Home › Products › Next-generation sequencing › RNA-seq › Stranded RNA-seq for mammalian samples › High-input strand-specific total RNA-seq for mammalian samples

RNA-seq

Need help?

Contact Sales

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNA

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours. Because rRNA can comprise ~90% or more of total RNA, effective depletion of rRNA prior to library generation can lower sequencing costs and improve mapping statistics. SMART cDNA synthesis technology followed by PCR complete a streamlined process that performs strand information and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and amplification steps.

These high-input kits contain 12 reverse primers and up to 8 forward primers that allow for multiplexing of up to 96 separate libraries in a high-throughput format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.

Alternate kits for other input amounts:

For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
For 10–100 ng of total RNA, we recommend the SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian

More Less

Cat. #	Product	Size		Quantity
634873	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	12 Rxns	USD $1200.00
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back 634873: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
634874	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	24 Rxns	USD $2039.00
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back 634874: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
634875	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	48 Rxns	USD $3465.00
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back 634875: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
634876	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	96 Rxns	USD $4977.00
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back 634876: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
634877	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	192 Rxns	Inquire for Quotation	*
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back 634877: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
634878	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	480 Rxns	Inquire for Quotation	*
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed. Notice to purchaser Our products are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Documents Components You May Also Like Image Data Resources Back Reproducibility across replicates Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries. Back Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification Back MAQC Analysis MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes. Back Dynamic range and linearity of RNA-seq data Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts. Back Reproducibility across RNA quality Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7. Back Sequence Alignment Metrics Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis. Back High-quality libraries across varying levels of RNA quality High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Overview

Strand information—Identify each transcript’s strand of origin with >99% accuracy.
Fast, streamlined protocol—Go from start to finish in ~5 hours.
Broad input range—Use 100 ng–1 µg of total RNA from human, mouse, or rat.
Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
Seamless integration with Illumina sequencing—Generate up to 192 uniquely indexed Illumina-ready libraries.

Interested in more data and FAQs about this product? Visit the NGS Learning Center.

More Information

Applications

RNA-seq for mammalian samples on Illumina platforms
NGS library generation that retains strand information
Analysis of coding and noncoding sequence information

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. © 2023 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNA

Strand-specific RNA-seq libraries for Illumina platforms from high-input total RNA

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

634873: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

634874: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

634875: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

634876: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

634877: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

Notice to purchaser

Reproducibility across replicates

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

MAQC Analysis

Dynamic range and linearity of RNA-seq data

Reproducibility across RNA quality

Sequence Alignment Metrics

High-quality libraries across varying levels of RNA quality

Overview

More Information

Applications

Additional product information