Apollo NGS library prep system
Next-generation sequencing applications enable researchers to delve deeper into the human genome. Although NGS applications allow diverse and detailed analysis, NGS library preparation is encumbered by extensive hands-on and labor-intensive workflows. To address this, the Apollo system provides a complete, walkaway solution using validated protocols and chemistries that produce consistent libraries for DNA-seq, RNA-seq, and ChIP-seq applications.
Next-generation sequencing applications enable researchers to delve deeper into the human genome. Although NGS applications allow diverse and detailed analysis, NGS library preparation is encumbered by extensive hands-on and labor-intensive workflows. To address this, the Apollo system provides a complete, walkaway solution using validated protocols and chemistries that produce consistent libraries for DNA-seq, RNA-seq, and ChIP-seq applications.
Simplify NGS library prep with a flexible, streamlined workflow
The Apollo system offers best-in-class performance and flexibility. Manual NGS library prep workflows are labor-intensive and prevent you from conducting other valuable experiments. The Apollo system frees you from manual steps by automating the critical portions of the workflow, reducing your hands-on time to just 30 minutes, depending on the sample throughput and script used.
The Apollo system can also run flexible batch sizes without wasting reagents or time. In addition to both DNA and RNA NGS library prep protocols, this versatile system efficiently performs related protocols for sample preparation and cleanup, such as poly A+ RNA enrichment, rRNA depletion, and PCR cleanup.
Maximize yield and consistency with automated processing and validated protocols
Manual NGS library preparation is not only time-consuming, but it is also subject to human error, affecting the quality of your data. Consistency is key when processing multiple samples; the Apollo system, combined with PrepX chemistries and scripts, allows you to create consistent target libraries without the variability caused by manual pipetting. Protocols using PrepX kits on the Apollo system have been validated as a seamless solution for consistent sample preparation, cleanup, and NGS library prep. An adapted protocol for automating SMART-Seq v4 ultra-low-input RNA-seq library prep applies our industry-leading chemistry to process up to 96 samples in a single working day. These protocols employ in-tip bead separation for efficient capture of libraries in a highly reproducible manner.
Overview
Convenient library prep—reduce hands-on time with PrepX or SMART-Seq v4 chemistries and walkaway automation
Flexible throughput—run any batch size up to 96 samples per run
Unique in-tip bead separation—increase sample recovery and reproducibility
Reliable results—increase reproducibility and minimize contamination with a completely enclosed, automated system
More Information
| Table I. Validated reagent kits for the Apollo system | |
|---|---|
| Cat. # | Apollo reagent kit |
| 6401701 | SMART-Seq v4 Reagent Kit for the Apollo System |
| 6401611 | PrepX ILMN DNA Library Kit - A |
| 6401621 | PrepX ILMN DNA Library Kit - B |
| 640096 | PrepX RNA-Seq for Illumina Library Kit, 24 Samples |
| 640097 | PrepX RNA-Seq for Illumina Library Kit, 48 Samples |
| 640098 | PrepX PolyA mRNA Isolation Kit, 96 Samples |
| 6400992 | PrepX Small RNA for Illumina Library Kit, 24 Samples |
| 6401002 | PrepX Small RNA for Illumina Library Kit, 48 Samples |
- Cat. # 640170 provides enough reagent to run 96 samples in batches of up to 32 samples. Cat. # 640161 provides enough reagent to run 24 samples in batches of up to 8 samples. Cat. # 640162 provides enough reagent to run 96 samples in batches of up to 32 samples.
- Product availability varies by region.
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
See how scientists use the Apollo system!
Our collaborators and customers are constantly making scientific breakthroughs. Take a look at their published results, obtained using the Apollo Library Prep System.
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