- Long ssDNA for knockins
- Knockin screening kit
- Genome-wide sgRNA library system
- Recombinant Cas9 protein
- GMP recombinant Cas9
- Mutation detection kits
- In vitro transcription and screening kits
- Cas9-sgRNA gesicle production
- Cas9 antibodies
- Plasmid systems
- Lentiviral systems
- AAV systems
- RNA transfection
- Genotype confirmation kit
- Indel identification kit
- SNP screening
- Cre recombinase
GMP recombinant Cas9
Recombinant Cas9 Protein GMP grade is a wild-type S. pyogenes Cas9 nuclease fused with a C-terminal NLS for use in gene editing experiments. It was designed without his-tag sequences, making it appropriate for use in various applications.
This reagent is manufactured as a quality-assured product according to relevant guidelines for Good Manufacturing Practice (GMP) and is suitable for ex vivo cell engineering and gene therapy studies. Please complete and return this form prior to purchase.
Recombinant Cas9 Protein GMP grade, is manufactured and tested in accordance with the following guidelines: "Standards for Manufacturing Control and Quality Control, etc. of Investigational Products (Investigational products GMP)," as notified by the Ministry of Health and Welfare Japan ("PFSB Notification No. 0709002, July 9, 2008)"; PFSB = Pharmaceutical and Food Safety Bureau).
- No animal- or human-derived components are used in the manufacture of this product
- No his-tag sequence is in this product—appropriate for various applications; researchers recently reported a first in-human phase 1 clinical trial using engineered T cells edited by a different untagged recombinant Cas9 (Stadtmauer et al 2020)
- Appropriate for gene editing in various cell types and organisms when electroporated or microinjected as a ribonucleoprotein complex (RNP) with a single-guide RNA (sgRNA); please complete and return this form prior to purchase
Purified from Escherichia coli expressing recombinant wild-type Streptococcus pyogenes Cas9 nuclease with a C-terminal nuclear localization signal (NLS)
≥90% by HPLC
Store at or below –70°C
0.6 mg protein/0.2 ml in a 1-ml cryovial
Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 337, 816 (2012).
Liang, X. et al. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J. Biotechnol. 208, 44 (2015).
Sander, J.D. & Joung, J.K. CRISPR-Cas9 systems for genomic editing, regulation and targeting. Nat. Biotechnol. 32, 347 (2014).
Stadtmauer, E.A. et al. CRISPR-engineered T cells in patients with refractory cancer. Science. 367, eaba7365 (2020).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
RetroNectin Recombinant Human Fibronectin Fragment and RetroNectin GMP grade have widespread use as enhancers of retroviral or lentiviral gene transfer into hard-to-transduce cell types. Here, we present data on an additional application—the improved expansion of T cells from PBMCs and increased production of naïve T cells—using a combination of our products: GT-T551 T-cell medium, RetroNectin reagent, CultiLife culture bags, and anti-CD3 antibody.T-cell expansion tech note Related products
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