Cogent NGS Analysis Pipeline

Version 2.0

Now compatible with our popular bulk and plate-based RNA-seq kits!

Overview

Cogent NGS Analysis Pipeline (also referred to as CogentAP) is bioinformatic software for analyzing RNA-seq data, including gene or transcript expression counting, gene fusion detection, and immune profiling analysis. Please refer to the table at the bioinformatics resource portal for the full list of validated Takara Bio reagent kits supported for use with the pipeline. CogentAP inputs raw sequencing data and outputs an HTML report and other files, such as a gene matrix, to continue further analysis. Output also includes R data objects, which may be used as input for Cogent NGS Discovery Software.

After installing, the software consists of the following features:

CogentAP GUI launcher
Command-line interface program
Mini-dataset files (WellList.txt and FASTQ files)—used to test the CogentAP functionality
Sample output reports and stats—used to compare to the analysis results of the mini-dataset test

Sign up to get the Cogent NGS Analysis Pipeline

If you are interested in using CogentAP, please review the End User License Agreement (EULA) before populating the required fields of the form.

After checking the box to indicate acceptance of the EULA and submitting the completed form, your browser will be directed to a page where you can download the software. You will be emailed a code to activate it. Installation instructions can be found in Cogent NGS Analysis Pipeline v2.0 User Manual and Quick Start Guide.

Supported operating systems

CogentAP can be installed on the following versions of Linux:

CentOS 6.9 & 6.10
RedHat 7.5
Ubuntu 17

Hardware requirements

For analyzing Illumina NextSeq® High-Output data:

24-core CPU
64 GB+ RAM
500 GB+ available hard drive space

Analyzing MiniSeq™ or MiSeq® data may be possible on servers with less computational power than the specifications above. Analysis of HiSeq® or NovaSeq™ data would require better hardware than is listed.

Additional third-party software dependencies*

Conda (or Miniconda3)
bcl2fastq conversion software

*Not included with the software; must be downloaded and installed separately.

Supported applications

See a list of supported applications.

Additional product information

For use in conjunction with workflows on the ICELL8 cx Single-Cell System, ICELL8 Single-Cell System, or select Takara Bio UMI kits. More information is available in the Cogent NGS Analysis Pipeline v2.0 User Manual and Quick Start Guide.

Cogent NGS Analysis Pipeline version history

Version number	Release date	Notes
CogentAP 2.0	2022-08-17	New plate-based kit data input option: SMART-Seq mRNA LP (with UMIs), SMART-Seq mRNA, and SMART-Seq mRNA LP (refer to the bioinformatics resources portal for a full list of supported kits)
CogentAP 1.5.1	2022-03-25	New plate-based kit data input options Adds Nextera®- and TruSeq®-based adapter sequences for trimming New default gene fusion analysis filtering level to improve identification of true fusions New variable to customize filtering levels for gene fusion analysis (CLI-only) Minor bug fixes Documentation: User Manual Quick Start Guide
CogentAP 1.5	2021-12-03	Prerequisites: Requires Miniconda3 version 4.10.2 or higher Improved support for full-length transcriptomics, with additional options for transcript, gene fusion, and immune profiling analysis New kit data input options: SMART-Seq Pro Application Kit - 2 Chip Documentation: User Manual Quick Start Guide
CogentAP 1.0	2020-09-11	Prerequisites: Requires Miniconda3 version 4.8.2 or higher Requires bcl2fastq New kit data input options: ICELL8 Single-Cell System 3' DE for UMI workflow, SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (includes UMIs) Documentation: User Manual Quick Start Guide EULA
mappa Analyzer 1.0	2019-06-07	Prerequisites: Requires Miniconda3 version 4.5.0 or later Kit data input options: ICELL8 system sequencing results of full-length transcriptome or single-cell differential expression (3' DE or 5' DE) workflows, no UMI support Documentation: User Guide EULA

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