Prelude PreAmp Master Mix—sensitive, unbiased preamplification

Prelude PreAmp Master Mix is a 2X PCR mix optimized for unbiased preamplification of multiple (>100) targets, starting from a limited (100 pg–10 ng) input of cDNA or gDNA. Amplified products can then be used for downstream real-time PCR (qPCR), genotyping, or target enrichment analysis.

Cat. #	Product	Size		License	Quantity	Details
638542	Prelude™ PreAmp Master Mix	10 Rxns	USD $178.00
Prelude PreAmp Master Mix uses an optimized 2X PCR mix for unbiased preamplification of over 100 targets, starting from a limited input of cDNA or gDNA (100 pg–10 ng). Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target enrichment analysis. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components Image Data Back The benefits of preamplification Figure 1. The benefits of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Data points for nonpreamplified genes with C_t >32 are not shown. Back Uniform representation following preamplification Figure 2. Uniform representation following preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Back Uniformity following either 10 or 14 cycles of preamplification Figure 3. Uniformity following either 10 or 14 cycles of preamplification. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Back Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles Figure 4. Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Back Sensitive, unbiased preamplification using as little as 12.5 pg of starting material Figure 5. Sensitive, unbiased preamplification using as little as 12.5 pg of starting material. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Back Takara PreAmp Master Mix provides less biased preamplification than other master mixes Figure 6. Takara PreAmp Master Mix provides less biased preamplification than other master mixes. Please note that although the above graph displays data for all 96 target genes in the PrimerArray Cell Cycle (Human) panel, only some of the gene names are shown on the x-axis to ensure their readability. The complete list of target genes can be found here. Back Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue Figure 7. Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue. Back TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue Figure 8. TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue. Back 638542: Prelude PreAmp Master Mix

Overview

Least-biased preamplification—obtain at least 2–3X fewer outliers than other commercially available preamplification mixes
Greatest sensitivity—start with as little as 100 pg of cDNA or gDNA
Universal compatibility—preamplified samples can be used for real-time PCR on over 100 green intercalating dye or probe-based targets and for SNP genotyping and target enrichment
Convenient, ready-to-use master mix—just add cDNA or gDNA samples and primers, and then cycle 10–14 times

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Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Achieve the most unbiased, target-specific preamplification from limited samples using Prelude PreAmp Master Mix

View data on sensitive, unbiased preamplification from limited samples.

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High-throughput real-time PCR

Where throughput meets flexibility

Real-time PCR (qPCR) is a powerful technique for genotyping and gene expression analysis. Currently, qPCR experiments are becoming increasingly complex—involving an expansive and growing list of targets from a larger number of samples, all with more technical replicates. The SmartChip Real-Time PCR System is a complete high-throughput solution that enables an unrivaled amount of flexible assay and sample formats, allowing researchers to seamlessly switch between dispensing assay reagents and samples into blank chips, or dispensing samples into custom, preprinted chips without the need for revalidation.

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Prelude PreAmp Master Mix—sensitive, unbiased preamplification

Notice to purchaser

The benefits of preamplification

Uniform representation following preamplification

Uniformity following either 10 or 14 cycles of preamplification

Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles

Sensitive, unbiased preamplification using as little as 12.5 pg of starting material

Takara PreAmp Master Mix provides less biased preamplification than other master mixes

Takara PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue

TaqMan PreAmp Master Mix displays more bias than Takara PreAmp Master Mix when assaying 162 breast cancer targets in FFPE tissue

638542: Prelude PreAmp Master Mix

Overview

More Information

Additional product information

Where throughput meets flexibility