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Rapid quantification of human cytokines using a simple, 10-min assay

Cytokine quantification is often an important step in elucidating immunological processes or assessing immune cell phenotypes. While ELISA-based tests have provided a reliable method for measuring cytokines, these approaches typically take several hours to complete and are not amenable to real-time analyses. By contrast, GoStix Plus assays enable accurate cytokine quantitation in ~10 minutes, providing reproducibility comparable to an ELISA in a fraction of the time.

GoStix Plus assays combine a lateral flow-based detection method with an accompanying smartphone app to enable rapid quantitation of human cytokines from cell culture supernatants or serum samples. The GoStix Plus protocol is simple: apply 20 μl of sample and 80 μl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette with the GoStix Plus app to quantify the amount of cytokine present in the sample. Inter-assay variability (%CV) for GoStix Plus tests is typically <15%. For information about the sensitivity and dynamic range of a given assay, please refer to the corresponding product's Certificate of Analysis.

iOS and Android versions of the GoStix Plus smartphone application are available for download from the App Store® and on Google Play™, respectively.

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Cat. #	Product	Size	Price
631285	IFN-gamma GoStix™ Plus	20 Tests	USD $470.00
IFN-γ GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human IFN-γ from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of IFN-γ present in the sample. The dynamic range and reproducibility of IFN-γ GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads, CD3/CD28 tetramer complexes, or PHA (10 μg/ml). Supernatant samples were collected at the indicated timepoints and assayed for IFN-γ protein using IFN-γ GoStix Plus or an IFN-γ ELISA. Assay results were converted into IU/ml values using NIBSC reference standard 82/587. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.
631286	IFN-gamma GoStix™ Plus	50 Tests	USD $965.00
IFN-γ GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human IFN-γ from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of IFN-γ present in the sample. The dynamic range and reproducibility of IFN-γ GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads, CD3/CD28 tetramer complexes, or PHA (10 μg/ml). Supernatant samples were collected at the indicated timepoints and assayed for IFN-γ protein using IFN-γ GoStix Plus or an IFN-γ ELISA. Assay results were converted into IU/ml values using NIBSC reference standard 82/587. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.
631283	IL-2 GoStix™ Plus	20 Tests	USD $470.00
IL-2 GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human IL-2 from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of IL-2 present in the sample. The dynamic range and reproducibility of IL-2 GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads or CD3/CD28 tetramer complexes following the manufacturers’ protocols. Supernatant samples were collected at the indicated timepoints and assayed for IL-2 protein using IL-2 GoStix Plus or an IL-2 ELISA. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.
631284	IL-2 GoStix™ Plus	50 Tests	USD $965.00
IL-2 GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human IL-2 from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of IL-2 present in the sample. The dynamic range and reproducibility of IL-2 GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads or CD3/CD28 tetramer complexes following the manufacturers’ protocols. Supernatant samples were collected at the indicated timepoints and assayed for IL-2 protein using IL-2 GoStix Plus or an IL-2 ELISA. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.
631287	TNF-alpha GoStix™ Plus	20 Tests	USD $470.00
TNF-α GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human TNF-α from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of TNF-α present in the sample. The dynamic range and reproducibility of TNF-α GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads or CD3/CD28 tetramer complexes following manufacturers’ protocols. Supernatant samples were collected at the indicated timepoints and assayed for TNF-α protein using TNF-α GoStix Plus or a TNF-α ELISA. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.
631288	TNF-alpha GoStix™ Plus	50 Tests	USD $965.00
TNF-α GoStix Plus employ a lateral flow-based detection method and accompanying smartphone application to enable rapid quantification of human TNF-α from cell culture supernatant or serum samples in only 10 minutes. Simply apply 20 µl of sample and 80 µl of chase buffer to a GoStix cassette, wait 10 minutes for test and control bands on the cassette to develop, and then scan the cassette using a mobile device running the GoStix Plus app to quantify the amount of TNF-α present in the sample. The dynamic range, and reproducibility of TNF-α GoStix Plus are comparable to the performance of an ELISA, such that users can expect to obtain similar results in a fraction of the time. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back Timelines associated with commonly used methods for measuring cytokines. Timelines associated with commonly used methods for measuring cytokines. GoStix Plus: a lateral flow-based quantitation method. Fast ELISA: measurement using targeted, preformulated ELISA reagents. Standard ELISA: measurement using standard direct or indirect sandwich assay. Bead array: multiparametric measurement by flow cytometry of bead-based, immunocapture of cytokines. Glass array: multianalyte measurement using quantitative, glass-slide multiplex ELISA microarray platform. Assay durations were taken from manufacturers’ protocols. Back GoStix Plus assays provide results similar to ELISA. GoStix Plus assays provide results similar to ELISA. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatant was collected at the time points indicated post-activation and analyzed using both IL-2 GoStix Plus and an ELISA. For the ELISA samples, several dilutions of each sample were run on the assay as suggested by the manufacturer. Back Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods. Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods. Human primary T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative selection. T cells were subsequently activated with either CD3/CD28-coated beads or CD3/CD28 tetramer complexes following manufacturers’ protocols. Supernatant samples were collected at the indicated timepoints and assayed for TNF-α protein using TNF-α GoStix Plus or a TNF-α ELISA. Back Cytokine GoStix Plus workflow. Cytokine GoStix Plus workflow. Apply 20 µl of sample to a GoStix lateral flow cassette followed by 80 µl of chase buffer. Wait 10 minutes for the test to run, then scan the cassette using the GoStix Plus smartphone app. Back Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays. Purified, human primary CD3+ T cells were activated for 24 hours using tetrameric antibody complexes to cell-surface ligands CD3, CD28, and CD2. Cell culture supernatants were collected at the indicated timepoints post activation and analyzed using IL-2, IFN-γ, and TNF-α GoStix Plus assays and the accompanying GoStix Plus app.

Overview

Rapidly quantify human cytokines from cell culture supernatant or serum samples using a simple, 10-minute assay that performs comparably to an ELISA
Obtain accurate, reproducible results without extensive technical expertise or expensive equipment
Conserve resources by identifying the most promising candidates for further analysis
Accelerate your research by obtaining results immediately rather than waiting days or weeks to accumulate enough samples for processing in high-throughput formats

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Applications

Quantification of human cytokines from cell culture supernatant or serum samples
Screening or monitoring of immune cell populations
Analysis of immune responses
Determination of suitable dilution factors for subsequent analyses

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2021 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Rapid quantification of human cytokines using a simple, 10-min assay

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using IFN-γ GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using IL-2 GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Notice to purchaser

Timelines associated with commonly used methods for measuring cytokines.

GoStix Plus assays provide results similar to ELISA.

Comparison of T-cell activation methods using TNF-α GoStix Plus and ELISA-based methods.

Cytokine GoStix Plus workflow.

Quantitation of human TH1 cytokines using GoStix Plus lateral flow assays.

Overview

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