Embgenix PGT-A Kit (RUO)
The Embgenix preimplantation genetic testing for aneuploidy (PGT-A) is a research use only (RUO) kit designed to identify embryos with the correct number of chromosomes and thus have the greatest potential for a successful pregnancy during in vitro fertilization (IVF). The kit utilizes a next-generation sequencing method compatible with all Illumina sequencers and can be adopted for medium- to high-throughput genetic testing labs and IVF clinics offering the PGT-A test.
Interested in a CE-marked version of this product? Access the Embgenix PGT-A Kit (CE-IVD) product page.
The Embgenix preimplantation genetic testing for aneuploidy (PGT-A) is a research use only (RUO) kit designed to identify embryos with the correct number of chromosomes and thus have the greatest potential for a successful pregnancy during in-vitro fertilization (IVF). The kit was developed following extensive analytical and clinical validation studies and accurately identifies whole chromosome aneuploidies on all 24 chromosomes, including Trisomy 21. In addition, you can identify segmental aneuploidy and mosaicism (with limited thresholds). The kit utilizes a next-generation sequencing method compatible with all Illumina sequencers and can be adopted for medium- to high-throughput genetic testing labs and IVF clinics offering the PGT-A test.
The kit utilizes the gold-standard PicoPLEX whole genome amplification (WGA) and patented library-prep technology with unique dual indexes to streamline the generation of sequencing libraries. PicoPLEX quasi-random priming technology is widely accepted for accurate and reproducible detection of aneuploidies and copy number variants (CNVs) from single cells and low-input DNA. The simple protocol dramatically improves time to results and reduces handling errors and background. The library-prep workflow (fragmentation, repair, ligation, amplification, and indexing) takes place in a single tube in about two hours. No intermediate purification steps or sample transfers are necessary, minimizing sample mix-up and loss.
After sequencing, use the Embgenix Analysis Software (RUO), our specialized cloud-based bioinformatics pipeline, for data analysis and reporting. The algorithm is powered to compute calculated copy numbers (CCNs) using bin counts against an internally configured reference for accurate determination of chromosomal CNVs. The software allows the option for both automated and/or manual calls for whole chromosome, segmental, and mosaic aneuploidies, and data can be conveniently visualized in a chart or an idiogram plot. The software also supports downloadable PDF reports, which can be customized for individual laboratory needs.
Interested in a CE-marked version of this product? Access the Embgenix PGT-A Kit (CE-IVD) product page.
Overview
- Complete PGT-A workflow, including WGA, library-prep, and aneuploidy analysis
- PGT-A validation using trophoectoderm embryo (TE) biopsy samples
- Single-step WGA with reduced hands-on-time and library prep with enzymatic fragmentation
- Unparalleled sensitivity and reproducibility for CNV detection
- Scalable workflow to incorporate multiple throughputs (24 to 96 samples)
- Compatible with Miseq® and NextSeq® 500/550 Illumina sequencers
- Increased sequencing power with unique dual indexes included to allow pooling of multiple samples and confident sequencing
More Information
Applications
- Aneuploidy and CNV detection in gDNA and embryo samples
- Preimplantation genetic testing for aneuploidies (detection of Trisomy 21 is for research use only)
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab.
Bioinformatics solution for this kit
Embgenix Analysis Software (RUO)
Embgenix Analysis Software is a cloud-based bioinformatics software for analysis of sequencing data producing using the Embgenix PGT-A Kit for the Embgenix ESM Screen Kit
Inquire for Quotation
Customers in Europe interested in this product should send email to ivd_eu@takarabio.com. Other customers should contact your local Takara Bio affiliate or distributor for more information about pricing or purchasing.
Find your local affiliate or distributorTakara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
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