SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian
NOTE: For strand-specific RNA-seq libraries from high-input total RNA, we recommend the reconfigured versions of the kit below, SMART-Seq Total RNA High Input (RiboGone Mammalian) (Cat. # 635045, 635046 & 635047).
SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634873–634878) will be phased out soon. Contact your local sales representative for more information.
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours.
NOTE: For strand-specific RNA-seq libraries from high-input total RNA, we recommend the reconfigured versions of the kit below, SMART-Seq Total RNA High Input (RiboGone Mammalian) (Cat. # 635045, 635046 & 635047).
SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634873–634878) will be phased out soon. Contact your local sales representative for more information.
The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian provides a rapid solution for preparing indexed Illumina sequencing libraries from 100 ng–1 µg of mammalian total RNA. This kit combines RiboGone and SMART technologies, seamlessly incorporating efficient rRNA removal followed by strand-specific library generation in around 5 hours. Because rRNA can comprise ~90% or more of total RNA, effective depletion of rRNA prior to library generation can lower sequencing costs and improve mapping statistics. SMART cDNA synthesis technology followed by PCR complete a streamlined process that performs strand information and eliminates downstream library prep by incorporating indexes and adapters during the reverse transcription and amplification steps.
These high-input kits contain 12 reverse primers and up to 8 forward primers that allow for multiplexing of up to 96 separate libraries in a high-throughput format. The specific primers you receive will depend on your particular kit. The entire protocol—from rRNA depletion through creation of Illumina-specific, indexed RNA-seq libraries—takes only about 5 hours.
Alternate kits for other input amounts:
- For 250 pg–10 ng of total RNA, we recommend the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian
- For 10–100 ng of total RNA, we recommend the SMART-Seq Total RNA Mid Input
Overview
- Strand information—Identify each transcript’s strand of origin with >99% accuracy.
- Fast, streamlined protocol—Go from start to finish in ~5 hours.
- Broad input range—Use 100 ng–1 µg of total RNA from human, mouse, or rat.
- Versatility—Get reliable data across replicates. See high-quality, reproducible data from total RNA with a RIN (RNA Integrity Number) from 3–10.
- Seamless integration with Illumina sequencing—Generate up to 192 uniquely indexed Illumina-ready libraries.
Interested in more data and FAQs about this product? Visit the NGS Learning Center.
More Information
Applications
- RNA-seq for mammalian samples on Illumina platforms
- NGS library generation that retains strand information
- Analysis of coding and noncoding sequence information
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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