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Next-generation sequencing

Delivering true single-cell spatial omics

Meet Trekker—the first true single-cell mapping kit. Designed as a simple reagent kit, Trekker integrates seamlessly with existing single-cell sequencing workflows and preserves the high molecular sensitivity of single-cell data by tagging the nucleus. You can now transform standard single-cell genomics data into spatial data through a simple, straightforward, one-hour workflow incorporated upstream of your library prep.

Trekker works by tagging each nucleus within its native tissue environment with unique spatial barcodes. These spatial barcodes are read using next-generation sequencing (NGS), allowing each nucleus to be bioinformatically positioned in its spatial coordinates. The result is a spatial map with true single-cell resolution without the use of complex instrumentation, cell-type deconvolution, or cell segmentation.

Not sure which Trekker kit to use? Consult our 'Applications and workflows' table or speak with your local sales rep.

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Cat. #

Product

Size

Price

License

Quantity

Details

SK017

Trekker™ U 10x10 Bundle

Each

USD $10000.00

The Trekker U 10x10 Bundle contains all the components and reagents necessary to spatially tag four frozen tissue samples.

The bundle includes Trekker U 10x10 Tiles, Nuclei Isolation Buffer, Nuclei Wash Buffer, BSA, RNase Inhibitor, Trekker 10x10 Tile Masking Stickers, 10x10 Wash Chambers, and Trekker 10x10 O-Rings.

Notice to purchaser

Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.

SK020

Trekker™ 10x10 Training Kit Bundle

Each

USD $890.00

The Trekker 10x10 Training Kit Bundle is compatible with Trekker U 10x10 Bundle (Cat. No. SK017) and contains the necessary components to practice, evaluate, and optimize the nuclei dissociation protocol after tissue section placement on tiles. This training bundle is intended to simulate and optimize the tissue sectioning and nuclei dissociation steps, allowing users of the Trekker U tiles to practice and troubleshoot critical processes for two fresh frozen tissue samples.

The bundle includes 10x10 Training Tiles, Nuclei Isolation Buffer, Nuclei Wash Buffer, BSA, Trekker 10x10 Tile Masking Stickers, 10x10 Wash Chambers, and Trekker 10x10 O-Rings.

Notice to purchaser

SK014

Trekker™ 5C 10x10 Bundle

Each

USD $10000.00

License Statement

ID Number

464

PLEASE READ THIS LICENSE CAREFULLY BEFORE USING THE TREKKER™ 5C. To the extent use of the Trekker 5C is covered by any intellectual property rights controlled by Takara Bio USA, Inc. or its affiliates (“Takara”), Takara hereby grants to the customer of the Trekker 5C (“Customer”) a personal, non-exclusive, non-sublicenseable, non-assignable and non-transferable license to such intellectual property rights for the one-time use of the specific Trekker 5C for which Customer has paid the purchase price (“License”). Customer shall have no right and Customer agrees not to transfer, assign or sublicense the License to any other person or entity, and any such attempted transfer, assignment or sublicense shall be void. The License is granted to Customer only, who is the original licensee, and no other person or entity, and is limited to use of the Trekker 5C on a one-time basis and no other product or service.

The Trekker 5C 10x10 Bundle contains all the components and reagents necessary to spatially tag four frozen tissue samples.

The bundle includes Trekker 5C 10x10 Tiles, Nuclei Isolation Buffer, BSA, RNase Inhibitor, Trekker 10x10 Tile Masking Stickers, 10x10 Wash Chambers, and Trekker 10x10 O-Rings.

Notice to purchaser

Components

SK027

Trekker™ Q 10x10 Bundle

4 Rxns

USD $10000.00

The Trekker Q 10x10 Bundle contains all the components and reagents necessary to spatially tag four frozen tissue samples.

The bundle includes Trekker Q 10x10 Tiles, Nuclei Isolation Buffer, BSA, RNase Inhibitor, Trekker 10x10 Tile Masking Stickers, 10x10 Wash Chambers, and Trekker 10x10 O-Rings.

Notice to purchaser

Components

SK031

Trekker™ 10x10 Training Kit Bundle V2

2 Rxns

USD $890.00

The Trekker 10x10 Training Kit Bundle V2 is compatible with Trekker Q 10x10 Bundle (Cat. No. SK027) and Trekker 5C 10x10 Bundle (Cat. No. SK014). It contains the necessary components to practice, evaluate, and optimize the nuclei dissociation protocol after tissue section placement on tiles. This training bundle is intended to simulate and optimize the tissue sectioning and nuclei dissociation steps, allowing users of the Trekker Q and 5C tiles to practice and troubleshoot critical processes for two fresh frozen tissue samples.

The bundle includes 10x10 Training Tiles, Nuclei Isolation Buffer, BSA, Trekker 10x10 Tile Masking Stickers, 10x10 Wash Chambers, and Trekker 10x10 O-Rings.

Notice to purchaser

Components

K011

Trekker™ Starter Kit Bundle (UV Lamp)

Each

USD $1500.00

The Trekker Starter Kit (UV Lamp and Accessories) includes the UV lamp and lamp accessories needed for cleavage of oligonucleotides carrying spatial barcodes from the beads within the Trekker tile.

This catalog number is for the lamp with a Type A (two-prong) adapter common to North America.

Notice to purchaser

K011EUR

Trekker™ Starter Kit Bundle (UV Lamp)

Each

USD $1500.00

The Trekker Starter Kit (UV Lamp and Accessories) includes the UV lamp and lamp accessories needed for cleavage of oligonucleotides carrying spatial barcodes from the beads within the Trekker tile.

This catalog number is for the lamp with an adapter common to Europe (Type C/E/F).

Notice to purchaser

K011UK

Trekker™ Starter Kit Bundle (UV Lamp)

Each

USD $1500.00

The Trekker Starter Kit (UV Lamp and Accessories) includes the UV lamp and lamp accessories needed for cleavage of oligonucleotides carrying spatial barcodes from the beads within the Trekker tile.

This catalog number is for the lamp with a Type G adapter common to the UK.

Notice to purchaser

K011AUS

Trekker™ Starter Kit Bundle (UV Lamp)

Each

USD $1500.00

The Trekker Starter Kit (UV Lamp and Accessories) includes the UV lamp and lamp accessories needed for cleavage of oligonucleotides carrying spatial barcodes from the beads within the Trekker tile.

This catalog number is for the lamp with a Type I adapter common in Australia.

Notice to purchaser

Overview

UMAP to spatial map—fits upstream of your single-cell sequencing workflow
True single-cell resolution—no deconvolution, segmentation, or complex algorithms
Simplicity—easy 1-hour workflow without specialized instrumentation
Same single-cell resolution—preserve the quality and sensitivity of single-cell data
Beyond spatial transcriptome—explore chromatin accessibility and the immune receptor repertoire

Product features
Spatial resolution	True single-cell
Sample type	Fresh frozen tissues
Tile size	10 mm x 10 mm
Specialized capital equipment	None required
Required auxiliary equipment	Cryostat, single-cell sequencing platform, NGS sequencer
Workflow duration	1 hour upstream of single-cell workflow
Sensitivity	Dependent on the single-cell workflow of choice

Applications and workflows
Application	Compatible single-cell or multiomic workflow	Compatible single nucleus capture and library preparation protocol	Compatible Trekker Tile
Spatial 3' gene expression	10x Chromium 3′ RNA, v3.1, v4	Trekker Library Preparation with 10x Chromium 3′ Reagent Kit Protocol-At-A-Glance	Trekker U
	BD Rhapsody WTA	Trekker Library Preparation with BD Rhapsody WTA Kit Protocol-At-A-Glance	Trekker U
	Illumina Single Cell 3’ RNA Prep	Trekker U Library Preparation with Illumina Single Cell 3’ RNA Prep Kits Protocol-At-A-Glance	Trekker U
	Parse Evercode WT v3	Trekker Q Library Preparation with Parse Evercode WT v3 Kits Protocol-At-A-Glance	Trekker Q
Spatial 5' gene expression	10x Genomics Universal 5' Gene Expression	Trekker Library Preparation with 10x Chromium 5' Protocol-At-A-Glance	Trekker 5C
Spatial immune profiling	BD Rhapsody Single-Cell TCR/BCR Next and mRNA Whole Transcriptome Analysis	Trekker Library Preparation with BD Rhapsody scTCR/BCR Next and mRNA Whole Transcriptome Analysis User Guide (User demonstrated)	Trekker U
Spatial epigenomics	BD Rhapsody Single-Cell ATAC-Seq and mRNA Whole Transcriptome Analysis	Trekker U Library Preparation with BD Rhapsody Single-Cell ATAC-Seq and mRNA WTA Kit Protocol-At-A-Glance	Trekker U
Spatial epigenomics	10x Genomics Epi Multiome ATAC + Gene Expression	Trekker U Library Preparation with 10x Multiome Kit Protocol-At-A-Glance	Trekker U

Trekker kits and user manuals
Trekker Kit	Compatible training kit	Compatible nuclei tagging and dissociation protocol
Trekker U 10x10 Bundle	Trekker 10x10 Training Kit Bundle	Trekker U Single-Cell Spatial Mapping Kit User Manual
Trekker Q 10x10 Bundle	Trekker 10x10 Training Kit Bundle v2	Trekker Q Single-Cell Spatial Mapping Kit User Manual
Trekker 5C 10x10 Bundle	Trekker 10x10 Training Kit Bundle v2	Trekker 5C Single-Cell Spatial Mapping Kit User Manual

Tissue preparation protocols
Preparation	Tissue type	Compatible tissue preparation and dissociation protocol
Standard protocol	For most non-fibrous mammalian tissues; recommended starting point for most optimizations	Trekker and Seeker Preserving and Sectioning Tissue Protocol-At-A-Glance
Neuronal protocol	For myelin protein removal from neuronal tissues where myelin fibers are seen in debris outside nucleus	Trekker Neuronal Tissue Dissociation Protocol-At-A-Glance
Cardiac, tumor, and other fibrous tissue protocol	For many fibrous tissues, including cardiac and skeletal muscle, as well as many tumor types	Trekker Cardiac Tissue Dissociation Protocol-At-A-Glance
Alternative protocol for difficult-to-dissociate tissues	For tumor tissues, many fibrous tissues, including cardiac and skeletal muscle, or any tissues that are difficult to dissociate with the standard protocol	Trekker Tissue Dissociation for Difficult-to-Dissociate Tissue Types Protocol-At-A-Glance (coming soon!)

More Information

Applications

Biomarker discovery
Cancer biology
Developmental biology
Immunology
Neuroscience

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Seeker Spatial Transcriptomics Kit

Enable whole transcriptome, spatial mapping of fresh frozen tissues at industry-leading resolution.

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