The success of downstream applications, such as cloning or next-generation sequencing, heavily relies on the accuracy of the upstream PCR amplification step. Our PrimeSTAR series has been shown to work on intricate sequences that are highly polymorphic, homologous, or repetitive. The newly released PrimeSTAR LongSeq DNA Polymerase has the world's highest level of specificity, making it possible to amplify ultra-long chains of over 50 kb, even with GC/AT-rich sequences.

Secondary structures can impede the progression of DNA polymerase during PCR, leading to the production of truncated products. PrimeSTAR GXL DNA Polymerase is specially designed to enhance processivity and fidelity, allowing the generation of amplicons up to 30 kb long. See our technical note for details on the amplification of GC-rich templates with PrimeSTAR GXL DNA Polymerase.

When working with samples from tissue biopsies, plants, the environment, or food, it's common to encounter compounds that inhibit PCR. The Takara Ex Taq series has been proven to effectively tolerate inhibitors. Additionally, our Terra PCR Direct series enables PCR directly from tissue, crude DNA extracts, or whole blood samples.

Specially engineered for high sensitivity, Titanium Taq is generally recommended for multiplex PCR and robustly suited for a wide range of sample types. For exceptionally high-fidelity amplification, try our Advantage 2 Polymerase Mix, which has an additional proofreading polymerase. If you need a reliable, time-saving option with high-yield results, our Superplex Premix conveniently combines all the necessary reagents—just add your primers, sample, and water!

We have multiple mixes and kits for qPCR that utilize our powerful Ex Taq DNA polymerase. These have been specially designed to give sensitive, efficient detection using either probes or green intercalating dyes. Our dye-based kits utilize our proprietary TB Green dye for detection. 

PrimeScript reverse transcriptase (RT) is a powerful enzyme with strong strand-displacement activity that can overcome complex secondary structures and GC-rich targets. We offer it in three formats: a master mix for convenience, a kit with separate components for optimization, and a kit with gDNA Eraser in case of purification challenges.

Contact Customer Service for help.