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SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)

NOTE: SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) is a replacement for the SMART-Seq Stranded Kit with with some updates for improved performance as indicated below:

Improved efficiency of ZapR rRNA depletion reagents for better removal of unwanted reads
New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
For added flexibility, indexes are not included in the kit. A choice of indexing primers are sold separately (see Unique Dual Index kits)

Get more out of your single-cell and ultra-low input RNA-seq with SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian). This kit uses a random-priming approach to capture the whole transcriptome, enabling analysis of nonpolyadenylated transcripts at the single-cell level. The kit contains reagents for generating stranded sequencing libraries, including cDNA synthesis and amplification, ZapR-mediated ribosomal RNA depletion, and Illumina-compatible library preparation.

NOTE: SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) is a replacement for the SMART-Seq Stranded Kit with with some updates for improved performance as indicated below:

Improved efficiency of ZapR rRNA depletion reagents for better removal of unwanted reads
New kit sizes match those of Unique Dual Index kits (24 rxns, 96 rxns, 384 rxns)
For added flexibility, indexes are not included in the kit. A choice of indexing primers are sold separately (see Unique Dual Index kits)

This kit incorporates Takara Bio's SMART (Switching Mechanism at the 5' end of RNA Template) technology and includes refinements to the SMARTer method for stranded RNA-seq that simplify the library preparation workflow and improve sequencing performance. In addition, contrary to the SMART-Seq mRNA and SMART-Seq mRNA HT, reverse transcription is initiated using random priming instead of oligo(dT) priming, thus capturing the full transcriptome instead of only the polyadenylated fraction.

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. The integrated removal of cDNAs derived from rRNA-typically present in high abundance following cDNA synthesis from total RNA inputs-makes the workflow extremely sensitive, yielding data that is highly reproducible with low mapping to rRNA.

If you prefer an oligo(dT) priming approach, we recommend SMART-Seq mRNA LP (with UMIs), SMART-Seq mRNA LP, or SMART-Seq mRNA HT LP.

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Cat. #

Product

Size

License

Quantity

Details

634360

SMART-Seq® Total RNA Single Cell (ZapR™ Mammalian)

24 Rxns

USD $1500.00

License Statement

ID Number
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392	This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents.
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. The kit was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. Enough material is provided with this kit to perform up to 24 reactions.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

634361

SMART-Seq® Total RNA Single Cell (ZapR™ Mammalian)

96 Rxns

USD $4128.00

License Statement

ID Number
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392	This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents.
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

634362

SMART-Seq® Total RNA Single Cell (ZapR™ Mammalian)

4 x 96 Rxns

Inquire for Quotation

License Statement

ID Number
425	LIMITED USE LABEL LICENSE: RESEARCH USE ONLY Notice to Purchaser: This product is the subject to a license granted to Takara Bio USA, Inc. and its Affiliates from Caribou Biosciences, Inc., and this product is transferred to the end-user purchaser (“Purchaser”) subject to a “Limited Use Label License” conveying to the Purchaser a limited, nontransferable right to use the product, solely as provided to Purchaser, together with (i) progeny or derivatives of the product generated by the Purchaser (including but not limited to cells), and (ii) biological material extracted or derived from the product or its corresponding progeny or derivatives (including but not limited to cells) (collectively, the product, and (i) and (ii) are referred to as “Material”) only to perform internal research for the sole benefit of the Purchaser. The Purchaser cannot sell or otherwise transfer Material to a third party or otherwise use the Material for any Excluded Use. “Excluded Use” means any and all: (a) commercial activity including, but not limited to, any use in manufacturing (including but not limited to cell line development for purposes of bioproduction), product testing, or quality control; (b) preclinical or clinical testing or other activity directed toward the submission of data to the U.S. Food and Drug Administration, or any other regulatory agency in any country or jurisdiction where the active agent in such studies comprises the Material; (c) use to provide a service, information, or data to a third party with the sole exception of using the Material to conduct in vitro sample preparation, i.e., selectively depleting target cDNAs from a sample either by cleaving or selectively separating such target cDNAs from the sample through the use of the Materials; (d) use for human or animal therapeutic, diagnostic, or prophylactic purposes or as a product for therapeutics, diagnostics, or prophylaxis; (e) activity in an agricultural field trial or any activity directed toward the submission of data to the U.S. Department of Agriculture or any other agriculture regulatory agency; (f) high throughput screening drug discovery purposes (i.e., the screening of more than 10,000 experiments per day) as well as scale-up production activities for commercialization; (g) modification of human germline, including editing of human embryo genomes (with the sole exception of editing human embryonic stem (ES) cell lines for research purposes) or reproductive cells; (h) self-editing; and/or (i) stimulation of biased inheritance of a particular gene or trait or set of genes or traits (“gene drive”). It is the Purchaser’s responsibility to use the Material in accordance with all applicable laws and regulations. For information on obtaining additional rights, including commercial rights, please contact licensing@cariboubio.com or Caribou Biosciences, Inc., 2929 7th Street, Suite 105, Berkeley, CA 94710 USA, Attn: Licensing
392	This Product is protected by one or more patents from the family consisting of:US10941397, People's Republic of China Patent: ZL201480057094.4, US10781443, US10954510, DE602014069266.4, EP3058104, FR3058104, UK3058104, JP6602294, SE3058104, CA2923812, and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Information on relevant patents and licenses for this product may be found at: https://www.takarabio.com/patents.
395	This Product is protected by one or more patents from the family consisting of: US10150985, CA2939621, People's Republic of China Patent: ZL201480077658.0, US10988796, DE602014058059.9, EP3105325, FR3105325, UK3105325, JP6416939 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family. Additional information may be found at https://www.takarabio.com/patents.
450	This Product is sold under license from JumpCode Genomics, Inc., and is covered by one or more of the following US patents and foreign counterparts as well as pending US and foreign patent applications: 10,604,802; 11,708,606; 11,761,039; PCT/US2015/014242; CA2938669; EP 20192599.7; HK402021031164.3.

Notice to purchaser

Components

Required Products

Cat. #	Product	Size	Price
634752	Unique Dual Index Kit (1–96)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634752: Unique Dual Index Kit (1-96)
634753	Unique Dual Index Kit (97–192)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634753: Unique Dual Index Kit (97-192)
634754	Unique Dual Index Kit (193–288)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634754: Unique Dual Index Kit (193-288)
634755	Unique Dual Index Kit (289–384)	96 Rxns	USD $599.00
The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634755: Unique Dual Index Kit (289-384)
634756	Unique Dual Index Kit (1–24)	24 Rxns	USD $222.00
The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits. Documents Components Image Data Back 634756: Unique Dual Index Kit (1-24)

Overview

Simple workflow to generate stranded Illumina sequencing-ready libraries in 7 hours
Appropriate for analysis of 1–1,000 intact mammalian cells or 10 pg–10 ng of low- or high-quality mammalian total RNA
Reproducible, accurate detection of full-length coding and noncoding transcripts from single cells or total RNA
Comparable sensitivity to our gold-standard SMART-Seq mRNA.

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) workflow

Schematic of workflow for SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)

More Information

Applications

Generation of Illumina sequencing-ready libraries in 7 hours from small quantities of mammalian cells (1–1,000 cells) or total RNA (10 pg–10 ng)
Whole transcriptome sequencing with strand-of-origin information

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian)

Notice to purchaser

Efficient depletion of rRNA-derived cDNAs across a wide input range

Superior gene and transcript detection across a wide input range

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Notice to purchaser

Efficient depletion of rRNA-derived cDNAs across a wide input range

Superior gene and transcript detection across a wide input range

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Notice to purchaser

Efficient depletion of rRNA-derived cDNAs across a wide input range

Superior gene and transcript detection across a wide input range

634752: Unique Dual Index Kit (1-96)

634753: Unique Dual Index Kit (97-192)

634754: Unique Dual Index Kit (193-288)

634755: Unique Dual Index Kit (289-384)

634756: Unique Dual Index Kit (1-24)

Overview

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) workflow

More Information

Applications

Additional product information