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Immune Profiler user manual Cogent NGS Immune Profiler User Manual
Quick Start document CogentIP and Immune Viewer Quick Start Guide
Immune Profiler troubleshooting FAQ Immune Profiler troubleshooting guide
NGS product page SMART-Seq Human BCR (with UMIs) product page
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Home › Products › Next-generation sequencing › Bioinformatics tools › Cogent NGS Immune Profiler

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Immune Profiler user manual Cogent NGS Immune Profiler User Manual
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Cogent NGS Immune Profiler Software

Version 1.5

Overview

Cogent NGS Immune Profiler is software designed to analyze sequence data stored in FASTQ files generated by Illumina sequencers from libraries prepared using:

  • SMART-Seq Human BCR (with UMIs)
  • SMART-Seq Human TCR (with UMIs)
  • SMARTer Human BCR IgG IgM H/K/L Profiling Kit, or
  • SMARTer Human TCR a/b Profiling Kit v2

After analyzing sequence data with the Immune Profiler, the output CSV file can be uploaded to our web-based Cogent NGS Immune Viewer to quickly visualize immune profiling data and generate publication-ready figures.

If you are interested in using the Cogent NGS Immune Profiler Software, please review the End User License Agreement (EULA), then complete the form below. The Immune Profiler software is free to use according to the conditions of the EULA. Upon acceptance of the EULA and submitting the form, your browser will be directed to a website where you can download the Immune Profiler software. You will access the software as a zip archive containing the following files:

  • Immune Profiler GUI launcher (MacOS only)
  • Command-line interface program (Linux or MacOS)
  • Mini dataset sample files—can be used to test the Immune Profiler functionality (metadata and FASTQ files)
  • Mini dataset sample output reports—compare these files to the results if analyzing the mini dataset sample files

 

Sign up to get the Cogent NGS Immune Profiler Software

If you are interested in using the Cogent NGS Immune Profiler, please review the End User License Agreement (EULA) before populating the required fields of the form.

After checking the box to indicate acceptance of the EULA and submitting the completed form, your browser will be directed to a page where you can download the software.


Supported operating systems

  • OS X (El Capitan v10.11 and up)
  • CentOS 6 or higher, RedHat 7.5 or higher

Hardware requirements

  • 16 GB RAM
  • At least 100 GB of available hard drive space (dependent on input FASTQ file size)

Additional third-party software dependencies*

  • Java 1.8 or higher
  • Python 3.6 or higher

*Not included with the software; must be downloaded and installed separately.

Additional product information

For use in conjunction with SMART-Seq Human BCR (with UMIs), SMART-Seq Human TCR (with UMIs), SMARTer Human BCR IgG IgM H/K/L Profiling Kit, or SMARTer Human TCR a/b Profiling Kit v2. More information is available in the Cogent NGS Immune Profiler v1.5 User Manual, or a streamlined protocol can be found in the Cogent NGS Immune Profiler v1.5 and Cogent NGS Immune Viewer v1.0 Quick Start Guide.

Cogent NGS Immune Profiler Software version history
Version number Release date Notes
Cogent NGS Immune Profiler 1.5 2022-10-07
  • New plate-based kit data input option: SMART-Seq Human BCR (with UMIs)
  • Kit data input option: SMART-Seq Human TCR (with UMIs)
  • Additional set of output files compliant with Adaptive Immune Receptor Repertoire (AIRR) Community standards
  • Single installation package—compatible with both Linux and MacOS
Cogent NGS Immune Profiler 1.0 2020-08-04
  • Prerequisites:
    • Requires Java 1.8 or higher
    • Requires Python 3.6 or higher
  • Kit data input options: SMARTer Human BCR IgG IgM H/K/L Profiling Kit, SMARTer Human TCR a/b Profiling Kit v2
  • Documentation: 
    • User Manual
    • Quick Start Guide

Cogent NGS Immune Viewer

Quickly visualize and prepare publication-ready figures of your immune profiling data using output files from the Cogent NGS Immune Profiler.


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