Single-cell full-length transcriptome analysis on the ICELL8 cx system powered by SMART-Seq technology
Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.
Full-length sequence coverage enables differential expression analysis across the entire transcriptome and detection of biomarkers like gene fusions and splice variants. Empowering these analyses is SMART-Seq Pro technology—our highest sensitivity yet for automated single-cell RNA-seq. Combining SMART-Seq chemistry with the ICELL8 cx system and integrated bioinformatic tools, the kit’s end-to-end workflow enables efficient, cost-effective analysis of 1,000–2,000 isolated cells from a single chip.
Cells are dispensed into a 5,184-nanowell, blank ICELL8 350v Chip (Cat. # 640019) using the ICELL8 cx Single-Cell System (Cat. # 640188). After identifying single-cell-bearing wells using ICELL8 cx CellSelect Software, reagents are dispensed only to wells chosen for further processing. After a series of dispense and amplification steps, unbiased, full-length cDNA is produced and tagmented by Illumina Bead-Linked Transposome (BLT). The scalable workflow incorporates Illumina-specific adapter sequences during cDNA amplification to generate indexed libraries that are ready for sequencing on Illumina platforms. Two unique index plates are provided in each kit to allow for two chip libraries to be processed together in a single sequencer run. After mRNA sequencing is completed, data can be imported directly into Cogent NGS software tools for bioinformatics analysis and interpretation of results.
NOTE: Past versions of SMART-Seq kits for the ICELL8 cx system are still available for sale. Contact a customer service representative if interested in Cat. # 640222, 640223, or 640224.
If interested in plate-seq applications, please refer to the RNA-seq product page.
Overview
- End-to-end solution—includes library preparation and data analysis tools
- Full-length mRNA coverage—capture critical biomarkers like gene fusions and splice variants deeper within the transcriptome
- High-throughput single-cell analysis—generate NGS libraries from >1,000 single cells in a single nanowell chip experiment
- Sequencing efficiency—incorporate unique index primer sets to process two chip libraries at once for up to 3,000 cells of data per sequencing run, on all Illumina platforms
- User-friendly bioinformatics—align, map, and visualize findings with Cogent NGS Analysis Pipeline and Cogent NGS Discovery Software
- Sample flexibility—analyze cells from up to eight different samples in a single experiment
- Reduced sample handling—minimize variability and dispense only to isolated single cells of interest with automated cell identification and selection prior to sequencing-ready library construction
More Information
Applications
- High-throughput, full-length scRNA-seq library preparation for Illumina platforms
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
New related product names
Several products associated with the ICELL8 cx Single-Cell System are in the process of being renamed. The tables below document the old and new names as a reference for you on the website and in our documentation while the changes are being implented.
The Catalog Number for each product remains unchanged, so you can continue ordering without issue.
Experiment consumables
| Old product name | Cat. # | New product name |
|---|---|---|
| MSND 384-Well Source Plate and Seals | 640018 640037 |
Nanodispenser 384-Well Source Plate and Seal |
| ICELL8 350v Chip | 640019 | Single-Cell 350v Chip |
| Intermediate Film | 640031 | Nanodispenser Chip Intermediate Film |
| ICELL8 cx Blank Chip - 250 nl FB | 640193 | Single-Cell 250f Chip |
| SmartChip Intermediate Film | 640197 | Nanodispenser Chip Intermediate Film |
| ICELL8 Loading Kit – B | 640206 | Single-Cell Loading Kit |
| ICELL8 Collection Kit – L | 640212 | Single-Cell Collection Kit |
| Optical Imaging Film | – | Chip Freezing Film |
| Collection Fixture – L | – | Single-Cell Collection Fixture |
Accessories of the Shasta Single Cell System (Cat # 640282) and the ICELL8 cx Single-Cell System (Cat # 640188)
| Old product name | Cat. # | New product name |
|---|---|---|
| ICELL8 cx Thermal Cycler | 640002 | Single-Cell Thermal Cycler |
| ICELL8 Chip Holder | 640008 | Nanodispenser Chip Holder |
| ICELL8 cx Accessory Kit | 640194 | Single-Cell Accessory Kit |
| ICELL8 Extraction Fixture – L | 640217 | Single-Cell Extraction Fixture |
| ICELL8 cx Centrifuge Balance Plate | 384-Well Plate Balance | |
| Plate Seal Applicator | 384-Well Plate Seal Applicator | |
| Chip blotter | Nanodispenser Chip Blotter | |
| ICELL8 cx Centrifuge Chip Spinner | – | Nanodispenser Chip Centrifuge Spinner |
| ICELL8 cx Cold Block | – | Nanodispenser Chip Cold Block |
Overview of the ICELL8 cx Single-Cell System
Learn how ICELL8 technology allows flexibility, confidence, and control in your single-cell analysis.
Single-cell identification with CellSelect Software
See how the ICELL8 platform's Imaging System and CellSelect Software allow for high-fidelity identification of individual cells.
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