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  • Cogent NGS Analysis Pipeline
  • Cogent NGS Discovery Software
  • Cogent NGS Immune Profiler
  • Cogent NGS Immune Viewer
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Immune Profiler user manual Cogent NGS Immune Profiler User Manual
Quick Start document CogentIP and Immune Viewer Quick Start Guide
Immune Profiler troubleshooting FAQ Immune Profiler troubleshooting guide
hBCRv2 product page SMART-Seq Human BCR (with UMIs)
Human TCR (with UMIs) product page SMART-Seq Human TCR (with UMIs)
Mouse TCR data SMART-Seq Mouse TCR (with UMIs)
Home › Products › Next-generation sequencing › Bioinformatics tools › Cogent NGS Immune Profiler

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Immune Profiler user manual Cogent NGS Immune Profiler User Manual
Quick Start document CogentIP and Immune Viewer Quick Start Guide
Immune Profiler troubleshooting FAQ Immune Profiler troubleshooting guide
hBCRv2 product page SMART-Seq Human BCR (with UMIs)
Human TCR (with UMIs) product page SMART-Seq Human TCR (with UMIs)
Mouse TCR data SMART-Seq Mouse TCR (with UMIs)

Cogent NGS Immune Profiler Software

Version 1.6.1

Overview

Cogent NGS Immune Profiler is software designed to analyze sequence data stored in FASTQ files generated by Illumina sequencers from libraries prepared using certain Takara Bio immune profiling kits. After analyzing sequence data, the output CSV file can be uploaded to our web-based Cogent NGS Immune Viewer to quickly visualize immune profiling data and generate publication-ready figures.

The Immune Profiler software is free to use, according to the conditions of the End User License Agreement (EULA), by customers who have purchased one of the immune profiling kits:

  • SMART-Seq Human BCR (with UMIs) (Cat. # 634776, 634777 & 634778)
  • SMART-Seq Human TCR (with UMIs) (Cat. # 634779, 634780 & 634781)
  • SMARTer Human BCR IgG IgM H/K/L Profiling Kit (Cat. # 634466 & 634467)
  • SMARTer Human TCR a/b Profiling Kit v2 (Cat. # 634478 & 634479), or
  • SMART-Seq Mouse TCR (with UMIs) (Cat. # 634814, 634815 & 634816)

 

Sign up to get the Cogent NGS Immune Profiler Software

To obtain the software, please review and agree to the End User License Agreement (EULA) and complete the form below. As proof of purchase, please supply your purchase order or invoice number for one of the kits listed above in the specified field.

The information provided will be used to authenticate purchase of one of those kits by your company or organization. After authentication, a link will be provided to download the software.

IMPORTANT: You will need to provide a valid email address or phone number when submitting the form. Without this information, we will be unable to follow up or provide the download link.

After checking the box to indicate acceptance of the EULA and submitting the completed form, the information will be forwarded for purchase verification.


Supported operating systems

  • OS X (El Capitan v10.11 and up)
  • CentOS 6 or higher, RedHat 7.5 or higher

Hardware requirements

  • 16 GB RAM
  • At least 100 GB of available hard drive space (dependent on input FASTQ file size)

Additional third-party software dependencies*

  • Java 1.8–1.15 (Java SE 8–15)
  • Python 3.6 or higher

*Not included with the software; must be downloaded and installed separately.

Additional product information

For use in conjunction with SMART-Seq Human BCR (with UMIs), SMART-Seq Human TCR (with UMIs), SMARTer Human BCR IgG IgM H/K/L Profiling Kit, SMARTer Human TCR a/b Profiling Kit v2, or SMART-Seq Mouse TCR (with UMIs).

More information is available in the Cogent NGS Immune Profiler v1.6 User Manual, or a streamlined protocol can be found in the Cogent NGS Immune Profiler v1.6 and Cogent NGS Immune Viewer v1.0 Quick Start Guide.

Cogent NGS Immune Profiler Software version history
Version number Release date Notes
Cogent NGS Immune Profiler 1.6.1 2023-11-17
  • Updated third-party software implementation
  • New download procedure (documentation-only)
Cogent NGS Immune Profiler 1.6 2023-09-01
  • New kit data input option: SMART-Seq Mouse TCR (with UMIs)
  • Documentation: 
    • User Manual
    • Quick Start Guide
Cogent NGS Immune Profiler 1.5 2022-10-07
  • New kit data input options:
    • SMART-Seq Human BCR (with UMIs)
    • SMART-Seq Human TCR (with UMIs)
  • Additional set of output files compliant with Adaptive Immune Receptor Repertoire (AIRR) Community standards
  • Single installation package—compatible with both Linux and MacOS
  • Documentation: 
    • User Manual
    • Quick Start Guide
Cogent NGS Immune Profiler 1.0 2020-08-04
  • Prerequisites:
    • Requires Java 1.8 or higher
    • Requires Python 3.6 or higher
  • Kit data input options: SMARTer Human BCR IgG IgM H/K/L Profiling Kit, SMARTer Human TCR a/b Profiling Kit v2
  • Documentation: 
    • User Manual
    • Quick Start Guide

Cogent NGS Immune Viewer

Quickly visualize and prepare publication-ready figures of your immune profiling data using output files from the Cogent NGS Immune Profiler.


Featured products

SMART-Seq Human TCR (with UMIs)

SMART-Seq Human TCR (with UMIs) has optimized chemistry, UMIs, and UDIs for more accurate, reliable clonotype calling and quantification.

SMART-Seq Human BCR (with UMIs)

SMART-Seq Human BCR (with UMIs) boasts optimized chemistry, UMIs, and UDIs for more accurate, reliable clonotype calling and quantification.

SMART-Seq Mouse TCR (with UMIs)

SMART-Seq Mouse TCR (with UMIs) enables detection of mouse TCR sequences with sensitivity and no bias.


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