Embgenix GT-omics Kit
Maximize biological discovery by gaining complete genome and transcriptome information from the same sample—down to single-cell resolution! The comprehensive Embgenix GT-omics Kit combines reproducible PicoPLEX whole-genome amplification (WGA), ultra-sensitive SMART cDNA synthesis, streamlined NGS library preparation, and user-friendly bioinformatics software for end-to-end multiomic analysis.
Ready to embark on your single-cell multiomics journey? Request a quote »
Maximize biological discovery by gaining a complete picture of genomic and transcriptional profiles from the same sample—down to single-cell resolution! The comprehensive Embgenix GT-omics Kit combines reproducible PicoPLEX whole-genome amplification (WGA), ultra-sensitive SMART cDNA synthesis, streamlined NGS library preparation, and user-friendly bioinformatics software for end-to-end multiomic analysis.
The Embgenix GT-omics Kit has been validated for trophectoderm biopsy samples of 3–5 cells and single-cell inputs. The workflow employs bead-based technology to physically separate RNA from DNA after cell lysis. First-strand cDNA synthesis is performed on-bead using SMART (Switching Mechanism at 5′ End of RNA Template) technology optimized for single-cell inputs to provide the highest sensitivity for transcript detection. PicoPLEX WGA technology ensures uniform and reproducible genome amplification from single-cell inputs.
Following cDNA synthesis and whole-genome amplification (WGA), a single-tube workflow without intermediate transfers or clean-up steps is used to prepare RNA-seq and DNA-seq libraries in parallel. The resulting NGS libraries are broadly compatible with Illumina sequencing platforms and allow for multiplexing of up to 96 samples (with one DNA-seq and one RNA-seq library per sample) per sequencing run. After sequencing, data can be analyzed with our easy-to-use Embgenix Analysis Software for CNV analysis and Cogent NGS tools (Analysis and Discovery) for differential expression analysis.
Ready to embark on your single-cell multiomics journey? Request a quote »
Overview
- Reveal how genomic variation correlates with differential gene expression at single-cell resolution to gain new biological insights
- Eliminate the need for splitting precious source material or interpreting across datasets with a combined workflow for both genomic and transcriptomic analysis
- Drive biomarker discovery with industry-leading PicoPLEX WGA and SMART cDNA synthesis technologies optimized for ultra-low or single-cell inputs
- Prevent errors with a single-tube workflow for library prep following cDNA synthesis and whole-genome amplification
- Process sequencing data quickly and easily with free-to-use Embgenix Analysis Software for CNV analysis and Cogent™ NGS Analysis Pipeline (CogentAP) and Cogent NGS Discovery Software (CogentDS) for differential expression analysis
More Information
Applications
- Reproductive health technology
- Oncology biomarker discovery
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Product Components List to determine kit components. Certificates of Analysis and Product Components Lists are located under the Documents tab
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