Trust your cloning and sequencing data. Many downstream applications such as cloning or next-generation sequencing depend on the accuracy of the upstream PCR amplification step. Our PrimeSTAR series and Takara LA Taq polymerases have been shown to work on highly polymorphic, homologous, or repetitive sequences.

• Jia, H., Guo, Y., Zhao, W. & Wang, K. Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer. Sci. Rep. 4, 5737 (2015). (see case study)
• Hommelsheim, C. M., Frantzeskakis, L., Huang, M. & Ülker, B. PCR amplification of repetitive DNA: a limitation to genome editing technologies and many other applications. Sci. Rep. 4, 5052 (2015).
• Zhang, W., Cui, H. & Wong, L.-J. C. Comprehensive One-Step Molecular Analyses of Mitochondrial Genome by Massively Parallel Sequencing. Clin. Chem. 58, 1322–1331 (2012).

Tame your most difficult template. Secondary structure hinders the progression of DNA polymerase during PCR, which can result in truncated products. PrimeSTAR GXL DNA Polymerase and Takara LA Taq with GC buffer are specially formulated for increased processivity and fidelity to produce amplicons up to 30 kb long.

• PrimeSTAR GXL enzyme was able to efficiently amplify a GC-rich template without additives or special reaction conditions (see technical note).
• Zhu, X. J. et al. Guanine-rich sequences inhibit proofreading DNA polymerases. Sci. Rep. 6, 28769 (2016).

Capture your target from the dirtiest surroundings. Samples from tissue biopsies, plants, the environment, and food contain compounds that inhibit PCR. The Takara Ex Taq series has been proven to tolerate inhibitors from these samples. Our Terra PCR Direct series enables PCR directly from tissue, crude DNA extracts, or whole blood samples.

• Our in-house data show that Terra direct polymerase works on various food samples (including raw meat), as well as samples from human nail and whole blood, as described in the respective technical notes.
• Klos, K. L. E., Vásquez-Siller, L. M., Wetzel, H. C. & Murray, T. D. PCR-Based Detection of Cephalosporium gramineum in Winter Wheat. Plant Dis. 96, 437–442 (2012).

Amplify multiple targets with ease. Titanium Taq is specifically engineered for high-yield PCR. Its sensitivity and robustness make it ideal for multiplex PCR. In fact, several commercially available multiplex PCR assays recommend the use of Titanium Taq. For added fidelity, use Advantage 2 Polymerase Mix. For convenience, use SuperPlex Premix.

• Titanium Taq has been used successfully in high-throughput genotyping with MassARRAY (see technical note).
• Affymetrix recommends Titanium DNA Amplification Kits for use with its Genome-Wide Human SNP and CytoScan Arrays.

Get your experiment started right. Sometimes, samples are limited, degraded, or need to be assessed across a large number of targets. Prelude PreAmp Master Mix enables you to amplify your starting material up to 200X without introducing bias.

• Prelude PreAmp Master Mix outperforms all other preamplification mixes (see technical note).

Prepare full-length cDNA from diverse RNA templates. PrimeScript Reverse Transcriptase is a powerful enzyme with strong strand-displacement activity that can overcome complex secondary structures and GC-rich targets. We offer it in three formats: a master mix for convenience, a kit with separate components for optimization, and a kit with gDNA Eraser in case of purification challenges.

• Browse our technical brochure to learn more about PrimeScript RT.

Quantitate your gene expression rapidly and accurately. We have multiple mixes and kits for qPCR that utilize our powerful Ex Taq DNA polymerase. These have been specially designed to give sensitive, efficient detection using either probes or green intercalating dyes. Our dye-based kits utilize our proprietary TB Green dye for detection.

Contact your Territory Manager or inside_sales@takarabio.com for help.