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  • ‹ Back to Total RNA-seq
  • SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)
  • SMART-Seq Total RNA Pico Input with UMIs (ZapR Mammalian)
  • SMART-Seq Total RNA Pico Input (ZapR Mammalian)
  • SMART-Seq Total RNA Mid Input
  • SMART-Seq Total RNA High Input (RiboGone Mammalian)
  • Pico-input strand-specific MGI sequencing libraries from mammalian samples
  • SMART-Seq Stranded for MGI
RNA-seq selection guide
Technical notes View data for this product
Home › Products › Next-generation sequencing › RNA-seq › Total RNA-seq › SMART-Seq Total RNA Mid Input

RNA-seq

  • mRNA-seq
    • Long-read mRNA-seq
    • Full-length mRNA-seq
      • SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell
      • SMART-Seq mRNA LP and SMART-Seq mRNA
      • SMART-Seq mRNA HT and SMART-Seq mRNA HT LP
    • Full-length mRNA-seq and RNA counting with UMIs
    • 3’ Differential Expression
    • Target capture
  • Total RNA-seq
    • SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)
    • SMART-Seq Total RNA Pico Input with UMIs (ZapR Mammalian)
    • SMART-Seq Total RNA Pico Input (ZapR Mammalian)
    • SMART-Seq Total RNA Mid Input
    • SMART-Seq Total RNA High Input (RiboGone Mammalian)
  • RNA-seq accessories
    • Ribosomal RNA removal
    • Single-cell lysis buffer
  • Legacy RNA-seq kits
    • SMART-Seq Stranded for total RNA-seq
    • Pico-input strand-specific total RNA-seq for mammalian samples v2
    • Pico-input strand-specific total RNA-seq for mammalian samples v3
    • SMART-Seq Single Cell for scRNA-seq
    • SMARTer RNA Unique Dual Index Kits
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RNA-seq selection guide
Technical notes View data for this product

SMART-Seq Total RNA Mid Input—strand-specific library construction for whole transcriptome analysis on Illumina platforms

NOTE: SMART-Seq Total RNA Mid Input is an equivalent replacement for the SMARTer Stranded RNA-Seq Kit, SMARTer Stranded RNA-Seq Kit HT, and SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. RiboGone Mammalian for rRNA removal is not included, but can be purchased separately for use with mammalian total RNA
  2. For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
  3. New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)

SMART-Seq Total RNA Mid Input provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA and yields robust data from as little as 100 pg–100 ng of input RNA. Unique Dual Index Kits (Cat. # 634752–634756) allow for multiplexing of up to 384 Illumina-ready sequencing libraries.

NOTE: SMART-Seq Total RNA Mid Input is an equivalent replacement for the SMARTer Stranded RNA-Seq Kit, SMARTer Stranded RNA-Seq Kit HT, and SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).

The updates (listed below) do not in any way impact the protocols or functional performance of these kits.

  1. RiboGone Mammalian for rRNA removal is not included, but can be purchased separately for use with mammalian total RNA
  2. For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
  3. New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)

SMART-Seq Total RNA Mid Input provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA and yields robust data from as little as 100 pg–100 ng of input RNA.

The kit provides complete transcriptome coverage for both coding and noncoding RNA, and the incorporation of Illumina indexes and adapters is included in the workflow, eliminating the need for a downstream library preparation kit. Unique Dual Index Kits (Cat. # 634752–634756) allow for multiplexing of up to 384 Illumina-ready sequencing libraries.

For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. For integrated rRNA depletion and library preparation for mammalian samples from 250 pg–10 ng of total RNA, we recommend our SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian. For higher inputs (100 ng–1 µg of mammalian total RNA) we recommend our SMART-Seq Total RNA High Input (RiboGone Mammalian).

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Cat. # Product Size Price License Quantity Details
635048 SMART-Seq® Total RNA Mid Input 4 x 96 Rxns USD $10277.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 

SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 384 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635048: SMART-Seq Total RNA Mid Input

635048:  SMART-Seq Total RNA Mid Input

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)+ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

Required Products

Cat. # Product Size Price License Quantity Details
634846 RiboGone™ - Mammalian 6 Rxns USD $472.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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634846: RiboGone - Mammalian

634846: RiboGone - Mammalian

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).
634847 RiboGone™ - Mammalian 24 Rxns USD $1514.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

Back

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian

634847: RiboGone - Mammalian
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

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744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
635049 SMART-Seq® Total RNA Mid Input 24 Rxns USD $877.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 

SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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635049: SMART-Seq Total RNA Mid Input

635049:  SMART-Seq Total RNA Mid Input

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)+ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

Required Products

Cat. # Product Size Price License Quantity Details
634846 RiboGone™ - Mammalian 6 Rxns USD $472.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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634846: RiboGone - Mammalian

634846: RiboGone - Mammalian

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).
634847 RiboGone™ - Mammalian 24 Rxns USD $1514.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian

634847: RiboGone - Mammalian
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

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744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
635050 SMART-Seq® Total RNA Mid Input 96 Rxns USD $2843.00

License Statement

ID Number  
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 

SMART-Seq Total RNA Mid Input includes the components needed to generate indexed cDNA libraries suitable for next-generation sequencing (NGS) on any Illumina platform, starting from as little as 100 pg of poly(A)-purified or ribosomal RNA-depleted RNA. The directionality of the template-switching reaction preserves the strand orientation of the RNA, making it possible to obtain strand-specific sequencing data from the synthesized cDNA. Indexes are added using a unique dual index kit. The quantity of reagents in this size is sufficient for up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

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cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations

cDNA libraries produced using the SMARTer Stranded RNA-Seq Kit show comparable yields and purity irrespective of input RNA concentrations. Agilent 2100 Bioanalyzer gel-like image using a High Sensitivity DNA chip with cDNA libraries produced from between 100 ng–100 pg human brain poly(A)+ RNA with the number of PCR cycles indicated. cDNA libraries were produced by amplification with SeqAmp DNA Polymerase (included in the SMARTer Stranded RNA-Seq Kit). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels

The SMARTer Stranded RNA-Seq Kit provides high reproducibility and sensitivity over a 1,000-fold range of input RNA levels. Scatter plots of expression (FPKM) for cDNA libraries prepared from 100 ng and 100 pg of human brain poly(A)+ RNA show a high correlation, suggesting consistency across input levels. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations

Data from technical replicates of each library indicate that the SMARTer Stranded RNA-Seq Kit delivers very consistent results across a wide range of input RNA concentrations. Comparisons of pairs of cDNA library replicas created from 100 ng and 100 pg of input RNA show high reproducibility across a wide range of input RNA. Axes are plotted on a log10 scale. Insets indicate the Pearson coefficient of correlation between replicates (R). Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

Even at the lowest levels tested [100 pg poly(A)+ input RNA], the SMARTer Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments

The SMARTer Stranded RNA-Seq Kit maintains high accuracy even with low input volume. ~15,000 genes were detected using strand-specific alignments, even with only 100 pg poly(A)+ input RNA. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis

High reproducibility with the SMARTer Stranded RNA-Seq Kit, confirmed by ERCC analysis. Reads mapping to the ERCC data set from the previous experiments were pooled together and the FPKM was plotted against relative transcript abundance. The complete set of 92 ERCC transcripts was identified, with a slope of 0.934 and R2 of 0.9725. Axes are plotted on a log2 scale. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq Platform with 2 x 100 bp paired end reads

Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina HiSeq® Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit

Distinguishing overlapping and antisense transcripts with the SMARTer Stranded RNA-Seq Kit. Panel A. RNA-seq reads from the Human Brain Poly A+ RNA cDNA library were mapped against the human genome. The SMARTer Stranded method allowed assignment of sequencing reads to the correct gene in the case of overlapping PHC1 and M6PR transcripts. Panel B. Strand-specific coverage of the CDR1 locus. Nearly all reads are antisense to the annotated transcript, a finding independently reported elsewhere (Hansen, T. B. et al. (2011) EMBO J. 30(21):4414–4422). Panel C. Comparison of CDR1 gene counts obtained using either a strand-agnostic or strand-aware method. Data is shown for the SMARTer Stranded RNA-Seq Kit. SMART-Seq Total RNA Mid Input is an equivalent replacement.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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635050: SMART-Seq Total RNA Mid Input

635050:  SMART-Seq Total RNA Mid Input

Required Products

Cat. # Product Size Price License Quantity Details
634846 RiboGone™ - Mammalian 6 Rxns USD $472.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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634846: RiboGone - Mammalian

634846: RiboGone - Mammalian

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).
634847 RiboGone™ - Mammalian 24 Rxns USD $1514.00

License Statement

ID Number  
246
This Product is protected by one or more patents from the family consisting of: US9428794, US10421992, US11149303, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

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RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis. RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.

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Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA

Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA
Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.

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Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples

Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples
Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.

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RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA

RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA
RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10–100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).

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634847: RiboGone - Mammalian

634847: RiboGone - Mammalian
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
744970.5 NucleoMag® NGS Clean-up and Size Select 5 mL USD $113.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.5 consists of a bottle containing 5 ml of bead suspension.

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744970.5: NucleoMag NGS Clean-up and Size Select

744970.5: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.
744970.50 NucleoMag® NGS Clean-up and Size Select 50 mL USD $770.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.50 consists of a bottle containing 50 ml of bead suspension.

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

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Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

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Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

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Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

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744970.50: NucleoMag NGS Clean-up and Size Select

744970.50: NucleoMag NGS Clean-up and Size Select
744970.500 NucleoMag® NGS Clean-up and Size Select 500 mL USD $5305.00

NucleoMag NGS Clean-up and Size Select employs scalable, automation-friendly magnetic bead technology to enable efficient cleanup of DNA or RNA fragments. The flexible protocol enables processing of 50–150 µl input volumes and allows for enrichment of fragments ranging in size from 150–800 bp. NucleoMag NGS Clean-up and Size Select consists of paramagnetic beads suspended in binding buffer that selectively bind DNA and RNA fragments based on the volume ratio of bead suspension to sample. After magnetic separation and removal of supernatant, the beads are washed with ethanol followed by a short drying step. Following elution in low-salt buffer or water, the highly purified DNA is free of contaminants such as nucleotides, primers, adapters, adapter dimers, enzymes, buffer additives, or salts, and can be used directly in downstream applications. NucleoMag NGS Clean-up and Size Select provides comparable performance and employs the same dilution ratios for size selection as magnetic bead-based solutions from other providers, such that it can be incorporated in existing workflows without the need for protocol adjustment or optimization.

Cat. # 744970.500 consists of a bottle containing 500 ml of bead suspension.

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744970.500: NucleoMag NGS Clean-up and Size Select

744970.500: NucleoMag NGS Clean-up and Size Select

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Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select

Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select
Tunable selection of DNA fragments in a given size range using NucleoMag NGS Clean-up and Size Select. NucleoMag NGS Clean-up and Size Select can be used to isolate DNA fragments of a given size within a target range of ~150�800 bp. Selective binding of DNA fragments above a designated size cutoff is achieved by applying NucleoMag beads to the sample at a predetermined dilution ratio. This approach can be applied once or twice to perform single- or double-sided size selection. In the latter case, an initial round of bead purification is typically performed to bind and remove fragments above the upper size cutoff, and a second round of purification is performed to bind and retain fragments above the lower size cutoff. Dilution ratios used for size selection with NucleoMag beads are similar to ratios employed by size-selection beads from other providers, such that NucleoMag beads can be incorporated in existing NGS library preparation workflows without protocol adjustment.

Back

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select

Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select
Relationship between bead-to-sample volume ratio and recovery rate for DNA fragments of different sizes with NucleoMag NGS Clean-up and Size Select. Varying quantities of NucleoMag beads were applied to 100-?l aliquots containing mixtures of DNA fragments ranging in size from 100 bp to 1,000 bp (10 ng/?l) in the indicated dilution ratios (gray row). Recovery rates for fragments of each size are expressed as a percentages (%).

Back

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select

Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select
Examples of double-sided size selection using NucleoMag NGS Clean-up and Size Select. Sheared mouse genomic DNA was size selected with NucleoMag beads using two different pairs of dilution ratios and measured using a Bioanalyzer. Green trace: sheared mouse genomic DNA prior to size selection. Red trace: size distribution after double-sided size selection using dilution ratios of 0.4 (right) and 0.6 (left), resulting in a mean fragment size of 460 bp. Blue trace: size distribution after double-sided size selection using dilution ratios of 0.55 (right) and 0.8 (left), resulting in a mean fragment size of 340 bp.

Back

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers

Fragment size analysis for NGS libraries prepared using size-selection beads from various providers
Fragment size analysis for NGS libraries prepared using size-selection beads from various providers. NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Back

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers

Comparison of sequencing data generated from NGS libraries prepared using size-selection beads from various providers
NGS libraries were prepared from sheared E. coli DNA using the TruSeq DNA PCR-Free kit by Illumina and size selected using magnetic beads from different providers (Illumina, AMPure XP, and NucleoMag NGS Clean-up and Size Select). Panel A. Bioanalyzer trace indicating size distribution of sheared E. coli DNA (1-?g input) prior to NGS library preparation. Panel B. Bioanalyzer traces indicating size distributions of resulting NGS libraries purified using Illumina (red), AMPure XP (blue), or NucleoMag (green) magnetic beads.

Overview

  • Accurate—Identify each transcript's strand of origin with >99% accuracy.
  • Sensitive—Detect low-abundance transcripts from as little as 100 pg of input RNA. It is recommended to use rRNA-depleted or poly(A)-purified RNA.
  • Integrated with Illumina sequencing—Incorporate Illumina indexes and adapters during PCR amplification.
  • Couldn't get easier—Go from start to finish in less than 4 hours.

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

  • RNA-seq for prokaryotic or mammalian samples on Illumina platforms

  • NGS library generation that retains strand information
  • Analysis of coding and non-coding sequence information

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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