We use cookies to improve your browsing experience and provide meaningful content. Read our cookie policy. Accept
  •  Customer Login
  • Register
  •  View Cart (0)
  •  Customer Login
  • Register
  •  View Cart (0)

  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us

Close

  • ‹ Back to Customer stories
  • Joining the fight against antibiotic resistance
  • Overcoming technical challenges in extracellular vesicle research
  • Sequencing grey matter
  • Unlocking cardiomyocyte heterogeneity: the role of transcription factors
  • Profiling transcription factors with CUT&RUN sequencing
PrimeSTAR GXL Premix
SMARTer NGS Single-cell RNA-seq products
Home › About › BioView blog › Customer stories › Unlocking cardiomyocyte heterogeneity: the role of transcription factors

BioView blog

  • Automation
    • Takara Bio Single-Cell Workshop, Spring 2021
    • Big problems from small bugs
    • Better biobanking with high-throughput qPCR
    • Top 5 considerations when automating single-cell sequencing
    • What's inside automated single-cell RNA-seq platforms?
  • Cancer research
    • When Epstein-Barr virus becomes a chronic menace
    • Advancing cancer research with plasma-seq
    • Amplifying our understanding of breast cancer metastases
    • Cancer immunotherapy
    • Accurate detection of SNVs and CNVs from 5-cell inputs
  • Career spotlights
    • Career spotlight: senior inside sales representative
    • Career spotlight: manufacturing engineer
    • Career spotlight: senior lead development representative
    • Career spotlight: technical support scientist
    • Career spotlight: technical writer
  • Current events
    • Your spit can save your life
    • Controlling the spread of COVID-19 with direct saliva testing
    • The 2020 Nobel Prize in Chemistry for CRISPR
    • In memory of Hermann Bujard
    • Coronavirus publication: know your enemy
    • Dethroning king coronavirus with novel vaccines
    • Entering new worlds while staying in place
    • Working hard to meet production demands for SARS-CoV-2 testing
    • Shelter in place
    • Takara Bio plays a crucial role in fighting the novel coronavirus
    • Screening for novel coronavirus with one-step RT-qPCR: getting ahead of the outbreak
  • Customer stories
    • Joining the fight against antibiotic resistance
    • Overcoming technical challenges in extracellular vesicle research
    • Sequencing grey matter
    • Unlocking cardiomyocyte heterogeneity: the role of transcription factors
    • Profiling transcription factors with CUT&RUN sequencing
  • Gene editing
    • Measure twice, cut once
    • Successful knockout experiments part II
    • Successful knockout experiments part I
    • Efficient nonviral T-cell engineering using CRISPR
  • Research news
    • TCR-seq methods: strengths, weaknesses, and rankings
    • A faster path to analysis for mAbs as therapeutic agents
    • Gene therapy takes a step forward
    • ICELL8 technology keeps cardiovascular research pumping
    • Women's Networking Event at AGBT 2020
    • Detecting infectious disease threats in a changing climate
    • Unraveling the world of microorganisms
    • Microbiomes in the brain and belly
    • Gaining insight into pulmonary arterial hypertension with purified exosomes
    • Total RNA-seq from human biofluids and EVs
    • Seq-ing the small
    • Taking the SMARTer approach to RNA-seq of FFPE tissues
  • Single-cell analysis
    • ABRF publishes single-cell RNA-seq benchmarking study
    • Two RNA-seq approaches reveal resident memory T cells hold the power to reverse liver fibrosis
    • Combination of single-cell RNA-seq approaches yields insights into the brain
    • Combining droplet and full-length sequencing technologies for a complete picture
    • Smashing single-cell sequencing sensitivity
    • Change of heart: exploring transcriptional variation in cardiomyocytes
    • Accelerating chromatin mapping with single-cell ATAC-seq
    • Bringing epigenomic profiling to the single-cell biology stage
    • Using the power of RNA-seq to characterize brain cell types
  • Stem cell research
    • Expert advice for hESC-based cell therapy development
    • Tools for iPSC-derived disease model development
    • 20 years of human stem cell research
    • Choosing a CMO partner for stem cell therapy manufacturing
    • Maximize transduction efficiency in hematopoietic cells
  • Tips and troubleshooting
    • Best practices for RNA-seq: Optimizing sample prep
    • Your quest for a better diagnostic assay development partner ends here
    • Designing primers for site-directed mutagenesis
    • Qualities to look for in your ideal OEM partner
    • Understanding viral titration—behind the science
    • 4 factors to consider for immune repertoire profiling
    • 5 FACS tips for scRNA-seq
    • Choosing a his-tagged purification resin
    • 5 tips to make your single-cell RNA-seq experiments a success
    • Using UMIs in NGS experiments
    • Web and mobile apps
    • One-step vs. two-step RT-qPCR
    • Avoid DNA contamination in PCR
    • When your his-tagged constructs don't bind
  • Women in STEM
    • Women in Science Day 2022
    • Women in STEM interview: Christina Chang
    • Women in STEM interview: Kim Smith
  • That's Good Support!
  • About our blog
Need help?
Contact Sales
PrimeSTAR GXL Premix
SMARTer NGS Single-cell RNA-seq products

Unlocking cardiomyocyte heterogeneity: the role of transcription factors

Date: April 29, 2019

Author: Takara Bio Blog Team

Categories: NGS | Stem Cells | PCR | Single-cell

Meeting the challenges of heart disease with stem cell research

Cardiovascular disease has a significant impact on global health and the world economy (Benjamin et al. 2017). For example, heart failures such as strokes or infarcts can cause irreversible damage to cardiac tissue and are potentially deadly medical emergencies. Therefore, researchers are working to characterize cardiomyocytes (which are involved in the restoration of cardiac tissue) as part of the ongoing effort to develop tools for treating heart disease. We discussed some of the methods and challenges of cardiomyocyte research with Jared Churko, PhD, who is currently the Director of the iPSC Core and an Assistant Professor in the Department of Cellular and Molecular Medicine at the University of Arizona. His lab is studying the mechanisms of heart disease by utilizing human induced pluripotent stem cells (hiPSC) to generate cardiac cell types.

In a recent article, Jared Churko and colleagues studied the hiPSC-cardiomyocyte transcriptome and identified different cardiomyocyte subpopulations based on RNA-seq single-cell analysis (Churko et al. 2018). To better understand these subpopulations, the authors knocked out transcriptional regulators using CRISPR technology and identified three transcription factors involved in hiPSC-cardiomyocyte differentiation. These transcription factors could potentially be used to enrich for cardiomyocytes in specific developmental stages with the desired functional characteristics needed to treat various cardiac diseases.

Dr. Churko visited Takara Bio recently and we sat down with him to talk about his research, as well as the benefits and limitations of single-cell RNA-seq as applied to hiPSC-derived stem cell analysis. Here are some excepts from our conversation.

Can you describe your project?

My research focuses on how heart cells—the cells that keep your heart beating—are different from each other and how we can generate them in culture. In the future, we hope to use these new heart cells to heal damaged hearts in people who have an underlying heart condition.

There are different types of specialized heart cells. How does your research show what’s happening in the model system you are using?

Certain diseases only affect certain varieties of heart cells, so it’s important to be able to make different types of heart cells. For example, if you have an infarct or ischemia in your ventricular cells, you want to make sure you use ventricular cells to repair the resulting damage. This is important because if you add an electrical-type cell like a nodal cell instead, you will cause irregular beating in the ventricular region of the heart. My research is focused on making sure we’re engineering new heart cells that are properly tuned for the area of the heart where the disease is occurring, so that in the future they can be used to attempt to cure the disease.

Figure 1. Identification of hiPSC-CM subpopulations by single-cell RNA-seq. A monolayer cardiomyocyte differentiation protocol was used to assess how single-cell populations change over time. Single-cell RNA-seq was performed on Day 0, Day 5, Day 14, and Day 45 of differentiation and 10,376 cell states were identified using Iterative Clustering and Guide-gene Selection (ICGS) and Seurat-canonical correlation analysis (CCA) software. A t-SNE plot was generated using AltAnalyze to visualize the expression of ICGS-associated markers during the different days of differentiation. Twenty distinct cell populations were observed and could be attributed to populations representing pluripotent stem cells (PSC), definitive endoderm (DE), mesoderm (MESO), ectoderm (ECTO), stromal, neural crest (NC), endothelial cells, and early, mid or late cardiomyocyte progenitors. Image adapted from Fig. 1c of Churko et al. 2018, which can be accessed here, and used under Creative Commons Attribution 4.0 International license.

Why is single-cell RNA seq important for your work?

It’s due to the resolution that you get. Although you get a lot of data from bulk sequencing, single-cell RNA seq allows you to distinguish between different subtypes and to look at different transcripts at a single-cell level, versus compiling all your data using a bulk RNA-seq method. With a single-cell method, a single population of cells that you are interested in can be isolated and analyzed, keeping other cell populations for different projects or different kinds of studies. This is of major importance, because if a certain cancer cell occurs at a frequency of 0.001% in a population of cells, that wouldn't be detected using bulk sequencing. But if single-cell sequencing is performed on that particular cell, its specific gene expression profile can be analyzed and targeted therapeutics can be developed, versus getting more noise from the other cells that you’ve also sequenced using bulk methods.

What are the limitations of the technologies used in your work?

The biggest limitation is adding understanding to the data—being able to say what it means. For example, "What's important?", "What genes are important?", and "What are you using as your reference?" The biggest concern is not whether we can get the data now, but actually acquiring enough background knowledge to make sense of it, so we can use it downstream.


We are excited to learn how innovative methods are applied to generate advances in research. Jared's research confirmed CRISPR knockouts of the transcription factors with PCR using our PrimeSTAR GXL DNA Polymerase followed by Sanger sequencing. The library used for single-cell RNA-seq analysis was generated with our SMARTer Ultra Low RNA Kit for the Fluidigm C1. We are inspired to see how he used cutting-edge tools to find solutions to his research problems.


References

Benjamin, E. J. et al. Heart Disease and Stroke Statistics—2017 Update: A Report From the American Heart Association. Circulation 135, e146–e603 (2017).

Churko, J. M. et al. Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. Nat. Commun. 9, 4906 (2018).

Back to Blog Front


Single-cell analysis elucidates cardiomyocyte differentiation from human induced pluripotent stem cells

Learn how researchers used the ICELL8 Single-Cell System to develop a method for transcriptomically characterizing the differentiation of human iPSCs into cardiomyocytes.

Learn more

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2022 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Support
  • Contact us
  • Technical support
  • Customer service
  • Shipping & delivery
  • Sales
  • Feedback
Products
  • New products
  • Special offers
  • Instrument & reagent services
Learning centers
  • NGS
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
About
  • Our brands
  • Careers
  • Events
  • Blog
  • Need help?
  • Announcements
  • Quality and compliance
  • That's Good Science!
Facebook Twitter  LinkedIn

©2022 Takara Bio Inc. All Rights Reserved.

Region - North America Privacy Policy Terms and Conditions Terms of Use

Top



  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Whole genome amplification
  • Immune profiling
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • RNA extraction and analysis for real-time qPCR
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Restriction enzymes
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • Cell-free DNA purification kits
  • Microbiome
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • Transfection reagents
  • Cell biology assays
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Antibodies and ELISAs
  • Primary antibodies and ELISAs by research area
  • Fluorescent protein antibodies
  • New products
  • Special offers
  • Plasmid and nucleic acid prep promo
  • PCR samples
  • ThruPLEX HV special offer
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Customer service
  • Sales
  • Make an appointment with your sales rep
  • Shipping & delivery
  • Technical support
  • Feedback
  • Online tools
  • GoStix Plus FAQs
  • Partnering & Licensing
  • Vector information
  • Vector document overview
  • Vector document finder
Takara Bio's award-winning GMP-compliant manufacturing facility in Kusatsu, Shiga, Japan.

Partner with Takara Bio!

Takara Bio is proud to offer GMP-grade manufacturing capabilities at our award-winning facility in Kusatsu, Shiga, Japan.

  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Next-generation sequencing
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Webinars
  • cDNA synthesis
  • Real-time PCR
  • Overview
  • Reaction size guidelines
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • RNA purification kit finder
  • PCR
  • Citations
  • Selection guides
  • Technical notes
  • FAQ
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Stem cell research
  • Overview
  • Protocols
  • Technical notes
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Cell biology assays
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Expression systems
  • Antibodies and ELISA
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
Create a web account with us

Log in to enjoy additional benefits

Want to save this information?

An account with takarabio.com entitles you to extra features such as:

•  Creating and saving shopping carts
•  Keeping a list of your products of interest
•  Saving all of your favorite pages on the site*
•  Accessing restricted content

*Save favorites by clicking the star () in the top right corner of each page while you're logged in.

Create an account to get started

  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Thursdays
  • That's Good Science Thursdays (2021)
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Our history
  • Announcements
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Careers
  • Trademarks
  • License statements
  • Quality statement
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Website FAQs

That's GOOD Science!

What does it take to generate good science? Careful planning, dedicated researchers, and the right tools. At Takara Bio, we thoughtfully develop best-in-class products to tackle your most challenging research problems, and have an expert team of technical support professionals to help you along the way, all at superior value.

Explore what makes good science possible

 Customer Login
 View Cart (0)
  • Home
  • Products
  • Services & Support
  • Learning centers
  • Applications
  • About
  • Contact us
  •  Customer Login
  • Register
  •  View Cart (0)

Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).

Clontech, TaKaRa, cellartis

  • Products
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • Diagnostic solutions
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • mRNA and cDNA synthesis
  • COVID-19 research
  • Next-generation sequencing
  • RNA-seq
  • DNA-seq
  • Single-cell NGS automation
  • Bioinformatics tools
  • Whole genome amplification
  • Immune profiling
  • Epigenetics and small RNA sequencing
  • NGS accessories
  • Gene function
  • Gene editing
  • Viral transduction
  • Fluorescent proteins
  • T-cell transduction and culture
  • Tet-inducible expression systems
  • ProteoTuner protein control systems
  • iDimerize inducible protein interaction systems
  • Transfection reagents
  • Mammalian expression plasmids
  • Cell biology assays
  • Stem cell research
  • Media and supplements
  • Stem cells and stem cell-derived cells
  • Single-cell cloning of edited hiPS cells
  • Accessories
  • Protein research
  • Purification products
  • Two-hybrid and one-hybrid systems
  • Mass spectrometry reagents
  • Expression vectors & systems
  • Glycobiology
  • Antibodies and immunoprecipitation
  • SDS-PAGE & western blotting
  • Protein sequencing
  • Accessory enzymes
  • Diagnostic solutions
  • SARS-CoV-2 clinical diagnostic tests
  • PCR
  • Most popular polymerases
  • Standard PCR
  • High-yield PCR
  • High-fidelity PCR
  • Fast PCR
  • Long-range PCR
  • GC rich PCR
  • Direct PCR
  • PCR master mixes
  • Custom business friendly and automation-ready solutions
  • Molecular diagnostic products
  • GMP-grade products
  • Application-specific PCR
  • Other PCR-related products
  • PCR thermal cyclers
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
  • Mutagenesis kits
  • Ligation enzymes
  • Restriction enzymes
  • Modifying enzymes
  • X-Gal and IPTG
  • Linkers, primers, and cloning vectors
  • Agarose gel electrophoresis
  • Nucleic acid extraction
  • Nucleic acid purification
  • Plasmid purification kits
  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
  • RNA cleanup kits
  • Cell-free DNA purification kits
  • Viral DNA and RNA purification kits
  • Microbiome
  • Accessories and components
  • Antibodies and ELISA
  • Primary antibodies and ELISAs by research area
  • Secondary antibodies
  • Antibody and ELISA accessories
  • Fluorescent protein antibodies
  • Real-time PCR
  • Real-time PCR kits
  • Reverse transcription prior to qPCR
  • High-throughput qPCR solutions
  • Real-time PCR primer sets
  • References and standards for qPCR
  • RNA extraction and analysis for real-time qPCR
  • mRNA and cDNA synthesis
  • In vitro transcription
  • cDNA synthesis kits
  • Reverse transcriptases
  • RACE kits
  • Purified cDNA & genomic DNA
  • Purified total RNA and mRNA
  • cDNA synthesis accessories
  • COVID-19 research
  • Viral detection with qPCR
  • SARS-CoV-2 pseudovirus
  • Human ACE2 stable cell line
  • Viral RNA isolation
  • Viral and host sequencing
  • Vaccine development
  • CRISPR screening
  • Drug discovery
  • Immune profiling
  • Publications
  • Services & Support
  • Instrument services
  • OEM & custom enzyme manufacturing
  • Stem cell services
  • Gene and cell therapy manufacturing services
  • Customer service
  • Technical support
  • Sales
  • Shipping & delivery
  • Partnering & Licensing
  • Feedback
  • Webinars from Takara Bio
  • Vector information
  • Online tools
  • Instrument services
  • Apollo services
  • ICELL8 services
  • SmartChip services
  • OEM & custom enzyme manufacturing
  • Services
  • Quality
  • Expertise
  • OEM enzyme FAQs
  • Custom enzyme samples
  • Exploring OEM and custom enzyme partnerships
  • Stem cell services
  • Clinical-grade stem cell services
  • Research-grade stem cell services
  • Outsourcing stem cell-based disease model development
  • Gene and cell therapy manufacturing services
  • Services
  • Facilities
  • Our process
  • Resources
  • Sales
  • Make an appointment with your sales rep
  • Webinars from Takara Bio
  • NGS: biomarkers and oncology
  • NGS: immunology
  • Stem cells
  • Real-time PCR
  • Gene function
  • Protein science
  • Vector information
  • Vector document overview
  • Vector document finder
  • Online tools
  • GoStix Plus FAQs
  • Learning centers
  • Automation systems
  • Next-generation sequencing
  • Gene function
  • Stem cell research
  • Protein research
  • PCR
  • Cloning
  • Nucleic acid purification
  • Antibodies and ELISA
  • Real-time PCR
  • cDNA synthesis
  • Automation systems
  • SmartChip Real-Time PCR System introduction
  • ICELL8 introduction
  • Apollo library prep system introduction
  • Next-generation sequencing
  • Product line overview
  • Technical notes
  • Featured kits
  • Technology and application overviews
  • FAQs and tips
  • DNA-seq protocols
  • Bioinformatics resources
  • Newsletters
  • Webinars
  • Citations
  • Posters
  • Gene function
  • Gene editing
  • Viral transduction
  • T-cell transduction and culture
  • Inducible systems
  • Transfection reagents
  • Fluorescent proteins
  • Cell biology assays
  • Stem cell research
  • Overview
  • Protocols
  • Applications
  • Technical notes
  • Posters
  • Webinars
  • Videos
  • FAQs
  • Citations
  • Selection guides
  • Protein research
  • Capturem technology
  • Antibody purification and immunoprecipitation
  • His-tag purification
  • Other tag purification
  • Phosphoprotein and glycoprotein purification
  • Mass spectrometry digestion reagents
  • Matchmaker Gold yeast two-hybrid systems
  • Expression systems
  • PCR
  • Citations
  • Selection guides
  • PCR enzyme brochure
  • Technical notes
  • FAQ
  • Go green with lyophilized enzymes
  • LA PCR technology
  • Cloning
  • In-Fusion Cloning general information
  • Primer design and other tools
  • In‑Fusion Cloning tips and FAQs
  • Applications and technical notes
  • Sign up to stay updated
  • Traditional molecular cloning
  • Nucleic acid purification
  • Nucleic acid extraction webinars
  • Product demonstration videos
  • Product finder
  • Plasmid kit selection guide
  • Plasmid purification
  • Genomic DNA purification
  • DNA/RNA cleanup and extraction
  • RNA purification
  • RNA purification kit finder
  • Viral DNA and RNA purification
  • Parallel DNA, RNA & protein
  • Automated DNA and RNA purification
  • Accessory selection guides
  • Microbiome
  • Antibodies and ELISA
  • Osteocalcin focus
  • Real-time PCR
  • Overview
  • Product finder
  • Reaction size guidelines
  • Real-time PCR products brochure
  • Real-time PCR tutorial videos
  • Guest webinar: extraction-free SARS-CoV-2 detection
  • Guest webinar: developing and validating molecular diagnostic tests
  • Technical notes
  • FAQs
  • cDNA synthesis
  • Premium total and poly A+ RNA
  • SMARTer RACE 5'/3' Kit
  • Cloning antibody variable regions
  • Applications
  • Molecular diagnostics
  • Pathogen detection
  • Vaccine development
  • Cancer research
  • Immunotherapy research
  • Alzheimer's disease research
  • Reproductive health technologies
  • Molecular diagnostics
  • Applications
  • Solutions
  • Partnering
  • Webinar: Speeding up diagnostic development
  • Contact us
  • Pathogen detection
  • Sample prep
  • Detection methods
  • Identification and characterization
  • SARS-CoV-2
  • Antibiotic-resistant bacteria
  • Food crop pathogens
  • Waterborne disease outbreaks
  • Viral-induced cancer
  • Vaccine development
  • Characterizing the viral genome and host response
  • Identifying and cloning vaccine targets
  • Expressing and purifying vaccine targets
  • Immunizing mice and optimizing vaccine targets
  • Cancer research
  • Sample prep from FFPE tissue
  • Sample prep from plasma
  • Cancer biomarker discovery
  • Cancer biomarker quantification
  • Single cancer cell analysis
  • Cancer genomics and epigenomics
  • HLA typing in cancer
  • Gene editing for cancer therapy/drug discovery
  • Immunotherapy research
  • T-cell therapy
  • Antibody therapeutics
  • T-cell receptor profiling
  • TBI initiatives in cancer therapy
  • Alzheimer's disease research
  • Antibody engineering
  • Sample prep from FFPE tissue
  • Single-cell sequencing
  • Reproductive health technologies
  • Preimplantation genetic testing
  • About
  • BioView blog
  • That's Good Science!
  • Our brands
  • Our history
  • Announcements
  • Events
  • Careers
  • Trademarks
  • License statements
  • Quality and compliance
  • Takara Bio affiliates & distributors
  • Need help?
  • Website FAQs
  • DSS Takara Bio India Pvt. Ltd : Manufacturing
  • Our partners
  • Special offers
  • New products
  • BioView blog
  • Automation
  • Cancer research
  • Career spotlights
  • Current events
  • Customer stories
  • Gene editing
  • Research news
  • Single-cell analysis
  • Stem cell research
  • Tips and troubleshooting
  • Women in STEM
  • That's Good Support!
  • About our blog
  • That's Good Science!
  • DNA Day 2022
  • That's Good Science Thursdays
  • That's Good Science Thursdays (2021)
  • That's Good Science Podcast
  • Season one
  • Season two
  • Season three
  • Our brands
  • Takara
  • Clontech
  • Cellartis
  • Events
  • Biomarker discovery events
  • Calendar
  • Conferences
  • Speak with us
  • Takara Bio affiliates & distributors
  • United States and Canada
  • China
  • Japan
  • Korea
  • Europe
  • India
  • Affiliates & distributors, by country
  • Need help?
  • Privacy request
  • Special offers
  • Plasmid and nucleic acid prep promo
  • PCR samples
  • ThruPLEX HV special offer
  • Lab Essentials
  • Products
  • Services & Support
  • Learning centers
  • APPLICATIONS
  • About
  • Contact Us