- Long ssDNA for knockins
- Genome-wide sgRNA library system
- Recombinant Cas9 protein
- Mutation detection kits
- SNP screening
- In vitro transcription and screening kits
- Cas9-sgRNA gesicle production
- Cas9 antibodies
- Plasmid systems
- Lentiviral systems
- AAV systems
- RNA transfection
- Genotype confirmation kit
- Indel identification kit
- Cre recombinase
Guide-it SNP Screening Kit
The Guide-it SNP Screening Kit enables detection of single-nucleotide substitutions in cell populations edited using technologies such as the CRISPR/Cas9 system, providing the ability to rapidly identify edited clones from 96-well plates. The fast and simple workflow comprises PCR amplification of the genomic target site followed by an enzymatic assay using a structure-specific endonuclease that generates a fluorescent readout.
The Guide-it SNP Screening Kit enables detection of single-nucleotide substitutions in cell populations edited using technologies such as the CRISPR/Cas9 system, providing the ability to rapidly identify edited clones from 96-well plates. The fast and simple workflow comprises PCR amplification of the genomic target site followed by an enzymatic assay using a structure-specific endonuclease that generates a fluorescent readout. This method performs comparably regardless of the nucleotide substitution being assayed, the zygosity of the clone, or the sequence of the targeted locus.
One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites to mimic single-nucleotide polymorphisms (SNPs) related to human diseases, or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype. The Guide-it SNP Screening Kit addresses this need and makes the screening of hundreds of clones for single-nucleotide edits a breeze.
- High-throughput SNP screening without the need for sequencing
- A simple and rapid workflow that takes just four hours to complete
- Can be used to detect any nucleotide substitution at any genomic locus
Identifies edited clones regardless of their zygosity
- Rapid detection of single-nucleotide substitutions in edited cell populations
- SNP genotyping
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Beta testing opportunity
Takara Bio is currently developing a dual-color version of this kit that employs two Guide-it Flap Detectors in tandem (yielding red and green fluorescent signals) to enable:
- Simultaneous detection of either of two bases at the same position OR
- Detection of longer insertions.
If you are located in North America or Europe and interested in being a beta tester for this updated product, please email our technical support team at techUS@takarabio.com using the subject line: Beta tester opportunity: Guide-it SNP/Knockin Screening Kit.
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