina platforms

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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