SMART-Seq Total RNA Mid Input—strand-specific library construction for whole transcriptome analysis on Illumina platforms
NOTE: SMART-Seq Total RNA Mid Input is an equivalent replacement for the SMARTer Stranded RNA-Seq Kit, SMARTer Stranded RNA-Seq Kit HT, and SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).
The updates (listed below) do not in any way impact the protocols or functional performance of these kits.
- RiboGone Mammalian for rRNA removal is not included, but can be purchased separately for use with mammalian total RNA
- For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
- New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)
SMART-Seq Total RNA Mid Input provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA and yields robust data from as little as 100 pg–100 ng of input RNA. Unique Dual Index Kits (Cat. # 634752–634756) allow for multiplexing of up to 384 Illumina-ready sequencing libraries.
NOTE: SMART-Seq Total RNA Mid Input is an equivalent replacement for the SMARTer Stranded RNA-Seq Kit, SMARTer Stranded RNA-Seq Kit HT, and SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian with minor updates (see a complete list of kits with new names and the existing kits they will replace here).
The updates (listed below) do not in any way impact the protocols or functional performance of these kits.
- RiboGone Mammalian for rRNA removal is not included, but can be purchased separately for use with mammalian total RNA
- For added flexibility, indexes are not included; a choice of indexing primers are sold separately [see Unique Dual Index (UDI) kits]
- New kit sizes match those of the UDI kits (24 rxns, 96 rxns, 384 rxns)
SMART-Seq Total RNA Mid Input provides a solution for generating Illumina sequencing libraries that retain strand information with >99% accuracy, and is recommended for use with rRNA-depleted or poly(A)-enriched samples. The kit uses a random-priming method that is compatible with eukaryotic or prokaryotic RNA and yields robust data from as little as 100 pg–100 ng of input RNA.
The kit provides complete transcriptome coverage for both coding and noncoding RNA, and the incorporation of Illumina indexes and adapters is included in the workflow, eliminating the need for a downstream library preparation kit. Unique Dual Index Kits (Cat. # 634752–634756) allow for multiplexing of up to 384 Illumina-ready sequencing libraries.
For rRNA removal from 10–100 ng of input total RNA, we recommend the RiboGone - Mammalian kit. For integrated rRNA depletion and library preparation for mammalian samples from 250 pg–10 ng of total RNA, we recommend our SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian. For higher inputs (100 ng–1 µg of mammalian total RNA) we recommend our SMART-Seq Total RNA High Input (RiboGone Mammalian).
Overview
- Accurate—Identify each transcript's strand of origin with >99% accuracy.
- Sensitive—Detect low-abundance transcripts from as little as 100 pg of input RNA. It is recommended to use rRNA-depleted or poly(A)-purified RNA.
- Integrated with Illumina sequencing—Incorporate Illumina indexes and adapters during PCR amplification.
- Couldn't get easier—Go from start to finish in less than 4 hours.
Interested in more data and FAQs about this product? Visit the NGS Learning Center.
More Information
Applications
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RNA-seq for prokaryotic or mammalian samples on Illumina platforms
- NGS library generation that retains strand information
- Analysis of coding and non-coding sequence information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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