GMP recombinant Cas9

Recombinant Cas9 Protein GMP grade, is manufactured and tested in accordance with the following guidelines: "Standards for Manufacturing Control and Quality Control, etc. of Investigational Products (Investigational products GMP)," as notified by the Ministry of Health and Welfare Japan ("PFSB Notification No. 0709002, July 9, 2008)"; PFSB = Pharmaceutical and Food Safety Bureau). 

• No animal- or human-derived components are used in the manufacture of this product
• No his-tag sequence is in this product—appropriate for various applications; researchers recently reported a first in-human phase 1 clinical trial using engineered T cells edited by a different untagged recombinant Cas9 (Stadtmauer et al 2020)
• Appropriate for gene editing in various cell types and organisms when electroporated or microinjected as a ribonucleoprotein complex (RNP) with a single-guide RNA (sgRNA); please complete and return this form prior to purchase

Applications

Gene editing

References

Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science. 337, 816 (2012).

Liang, X. et al. Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J. Biotechnol. 208, 44 (2015).

Sander, J.D. & Joung, J.K. CRISPR-Cas9 systems for genomic editing, regulation and targeting. Nat. Biotechnol. 32, 347 (2014).

Stadtmauer, E.A. et al. CRISPR-engineered T cells in patients with refractory cancer. Science. 367, eaba7365 (2020).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.