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Overcome restriction site limitations in lentiviral vectors

The situation: researchers at the University of Texas Medical Branch needed to clone five different PCR fragments into a lentiviral vector that had only one restriction site suitable for subcloning.

The approach: the team used In-Fusion Cloning to choose their cloning locus based on experimental preference, instead of being limited to the one available restriction site.

The result: final vectors were complete in just three days, with only two to three hours of hands-on time required per day.

The benefit: In-Fusion Cloning allowed the team to avoid traditional ligation methods, which would have been difficult and time-consuming to perform.