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  • ‹ Back to Total RNA-seq
  • SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)
  • SMART-Seq Total RNA Pico Input with UMIs (ZapR Mammalian)
  • SMART-Seq Total RNA Pico Input (ZapR Mammalian)
  • SMART-Seq Total RNA Mid Input
  • SMART-Seq Total RNA High Input (RiboGone Mammalian)
  • Pico-input strand-specific MGI sequencing libraries from mammalian samples
  • SMART-Seq Stranded for MGI
RNA-seq selection guide
Technical notes NEW! Download flyer with expanded input range data
Technical notes View data for SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
Technical notes View data for FFPE samples
Home › Products › Next-generation sequencing › RNA-seq › Total RNA-seq › SMART-Seq Total RNA Pico Input (ZapR Mammalian)

RNA-seq

  • mRNA-seq
    • Long-read mRNA-seq
    • Full-length mRNA-seq
      • SMART-Seq mRNA Single Cell LP and SMART-Seq mRNA Single Cell
      • SMART-Seq mRNA LP and SMART-Seq mRNA
      • SMART-Seq mRNA HT and SMART-Seq mRNA HT LP
    • Full-length mRNA-seq and RNA counting with UMIs
    • 3’ Differential Expression
    • Target capture
  • Total RNA-seq
    • SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)
    • SMART-Seq Total RNA Pico Input with UMIs (ZapR Mammalian)
    • SMART-Seq Total RNA Pico Input (ZapR Mammalian)
    • SMART-Seq Total RNA Mid Input
    • SMART-Seq Total RNA High Input (RiboGone Mammalian)
  • RNA-seq accessories
    • Ribosomal RNA removal
    • Single-cell lysis buffer
  • Legacy RNA-seq kits
    • SMART-Seq Stranded for total RNA-seq
    • Pico-input strand-specific total RNA-seq for mammalian samples v2
    • Pico-input strand-specific total RNA-seq for mammalian samples v3
    • SMART-Seq Single Cell for scRNA-seq
    • SMARTer RNA Unique Dual Index Kits
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Contact Sales
RNA-seq selection guide
Technical notes NEW! Download flyer with expanded input range data
Technical notes View data for SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
Technical notes View data for FFPE samples

SMART-Seq Total RNA Pico Input (ZapR Mammalian)

NOTE: SMART-Seq Total RNA Pico Input (ZapR Mammalian) is a replacement for SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian with with some updates for improved performance as indicated below:

  1. Improved efficiency of ZapR rRNA depletion reagents for better removal of unwanted reads
  2. New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
  3. For added flexibility, indexes are not included in the kit. A choice of indexing primers are sold separately (see Unique Dual Index kits)

This kit can now be used for library preparation with higher input amounts—up to 1 µg of total RNA—while maintaining the same exceptional performance. See the alternative protocol below or download our new flyer for the data. 

SMART-Seq Total RNA Pico Input (ZapR Mammalian) is designed for efficient preparation of Illumina sequencing libraries from picogram input amounts (250 pg–10 ng) of total RNA. Technologies included in this product enable processing of high-quality, partially degraded, or low-quality RNA, including RNA prepared from FFPE or LCM samples.

NOTE: SMART-Seq Total RNA Pico Input (ZapR Mammalian) is a replacement for SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian with some updates for improved performance as indicated below:

  1. Improved efficiency of ZapR rRNA depletion reagents for better removal of unwanted reads
  2. New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
  3. For added flexibility, indexes are not included in the kit. A choice of indexing primers are sold separately (see Unique Dual Index kits)

This kit can now be used for library preparation with higher input amounts—up to 1 µg of total RNA—while maintaining the same exceptional performance. See the alternative protocol below or download our new flyer for the data. 

SMART-Seq Total RNA Pico Input (ZapR Mammalian) is designed for efficient preparation of Illumina sequencing libraries from picogram input amounts of total RNA. Technologies included in this product, including our proprietary SMART (Switching Mechanism at the 5′ end of RNA Template) technology, enable processing of high-quality, partially degraded, or low-quality RNA, including RNA prepared from FFPE or LCM samples. The kit features a streamlined workflow that retains strand information and includes library prep by incorporating indexes and adapters during the reverse-transcription and PCR amplification steps. The entire protocol, including library prep and purification, takes only ~6.5 hours.

Ribosomal RNA (rRNA) comprises a significant proportion (as much as 90%) of total RNA samples. Depleting these abundant transcripts from total RNA prior to library construction provides benefits by lowering sequencing costs and improving mapping statistics. However, with very low input amounts, rRNA depletion from total RNA leaves an insufficient amount of material for the preparation of a high-quality library. The workflow used in this kit incorporates a proprietary technology that depletes ribosomal cDNA (cDNA fragments originating from rRNA molecules), using probes specific to mammalian rRNA, and some mitochondrial RNA.

Libraries produced with this kit achieve a high percentage of clusters passing filter (%PF) without significant additions of PhiX, even on Illumina platforms using two-channel SBS technology such as NextSeq® and MiniSeq™, and on HiSeq® 3000/4000. This, in turn, reduces sequencing costs by yielding more biologically relevant sequencing reads per run. Data from the kit tends to identify more unique transcripts with fewer reads mapping to rRNA and mtRNA and lower duplicate rates.

For your convenience, we have outlined the differences between our low-input total-RNA seq kits below: 

  • SMART-Seq Total RNA Pico Input (ZapR Mammalian) supports an expanded input range of 250 pg–1 µg total RNA. 
  • SMART-Seq Total RNA Pico Input with UMIs (ZapR Mammalian) supports the same expanded input range of 250 pg–1 µg total RNA and incorporates UMIs in the library prep.
  • SMART-Seq Total RNA Single Cell (ZapR Mammalian) supports an input range of 1–1,000 cells or 10 pg–10 ng total RNA
 More  Less
Cat. # Product Size Price License Quantity Details
634357 SMART-Seq® Total RNA Pico Input (ZapR® Mammalian) 24 Rxns USD $1553.00

License Statement

ID Number  
425 LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 
395
This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.  Additional information may be found at https://www.takarabio.com/patents. 
450 This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

SMART-Seq Total RNA Pico Input (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–10 ng inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries.

Back

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. Gene counts (Panel A) and gene and transcript counts (Panel B) are increased for ZapR-treated libraries.

Back

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range. SMART-Seq Total RNA Pico Input (ZapR Mammalian) was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
634358 SMART-Seq® Total RNA Pico Input (ZapR® Mammalian) 96 Rxns USD $4240.00

License Statement

ID Number  
395
This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.  Additional information may be found at https://www.takarabio.com/patents. 
425 LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 
450 This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

SMART-Seq Total RNA Pico Input (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–10 ng inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 96 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries.

Back

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. Gene counts (Panel A) and gene and transcript counts (Panel B) are increased for ZapR-treated libraries.

Back

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range. SMART-Seq Total RNA Pico Input (ZapR Mammalian) was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)
634359 SMART-Seq® Total RNA Pico Input (ZapR® Mammalian) 4 x 96 Rxns Inquire for Quotation

License Statement

ID Number  
425 LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392 This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents. 
395
This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.  Additional information may be found at https://www.takarabio.com/patents. 
450 This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.
*

SMART-Seq Total RNA Pico Input (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–10 ng inputs of purified total RNA. The protocol for use with this kit was developed to work with either high- or low-quality total RNA, does not require additional rRNA removal methods or kits, and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA—typically present in high abundance following cDNA synthesis from total RNA inputs—makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA. Enough material is provided with this kit to perform up to 384 (4 x 96) reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

Back

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range

Efficient depletion of rRNA-derived cDNAs across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries.

Back

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range

Superior gene and transcript detection across a wide input range. SMART-Seq Total RNA Pico Input was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Pico Input (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. Gene counts (Panel A) and gene and transcript counts (Panel B) are increased for ZapR-treated libraries.

Back

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range

Preservation of gene abundance information across a wide input range. SMART-Seq Total RNA Pico Input (ZapR Mammalian) was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 2 x 106 paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries.

Required Products

Cat. # Product Size Price License Quantity Details
634752 Unique Dual Index Kit (1–96) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634752: Unique Dual Index Kit (1-96)

634752: Unique Dual Index Kit (1-96)
634753 Unique Dual Index Kit (97–192) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634753: Unique Dual Index Kit (97-192)

634753: Unique Dual Index Kit (97-192)
634754 Unique Dual Index Kit (193–288) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634754: Unique Dual Index Kit (193-288)

634754: Unique Dual Index Kit (193-288)
634755 Unique Dual Index Kit (289–384) 96 Rxns USD $623.00

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

Documents Components Image Data

Back

634755: Unique Dual Index Kit (289-384)

634755: Unique Dual Index Kit (289-384)
634756 Unique Dual Index Kit (1–24) 24 Rxns USD $237.00

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

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634756: Unique Dual Index Kit (1-24)

634756: Unique Dual Index Kit (1-24)

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

  • Accommodates picogram inputs of starting material—start from 250 pg–10 ng of total RNA from human, mouse, or rat.
  • Versatile—generate reproducible data from RNA of any quality (including FFPE and LCM samples and cell-free RNA).
  • Improved sequencing performance—achieve a high percentage of clusters passing filter (%PF) without adding PhiX and identify more transcripts with fewer duplicates.
  • Strand information—identify each transcript’s strand of origin with very high accuracy.
  • Fast, streamlined protocol—go from start to finish in ~6.5 hours and save time during library purification with an improved buffer formulation.
  • Seamless integration with Illumina sequencing—generate Illumina-ready libraries with up to 384 combinations of indexes.

More Information

Alternative protocol for expanded input range between 10 ng–1 µg

To prepare libraries from inputs within 10 ng–1 µg, we recommend the following modifications to the number of cycles for PCR 1 (cDNA amplification; Section V.B of the user manual) and PCR 2 (library amplification; Section V.E of the user manual), below. For all other protocol steps, please follow the Smart-Seq Total RNA Pico Input (ZapR Mammalian) User Manual. 

Input PCR 1 cycles PCR 2 cycles*
>10 ng–100 ng 5 14
>100 ng–200 ng 5 12
>200 ng–1 μg 3 12

*PCR 2 cycles may be increased if library yields are low. See the user manual for more information. 

Applications

  • Robust NGS library construction that retains strand information
  • Use for RNA-seq on all Illumina platforms
  • Captures coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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  • Human ACE2 stable cell line
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  • Drug discovery
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  • Spatial omics
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  • Signature enzymes
  • High-throughput real-time PCR solutions
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  • Stem cells and stem cell-derived cells
  • mRNA and cDNA synthesis
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  • cDNA synthesis kits
  • Reverse transcriptases
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  • Purified total RNA and mRNA
  • PCR
  • Most popular polymerases
  • High-yield PCR
  • High-fidelity PCR
  • GC rich PCR
  • PCR master mixes
  • Cloning
  • In-Fusion seamless cloning
  • Competent cells
  • Ligation kits
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  • Nucleic acid purification
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  • Genomic DNA purification kits
  • DNA cleanup kits
  • RNA purification kits
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  • Viral transduction
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