Delivering true single-cell spatial omics
Meet Trekker—the first true single-cell mapping kit. Designed as a simple reagent kit, Trekker integrates seamlessly with existing single-cell sequencing workflows and preserves the high molecular sensitivity of single-cell data by tagging the nucleus. You can now transform standard single-cell genomics data into spatial data through a simple, straightforward, one-hour workflow incorporated upstream of your library prep.
Meet Trekker—the first true single-cell mapping kit. Designed as a simple reagent kit, Trekker integrates seamlessly with existing single-cell sequencing workflows and preserves the high molecular sensitivity of single-cell data by tagging the nucleus. You can now transform standard single-cell genomics data into spatial data through a simple, straightforward, one-hour workflow incorporated upstream of your library prep.
Trekker works by tagging each nucleus within its native tissue environment with unique spatial barcodes. These spatial barcodes are read using next-generation sequencing (NGS), allowing each nucleus to be bioinformatically positioned in its spatial coordinates. The result is a spatial map with true single-cell resolution without the use of complex instrumentation, cell-type deconvolution, or cell segmentation.
Not sure which Trekker kit to use? Consult our 'Applications and workflows' table or speak with your local sales rep.
Overview
- UMAP to spatial map—fits upstream of your single-cell sequencing workflow
- True single-cell resolution—no deconvolution, segmentation, or complex algorithms
- Simplicity—easy 1-hour workflow without specialized instrumentation
- Same single-cell resolution—preserve the quality and sensitivity of single-cell data
- Beyond spatial transcriptome—explore chromatin accessibility and the immune receptor repertoire
| Applications and workflows |
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| Application | Compatible single-cell or multiomic workflow | Compatible single nucleus capture and library preparation protocol | Compatible Trekker Tile |
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Spatial 3' gene expression |
10x Chromium 3′ RNA, v3.1, v4 |
Trekker Library Preparation with 10x Chromium 3′ Reagent Kit Protocol-At-A-Glance |
Trekker U |
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BD Rhapsody WTA |
Trekker Library Preparation with BD Rhapsody WTA Kit Protocol-At-A-Glance |
Trekker U |
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Illumina Single Cell 3’ RNA Prep |
Trekker U Library Preparation with Illumina Single Cell 3’ RNA Prep Kits Protocol-At-A-Glance |
Trekker U |
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Parse Evercode WT v3 |
Trekker Q Library Preparation with Parse Evercode WT v3 Kits Protocol-At-A-Glance |
Trekker Q |
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Spatial 5' gene expression |
10x Genomics Universal 5' Gene Expression |
Trekker Library Preparation with 10x Chromium 5' Protocol-At-A-Glance |
Trekker 5C |
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Spatial gene expression of FFPE tissues |
10x Single Cell Flex Multiplex with Feature Barcoding |
Trekker FX |
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Spatial gene expression with Trekker 5C and 10x Flex |
10x Single Cell Flex Multiplex with Feature Barcoding |
Trekker 5C Library Preparation with 10x Flex User Guide (User demonstrated) |
Trekker 5C |
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Spatial immune profiling |
BD Rhapsody Single-Cell TCR/BCR Next and mRNA Whole Transcriptome Analysis |
Trekker Library Preparation with BD Rhapsody scTCR/BCR Next and mRNA Whole Transcriptome Analysis User Guide (User demonstrated) |
Trekker U |
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Spatial epigenomics |
BD Rhapsody Single-Cell ATAC-Seq and mRNA Whole Transcriptome Analysis |
Trekker U |
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10x Genomics Epi Multiome ATAC + Gene Expression |
Trekker U Library Preparation with 10x Multiome Kit Protocol-At-A-Glance | Trekker U | |
| Trekker kits and user manuals |
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| Trekker Kit | Compatible training kit | Compatible nuclei tagging and dissociation protocol |
| Trekker 10x10 Training Kit Bundle v2 |
Trekker U Single-Cell Spatial Mapping Kit User Manual | |
| Trekker Q Single-Cell Spatial Mapping Kit User Manual | ||
| Trekker 5C Single-Cell Spatial Mapping Kit User Manual | ||
| Trekker FX 10x10 Training Bundle | Trekker FX Single-Cell Spatial Mapping Kit User Manual | |
| Tissue preparation and dissociation |
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| Preserving and sectioning | Tissue type | Compatible protocol |
| Fresh frozen | For harvesting and preserving fresh tissue and cryostat sectioning | Trekker and Seeker Preserving and Sectioning Tissue Protocol-At-A-Glance |
| FFPE | For processing and sectioning formalin-fixed paraffin embedded (FFPE) human and mouse tissue samples | See Section VI. of the Trekker FX Single-Cell Spatial Mapping Kit User Manual |
| Preparation | Tissue type | Compatible protocol |
| Standard protocol | For most nonfibrous mammalian tissues; recommended starting point for most optimizations |
See Sections V.A–V.D of the appropriate Trekker user manual: Trekker U Single-Cell Spatial Mapping Kit User Manual |
| Neuronal protocol | For myelin protein removal from neuronal tissues where myelin fibers are seen in debris outside nucleus | Trekker Neuronal Tissue Dissociation Protocol-At-A-Glance |
| Alternative protocol for difficult-to-dissociate tissues |
For tumor tissues, many fibrous tissues, including cardiac and skeletal muscle, or any tissues that are difficult to dissociate with the standard protocol |
Trekker Tissue Dissociation for Difficult-to-Dissociate Tissue Types Protocol-At-A-Glance |
More Information
Applications
- Biomarker discovery
- Cancer biology
- Developmental biology
- Immunology
- Neuroscience
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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Seeker Spatial Transcriptomics Kit
Enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution.
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