SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

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Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

634873: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Back

Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

634874: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Back

Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

634875: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Back

Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

634876: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Back

Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

634877: SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian

The SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian generates libraries for RNA sequencing (RNA-seq), compatible with Illumina platforms for mammalian samples. The kit is designed to work with input ranges from 100 ng to 1 μg of total RNA. This kit incorporates both RiboGone and SMART technology, and processes ribosomal RNA (rRNA) removal (5S, 5.8S, 18S, and 28S nuclear rRNA sequences), as well as some mitochondrial rRNA sequences (12S), from human, mouse, or rat full-length or sheared total RNA, followed by cDNA synthesis and Illumina library preparation. RNA-seq libraries prepared with this kit are indexed.

Back

Reproducibility across replicates

Reproducibility across replicates. RNA-seq libraries were generated from two samples of 100 ng of HURR. The scatterplot illustrates correlations between the FPKMs (Fragments Per Kilobase Of Exon Per Million Fragments Mapped) from the two libraries.

Back

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation

Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification

Back

MAQC Analysis

MAQC Analysis. RNA-seq libraries were generated with 400 ng of HURR and HUBR. The scatter plot shows the Log₂ ratio of FPKMs of HURR/HBRR graphed against the Log₂ of the ratio of HURR/HBRR derived from qPCR Taqman probes.

Back

Dynamic range and linearity of RNA-seq data

Dynamic range and linearity of RNA-seq data. Libraries were generated from Human Brain Reference RNA with ERCC Spike-In RNA Mix2. The above graph shows strong correlation between the Log₂ of input concentrations of individual ERCC transcripts vs. the Log₂ of FPKMs for those transcripts.

Back

Reproducibility across RNA quality

Reproducibility across RNA quality. A scatterplot illustrates the correlations between the FPKMs from two libraries generated from 1 µg Mouse Liver RNA that was chemically sheared until it had a RIN of 3 or 7.

Back

Sequence Alignment Metrics

Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

Back

High-quality libraries across varying levels of RNA quality

High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.