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Embgenix PGT-A Kit (RUO)

Reproductive health

The Embgenix PGT-A Kit (RUO) and accompanying analysis software provide a complete solution for next-generation sequencing (NGS)-based detection of chromosomal copy number variations (CNVs) from embryo biopsy samples. The kit enables accurate identification of whole chromosome, segmental, and mosaic aneuploidies, and yields Illumina-compatible sequencing libraries in a scalable format to support medium- to high-throughput applications.

The Embgenix PGT-A Kit (RUO) and accompanying analysis software provide a complete solution for next-generation sequencing (NGS)-based detection of chromosomal copy number variations (CNVs) from embryo biopsy samples. The kit enables accurate identification of whole chromosome, segmental, and mosaic aneuploidies, and yields Illumina-compatible sequencing libraries in a scalable format to support medium- to high-throughput applications.

The kit employs PicoPLEX technology for highly reproducible whole genome amplification (WGA) and a streamlined library prep methodology that minimizes hands-on time and the likelihood of sample processing errors. PicoPLEX WGA is a widely applied technology for CNV detection from single cells and low-input DNA, both in the context of reproductive health research and beyond. The entire library preparation workflow can be completed in a single tube in about two hours, yielding NGS libraries that are broadly compatible with Illumina sequencing platforms and allow for multiplexing of up to 96 samples per sequencing run.

After sequencing, cloud-based Embgenix Analysis Software (RUO) is used for data analysis and reporting. The software’s algorithm is designed to compute calculated copy numbers (CCNs) using bin counts against an internally configured reference for accurate determination of chromosomal CNVs. The software supports both automated and manual calling of whole chromosome, segmental, and mosaic CNVs, and data can be conveniently visualized in a chart or an idiogram plot. The software also provides a variety of data output options, including bulk download of results and generation of customizable PDF reports.

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Cat. # Product Size Price License Quantity Details
634760 Embgenix™ PGT-A Kit (RUO) 96 Rxns Inquire for Quotation

License Statement

ID Number  
M54 This product is covered by the claims of U.S. Patent Nos. 7,704,713 and its foreign counterparts. 
328 This Product is protected by one or more patents from the family consisting of: CH1604040, CH2374900, US8206913, US10837049, US11661628, US11492663, DE602004029560.4, DE602004049599.9, DK1604040, DK2374900, EP1604040, EP2374900, FR1604040, FR2374900, UK1604040, UK2374900, HK1089485, IE1604040, IE2374900, JP4773338, NL1604040, NL2374900, SE1604040, SE2374900 and any corresponding patents, divisionals, continuations, patent applications and foreign filings sharing common priority with the same family.
326 This product is protected by U.S. Patents 7,803,550; 8,399,199; 8,728,737, 9,598,727, 10,196,686, 10,208,337, 11,072,823 and corresponding foreign patents. Additional patents are pending. For further license information, please contact a Takara Bio USA licensing representative by email at licensing@takarabio.com.
*

The Embgenix PGT-A Kit (RUO) provides a complete solution for NGS-based detection of copy number variations (CNVs) from 3–10 TE biopsy cells or genomic DNA equivalents. The kit employs PicoPLEX whole genome amplification (WGA) technology and a streamlined library prep methodology that can be completed in a single tube in about two hours, minimizing hands-on time and the likelihood of sample processing errors. Sequencing libraries produced with the kit are broadly compatible with Illumina platforms and provided unique dual indexes allow for multiplexing of up to 96 samples in a single sequencing run. Resulting data is analyzed using intuitive, cloud-based Embgenix Analysis Software (RUO), which employs a proprietary algorithm for CNV detection and provides an array of customizable features and reporting options.

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Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

Documents Components Image Data

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Embgenix PGT-A workflow

Embgenix PGT-A workflow

Protocol overview. Starting from gDNA, with automation, in six to eight hours any lab can generate libraries in 96-well format ready for sequencing on an Illumina platform.

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Embgenix plot showing genomic DNA sample containing trisomy of Chr18

Embgenix plot showing genomic DNA sample containing trisomy of Chr18

Emgenix PGT-A detection of Chr 18 trisomy. Embgenix PGT-A correctly predicts a full chromosome aneuploidy in Chr18, linked to Edwards Syndrome, in a gDNA sample.

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Segmental aneuploidy detection using the Embgenix PGT-A Kit

Segmental aneuploidy detection using the Embgenix PGT-A Kit

Segmental aneuploidy detection using the Embgenix PGT-A Kit. (Top) Embgenix PGT-A correctly predicts two segmental aneuploidies in ChrX, linked to Turner Syndrome, in Coriell gDNA sample NA13019. (Bottom) Embgenix PGT-A correctly predicts two segmental aneuploidies in Chr8, linked to hydrocephalus, in Coriell gDNA sample NA14485.

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Aneuploidy assessment conducted by clinical laboratory C demonstrates concordance between results from Embgenix PGT-A analysis and results from established PGT-A methods

Aneuploidy assessment conducted by clinical laboratory C demonstrates concordance between results from Embgenix PGT-A analysis and results from established PGT-A methods
Representative example data from Embryo #9 is shown. Aneuploidy assessment of two TE biopsies and one ICM biopsy were performed using Embgenix PGT-A and an established method, VeriSeq PGS Kit with BlueFuse Multi Software. All indicated monosomy of Chr5. Top row: Data from a TE sample assessed with VeriSeq PGS Kit with BlueFuse Multi Software. Middle row: Data from a ICM sample assessed with Embgenix PGT-A and Embgenix Analysis Software. Bottom row: Data from a TE sample assessed with Embgenix PGT-A and Embgenix Analysis Software.

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CNV classifications applied by Embgenix Analysis Software

CNV classifications applied by Embgenix Analysis Software

Copy number variant (CNV) classifications applied by Embgenix Analysis Software. Classification categories are listed from highest to lowest priority. Samples interpreted to have multiple different CNVs are classified according to the CNV with the highest priority.

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Study with 22 samples, showing accurate reporting of segmental aneuploidies including size prediction

Study with 22 samples, showing accurate reporting of segmental aneuploidies including size prediction

Study with 22 samples, showing accurate reporting of segmental aneuploidies including size prediction. A panel of genomic DNA samples of known CNV status from the Coriell Institute was analyzed to demonstrate the capacity of the Embgenix PGT-A assay to identify segmental aneuploidies of varying size at different locations across the genome. Each segmental aneuploidy was successfully identified (including aneuploidies falling below the 10-Mb size threshold for the assay), with the predicted size of each aneuploidy closely matching the true, established size. As indicated, the underestimated size of the CNV on chromosome 15 in the sample NA20556 was likely impacted by the low mappability of its genomic context.

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Comparison of mosaic detection capability between Embgenix PGT-A and Reproseq

Comparison of mosaic detection capability between Embgenix PGT-A and Reproseq

Comparison of mosaic detection capability between Embgenix PGT-A and Reproseq. Sensitivity of mosaic detection is compared side-by-side for Embgenix PGT-A and Reproseq, using artificial segmental mosaicisms generated by mixing two Coriell gDNA samples (NA05966 and NA10925) containing segmental aneuploidies in different genomic locations. The two Coriell gDNA samples were mixed at the five ratios indicated. CNVs reported by each method are marked as true positives (blue arrows) or false negatives (red squares), with no false positives reported by either method.

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634760: Embgenix PGT-A Kit (RUO)

634760: Embgenix PGT-A Kit (RUO)

*You must be logged in to a Purchasing Account in order to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@takarabio.com.

Overview

  • Complete solution for NGS-based CNV detection, including WGA, library preparation, and bioinformatic analysis
  • Reliable detection of CNVs from trophectoderm (TE) biopsy samples, including whole-chromosome and segmental gains/losses ≥10 Mb and mosaicisms ≥30%
  • Powered by industry-leading PicoPLEX WGA technology, providing unparalleled reproducibility and coverage uniformity
  • Scalable library prep workflow requires about two hours of hands-on time and allows for multiplexing of up to 96 samples
  • Broad compatibility with Illumina sequencing platforms; validated protocols provided for MiSeq® and NextSeq® 500/550
  • Intuitive, cloud-based analysis software provided for streamlined processing of sequencing data and reporting of results

More Information

Applications

  • Detection of CNVs in gDNA and embryo samples
  • Reproductive health research

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.


Bioinformatics solution for this kit

Embgenix Analysis Software for ESM Screen Kit

Embgenix Analysis Software for ESM Screen Kit is a cloud-based bioinformatics software for analysis of sequencing data producing using the Embgenix ESM Screen Kit.

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