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  • Embgenix ESM Screen Kit
  • Embgenix PGT-A Kit (RUO)
Bioinformatics software Embgenix Analysis Software
Embgenix PGT-A Kit (CE-IVD) Product Page Embgenix PGT-A Kit (CE-IVD) product page
Home › Products › Next-generation sequencing › Reproductive health › Embgenix PGT-A Kit (RUO)

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Bioinformatics software Embgenix Analysis Software
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Embgenix PGT-A Kit (RUO)

Reproductive health

The Embgenix preimplantation genetic testing for aneuploidy (PGT-A) is a research use only (RUO) kit designed to identify embryos with the correct number of chromosomes and thus have the greatest potential for a successful pregnancy during in vitro fertilization (IVF). The kit utilizes a next-generation sequencing method compatible with all Illumina sequencers and can be adopted for medium- to high-throughput genetic testing labs and IVF clinics offering the PGT-A test.

Interested in a CE-marked version of this product? Access the Embgenix PGT-A Kit (CE-IVD) product page.

The Embgenix preimplantation genetic testing for aneuploidy (PGT-A) is a research use only (RUO) kit designed to identify embryos with the correct number of chromosomes and thus have the greatest potential for a successful pregnancy during in-vitro fertilization (IVF). The kit was developed following extensive analytical and clinical validation studies and accurately identifies whole chromosome aneuploidies on all 24 chromosomes, including Trisomy 21. In addition, you can identify segmental aneuploidy and mosaicism (with limited thresholds). The kit utilizes a next-generation sequencing method compatible with all Illumina sequencers and can be adopted for medium- to high-throughput genetic testing labs and IVF clinics offering the PGT-A test.

The kit utilizes the gold-standard PicoPLEX whole genome amplification (WGA) and patented library-prep technology with unique dual indexes to streamline the generation of sequencing libraries. PicoPLEX quasi-random priming technology is widely accepted for accurate and reproducible detection of aneuploidies and copy number variants (CNVs) from single cells and low-input DNA. The simple protocol dramatically improves time to results and reduces handling errors and background. The library-prep workflow (fragmentation, repair, ligation, amplification, and indexing) takes place in a single tube in about two hours. No intermediate purification steps or sample transfers are necessary, minimizing sample mix-up and loss.

After sequencing, use the Embgenix Analysis Software (RUO), our specialized cloud-based bioinformatics pipeline, for data analysis and reporting. The algorithm is powered to compute calculated copy numbers (CCNs) using bin counts against an internally configured reference for accurate determination of chromosomal CNVs. The software allows the option for both automated and/or manual calls for whole chromosome, segmental, and mosaic aneuploidies, and data can be conveniently visualized in a chart or an idiogram plot. The software also supports downloadable PDF reports, which can be customized for individual laboratory needs.

Interested in a CE-marked version of this product? Access the Embgenix PGT-A Kit (CE-IVD) product page.

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Cat. # Product Size License Quantity Details
634760 Embgenix™ PGT-A Kit (RUO) 96 Rxns Inquire for Quotation

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
328 This product is protected by U.S. Patents 8,206,913 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337, 11,072,823 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

This preimplantation genetic testing kit is based on PicoPLEX WGA technology and yields sequencing libraries from 3–10 TE biopsy cells or genomic DNA equivalents, providing reliable results for the detection of aneuploidies and CNVs. This product contains reagents for 96 reactions and unique dual indices for multiplexing.

The kit features a streamlined workflow with three steps. First, an effective lysis procedure is applied to ensure robust cell lysis, making the DNA template readily accessible for WGA. The second step is the WGA reaction which uses the DNA template from step one for preamplification and amplification using a proprietary quasi-linear amplification approach. This approach reduces false signals in the final libraries by ensuring that low-level errors introduced during PCR are not amplified. In the third step, a streamlined workflow generates Illumina sequencing libraries using our proprietary SMART technology and unique dual indices.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Embgenix PGT-A workflow

Embgenix PGT-A workflow

Protocol overview. Starting from gDNA, with automation, in six to eight hours any lab can generate libraries in 96-well format ready for sequencing on an Illumina platform.

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Embgenix plot showing genomic DNA sample containing trisomy of Chr18

Embgenix plot showing genomic DNA sample containing trisomy of Chr18

Emgenix PGT-A detection of Chr 18 trisomy. Embgenix PGT-A correctly predicts a full chromosome aneuploidy in Chr18, linked to Edwards Syndrome, in a gDNA sample.

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Segmental aneuploidy detection using the Embgenix PGT-A Kit

Segmental aneuploidy detection using the Embgenix PGT-A Kit

Segmental aneuploidy detection using the Embgenix PGT-A Kit. (Top) Embgenix PGT-A correctly predicts two segmental aneuploidies in ChrX, linked to Turner Syndrome, in Coriell gDNA sample NA13019. (Bottom) Embgenix PGT-A correctly predicts two segmental aneuploidies in Chr8, linked to hydrocephalus, in Coriell gDNA sample NA14485.

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Aneuploidy assessment conducted by clinical laboratory C demonstrates concordance between results from Embgenix PGT-A analysis and results from established PGT-A methods

Aneuploidy assessment conducted by clinical laboratory C demonstrates concordance between results from Embgenix PGT-A analysis and results from established PGT-A methods
Representative example data from Embryo #9 is shown. Aneuploidy assessment of two TE biopsies and one ICM biopsy were performed using Embgenix PGT-A and an established method, VeriSeq PGS Kit with BlueFuse Multi Software. All indicated monosomy of Chr5. Top row: Data from a TE sample assessed with VeriSeq PGS Kit with BlueFuse Multi Software. Middle row: Data from a ICM sample assessed with Embgenix PGT-A and Embgenix Analysis Software. Bottom row: Data from a TE sample assessed with Embgenix PGT-A and Embgenix Analysis Software.

Overview

  • Complete PGT-A workflow, including WGA, library-prep, and aneuploidy analysis
  • PGT-A validation using trophoectoderm embryo (TE) biopsy samples
  • Single-step WGA with reduced hands-on-time and library prep with enzymatic fragmentation
  • Unparalleled sensitivity and reproducibility for CNV detection
  • Scalable workflow to incorporate multiple throughputs (24 to 96 samples)
  • Compatible with Miseq® and NextSeq® 500/550 Illumina sequencers
  • Increased sequencing power with unique dual indexes included to allow pooling of multiple samples and confident sequencing

More Information

Applications

  • Aneuploidy and CNV detection in gDNA and embryo samples
  • Preimplantation genetic testing for aneuploidies (detection of Trisomy 21 is for research use only)

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.


Bioinformatics solution for this kit

Embgenix Analysis Software (RUO)

Embgenix Analysis Software is a cloud-based bioinformatics software for analysis of sequencing data producing using the Embgenix PGT-A Kit for the Embgenix ESM Screen Kit

Embgenix Analysis Software


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Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.

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