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  • Single-cell full-length transcriptome analysis
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  • Single-cell differential expression analysis
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Home › Products › Next-generation sequencing › Single-cell NGS automation › ICELL8 cx applications › Single-cell full-length transcriptome analysis

Single-cell NGS automation

  • ICELL8 cx applications
    • Single-cell full-length transcriptome analysis
    • Human TCR profiling and 5' DE
    • Single-cell differential expression analysis
  • ICELL8 applications
    • Single-cell full-length transcriptome analysis
    • Human TCR profiling and 5' DE
    • Single-cell differential expression analysis
    • Single-cell ATAC-seq
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Single-cell full-length transcriptome analysis on the ICELL8 cx system powered by SMART-Seq technology

NGS logo

Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.

Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.

Cells are dispensed into a 5,184-nanowell, blank ICELL8 350v Chip (Cat. # 640019) using the ICELL8 cx Single-Cell System (Cat. # 640188). After identifying single-cell-bearing wells using ICELL8 cx CellSelect Software, reagents are dispensed only to wells chosen for further processing. After a series of dispense and amplification steps, unbiased, full-length cDNA is produced and tagmented by Illumina Bead-Linked Transposome (BLT). The scalable workflow incorporates Illumina-specific adapter sequences during cDNA amplification to generate indexed libraries that are ready for sequencing on Illumina platforms. Two unique index plates are provided in each kit to allow for two chip libraries to be processed together in a single sequencer run. After mRNA sequencing is completed, data can be imported directly into Cogent NGS software tools for bioinformatics analysis and interpretation of results.

NOTE: Past versions of SMART-Seq kits for the ICELL8 cx system are still available for sale. Contact a customer service representative if interested in Cat. # 640222, 640223, or 640224.

If interested in plate-seq applications, please refer to the RNA-seq product page.

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Cat. # Product Size License Quantity Details
640257 SMART-Seq® Pro Application Kit - 2 Chip 2 Chips USD $4950.00

License Statement

ID Number  
275 SMART-Seq2 Technology. This product is sold under exclusive license from Ludwig Institute of Cancer Research, Ltd. and is covered by US Patent No. 10266894, Japanese Patent No. 6336080, and European Patent No. 3036336, and pending U.S. patent application and/or pending claims of foreign counterparts. For license information, please contact a Takara Bio USA, Inc. licensing representative by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.

SMART-Seq Pro Application kit contains the necessary chips, reagents, and consumables required to run two complete experiments of single-cell, full-length transcriptome analysis on the ICELL8 cx Single-Cell System (Cat. No. 640188).

The protocols for performing SMART-Seq chemistry on ICELL8 systems leverage SMART technology for full-length cDNA synthesis and Nextera® technology for Illumina library preparation with dual 72 x 72 indexes (Nextera Tagmentation bead-linked transposome not included). The protocols generate indexed libraries that are ready for sequencing on Illumina platforms. Provided index plates allow for two chip libraries to be processed simultaneously in a single sequencer run.

ICELL8/MSND 384-Well Source Plate and Seals (Cat. Nos. 640192, 640018, or 640037) need to be purchased separately. A total of four plates and seals are required to process each individual chip.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components You May Also Like Image Data

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Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

Comparison of genes detected from PBMCs using SMART-Seq Pro or Smart-seq2 (SS2) workflows.

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Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium.

Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium.

Comparison of isoform data generated using the SMART-Seq Pro kit or 10x Chromium. The SMART-Seq Pro method using the ICELL8 cx system can identify junction reads across exons 3, 4, 5, 6, and 7 and shows distinct profiles for PTPRC-201 and PTPRC-209 isoforms. Data generated from 10x Chromium end-counting methods could not identify junctions in these same exon regions, with peaks representing noise from misaligned reads.

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640257: SMART-Seq Pro Application Kit - 2 Chip

640257: SMART-Seq Pro Application Kit - 2 Chip
640192 ICELL8® 384-Well Source Plate and Seal Each USD $175.00

The ICELL8 384-Well Source Plate and Seal set is a component of the ICELL8 cx Single-Cell System. It includes NUNC plates and seals for covering the plates, which are utilized for sample and reagent dispense into SmartChip plates for related NGS applications.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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640192: ICELL8 384-Well Source Plate and Seal

640192: ICELL8 384-Well Source Plate and Seal
640018 MSND 384-Well Source Plate and Seals (430-000025) 20/Pack USD $450.00

20 Pack of MSND 384-Well Source Plate and Seals. Formerly 430-000025.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

Back

640018: MSND 384-Well Source Plate and Seals (430-000025)

640018: MSND 384-Well Source Plate and Seals (430-000025)
640037 MSND 384-well Source Plate and Seals (430-000258) 120/Pack USD $1665.00

120 pack of MSND 384-well Source Plate and Seals. Formerly 430-000258.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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640037: MSND 384-well Source Plate and Seals (430-000258)

640037: MSND 384-well Source Plate and Seals (430-000258)

SSsc Pro Application Kit User Manual SMART-Seq Pro kit user manual
Technical note SMART-Seq Pro kit technical note
SS Pro webinar Webinar: complete biomarker discovery

Overview

  • End-to-end solution—includes library preparation and data analysis tools
  • Full-length mRNA coverage—capture critical biomarkers like gene fusions and splice variants deeper within the transcriptome
  • High-throughput single-cell analysis—generate NGS libraries from >1,000 single cells in a single nanowell chip experiment
  • Sequencing efficiency—incorporate unique index primer sets to process two chip libraries at once for up to 3,000 cells of data per sequencing run, on all Illumina platforms
  • User-friendly bioinformatics—align, map, and visualize findings with Cogent NGS Analysis Pipeline and Cogent NGS Discovery Software
  • Sample flexibility—analyze cells from up to eight different samples in a single experiment
  • Reduced sample handling—minimize variability and dispense only to isolated single cells of interest with automated cell identification and selection prior to sequencing-ready library construction

More Information

Applications

  • High-throughput, full-length scRNA-seq library preparation for Illumina platforms

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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Overview of the ICELL8 cx Single-Cell System

Learn how ICELL8 technology allows flexibility, confidence, and control in your single-cell analysis.

Learn more


Single-cell identification with CellSelect Software

See how the ICELL8 platform's Imaging System and CellSelect Software allow for high-fidelity identification of individual cells.

Learn more

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