Single-cell full-length transcriptome analysis on the ICELL8 cx system powered by SMART-Seq technology
Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.
Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.
Cells are dispensed into a 5,184-nanowell, blank ICELL8 350v Chip (Cat. # 640019) using the ICELL8 cx Single-Cell System (Cat. # 640188). After identifying single-cell-bearing wells using ICELL8 cx CellSelect Software, reagents are dispensed only to wells chosen for further processing. After a series of dispense and amplification steps, unbiased, full-length cDNA is produced and tagmented by Illumina Bead-Linked Transposome (BLT). The scalable workflow incorporates Illumina-specific adapter sequences during cDNA amplification to generate indexed libraries that are ready for sequencing on Illumina platforms. Two unique index plates are provided in each kit to allow for two chip libraries to be processed together in a single sequencer run. After mRNA sequencing is completed, data can be imported directly into Cogent NGS software tools for bioinformatics analysis and interpretation of results.
NOTE: Past versions of SMART-Seq kits for the ICELL8 cx system are still available for sale. Contact a customer service representative if interested in Cat. # 640222, 640223, or 640224.
If interested in plate-seq applications, please refer to the RNA-seq product page.
Overview
- End-to-end solution—includes library preparation and data analysis tools
- Full-length mRNA coverage—capture critical biomarkers like gene fusions and splice variants deeper within the transcriptome
- High-throughput single-cell analysis—generate NGS libraries from >1,000 single cells in a single nanowell chip experiment
- Sequencing efficiency—incorporate unique index primer sets to process two chip libraries at once for up to 3,000 cells of data per sequencing run, on all Illumina platforms
- User-friendly bioinformatics—align, map, and visualize findings with Cogent NGS Analysis Pipeline and Cogent NGS Discovery Software
- Sample flexibility—analyze cells from up to eight different samples in a single experiment
- Reduced sample handling—minimize variability and dispense only to isolated single cells of interest with automated cell identification and selection prior to sequencing-ready library construction
More Information
Applications
- High-throughput, full-length scRNA-seq library preparation for Illumina platforms
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Overview of the ICELL8 cx Single-Cell System
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